Fast Preparation of Polydopamine Nanoparticles Catalyzed by Fe²⁺/H₂O₂ for Visible Sensitive Smartphone-Enabled Cytosensing

It is highly desired to develop facile methods for fast preparation of polydopamine nanoparticles (PDANS) for intensive promising applications. Considering the system of Fe 2+ /H 2 O 2 can generate reactive oxygen species efficiently, which can accelerate the self-oxidative polymerization of dopamine, a new time-saving method has been proposed to prepare PDANS catalyzed by Fe 2+ /H 2 O 2 . Thereafter, a novel kind of colorimetric nanoprobe for sensitive detection of human breast cancer cells (MDA-MB-231 cell) based on the obtained PDANS-loaded pH indicator molecules (thymolphthalein) has been developed successfully. The loading capacity of PDANS toward thymolphthalein molecules can reach as high as 165.40 mg/g, which will be a great help to enhancing the sensitivity. Following the color change principle of pH indicators, once simply triggered by basic water, the developed cytosensor offers a visible sensitive smartphone-enabled cytosensing of human breast cancer cells. It has been proved that the rational designed cytosensor is favorable to sensitive detection of cancer cells. By the virtue of its easy use, the proposed smartphone-enabled strategy can provide a novel testing approach for point-of-care bioassay beyond cytosensing in remote areas.