journal article·2022-12-14

Colorimetric Detection of SARS-CoV-2 Using Plasmonic Biosensors and Smartphones

Elsa M. Materón, Faustino R. Gómez, Mariana B. Almeida, Flavio M. Shimizu, Ademar Wong, Kelcilene B. R. Teodoro, Filipe S. R. Silva, Manoel J. A. Lima, Monara Kaelle S. C. Angelim, Matias E. Melendez, Nelson Porras, Pedro M. Vieira, Daniel S. Correa, Emanuel Carrilho, Osvaldo N. Oliveira, Ricardo B. Azevedo, Débora Goncalves

ACS Applied Materials & Interfaces· Volume 14· Issue 49
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Abstract

Low-cost, instrument-free colorimetric tests were developed to detect SARS-CoV-2 using plasmonic biosensors with Au nanoparticles functionalized with polyclonal antibodies (f-AuNPs). Intense color changes were noted with the naked eye owing to plasmon coupling when f-AuNPs form clusters on the virus, with high sensitivity and a detection limit of 0.28 PFU mL–1 (PFU stands for plaque-forming units) in human saliva. Plasmon coupling was corroborated with computer simulations using the finite-difference time-domain (FDTD) method. The strategies based on preparing plasmonic biosensors with f-AuNPs are robust to permit SARS-CoV-2 detection via dynamic light scattering and UV–vis spectroscopy without interference from other viruses, such as influenza and dengue viruses. The diagnosis was made with a smartphone app after processing the images collected from the smartphone camera, measuring the concentration of SARS-CoV-2. Both image processing and machine learning algorithms were found to provide COVID-19 diagnosis with 100% accuracy for saliva samples. In subsidiary experiments, we observed that the biosensor could be used to detect the virus in river waters without pretreatment. With fast responses and requiring small sample amounts (only 20 μL), these colorimetric tests can be deployed in any location within the point-of-care diagnosis paradigm for epidemiological control.
Source: openalex_inverted_index · Quality: baseline

Research context

OpenAlex topics: SARS-CoV-2 detection and testing · Biosensors and Analytical Detection · Advanced biosensing and bioanalysis techniques

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

São Carlos Institute of Physics, University of São Paulo, P.O Box 369, 13560-970São Carlos, SP, Brazil

São Carlos Institute of Chemistry, University of São Paulo, 13566-590São Carlos, SP, Brazil

National Institute of Science and Technology in Bioanalytics - INCTBio, 13083-970Campinas, SP, Brazil

Department of Applied Physics, “Gleb Wataghin” Institute of Physics (IFGW), University of Campinas (UNICAMP), 13083-859Campinas, SP, Brazil

Department of Chemistry, Federal University of São Carlos (UFSCar), 13560-970São Carlos, São Paulo, Brazil

Nanotechnology National Laboratory for Agriculture, Embrapa Instrumentation, 13560-970São Carlos, SP, Brazil

Department of Genetics Evolution, Microbiology, and Immunology, Institute of Biology, University of Campinas, 13083-970Campinas, SP, Brazil

Molecular Carcinogenesis Program, National Cancer Institute, 20231-050Rio de Janeiro, RJ, Brazil

Physics Department, del Valle University, AA 25360Cali, Colombia

Laboratory of Nanobiotechnology, Department of Genetics and Morphology, Institute of Biological Sciences, University of Brasilia, 70910-900Brasilia, DF, Brazil

Funding

Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico · Funda??o de Amparo ? Pesquisa do Estado de S?o Paulo · Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior

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