DataVXACS Applied Materials & InterfacesPaper
journal article·2021-02-24

Sensitive, Rapid, and Automated Detection of DNA Methylation Based on Digital Microfluidics

Qingyu Ruan, Fenxiang Zou, Yang Wang, Yingkun Zhang, Xing Xu, Xiaoye Lin, Tian Tian, Huimin Zhang, Leiji Zhou, Zhi Zhu, Chaoyong Yang

ACS Applied Materials & Interfaces· Volume 13· Issue 7
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Source abstract

Abstract

Biomarkers based on DNA methylation have attracted wide attention in biomedical research due to their potential clinical value. Therefore, a sensitive and accurate method for DNA methylation detection is highly desirable for the discovery and diagnostics of human diseases, especially cancers. Here, an integrated, low-cost, and portable point-of-care (POC) device is presented to analyze DNA methylation, which integrates the process of pyrosequencing in a digital microfluidic chip. Without additional equipment and complicated operation, droplets are manipulated by patterned electrodes with individually programmed control. The system exhibited an excellent sensitivity with a limit of detection (LOD) of 10 pg and a comparable checkout down to 5% methylation level within 30 min, which offered a potential substitute for the detection of DNA methylation. With the advantages of portability, ease of use, high accuracy, and low cost, the POC platform shows great potential for the analysis of tumor-specific circulating DNA.
Source: openalex_inverted_index · Quality: baseline

Research context

OpenAlex topics: Electrowetting and Microfluidic Technologies · Modular Robots and Swarm Intelligence · Advanced Data Storage Technologies

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

The MOE Key Laboratory of Spectrochemical Analysis & Instrumentation, The Key Laboratory of Chemical Biology of Fujian Province, State Key Laboratory of Physical Chemistry of Solid Surfaces, Collaborative Innovation Center of Chemistry for Energy Materials, Department of Chemical Biology, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China

Innovation Laboratory for Sciences and Technologies of Energy Materials of Fujian Province, Xiamen 361005, China

Institute of Molecular Medicine, Renji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200127, China

Funding

Ministry of Education of the People's Republic of China · Ministry of Science and Technology of the People's Republic of China · National Natural Science Foundation of China · Innovative research team of high-level local universities in Shanghai

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