journal article·2020-11-25Open access

Spatial and Temporal Control of CRISPR-Cas9-Mediated Gene Editing Delivered via a Light-Triggered Liposome System

Yagiz Alp Aksoy, Biyao Yang, Wenjie Chen, Tzongtyng Hung, Rhiannon P. Kuchel, Nathan W. Zammit, Shane T. Grey, Ewa M. Goldys, Wei Deng

ACS Applied Materials & Interfaces· Volume 12· Issue 47

Abstract

The CRISPR-Cas9 and related systems offer a unique genome-editing tool allowing facile and efficient introduction of heritable and locus-specific sequence modifications in the genome. Despite its molecular precision, temporal and spatial control of gene editing with the CRISPR-Cas9 system is very limited. We developed a light-sensitive liposome delivery system that offers a high degree of spatial and temporal control of gene editing with the CRISPR-Cas9 system. We demonstrated its efficient protein release by respectively assessing the targeted knockout of the eGFP gene in human HEK293/GFP cells and the TNFAIP3 gene in TNFα-induced HEK293 cells. We further validated our results at a single-cell resolution using an in vivo eGFP reporter system in zebrafish (77% knockout). These findings indicate that light-triggered liposomes may have new options for precise control of CRISPR-Cas9 release and editing.
Source: openalex_inverted_index · Quality: baseline

Research context

OpenAlex topics: CRISPR and Genetic Engineering · RNA Interference and Gene Delivery · Advanced biosensing and bioanalysis techniques

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Diabetes and Metabolism Division, Garvan Institute of Medical Research, Sydney, NSW 2010, Australia

Department of Biomedical Sciences, Faculty of Medicine and Health Sciences, Centre for Motor Neuron Disease Research, Macquarie University, Sydney, NSW 2109, Australia

Sydney Medical School, The University of Sydney, Sydney, NSW 2006, Australia

ARC Centre of Excellence for Nanoscale Biophotonics, Graduate School of Biomedical Engineering, University of New South Wales, Sydney, NSW 2052, Australia

Center for Pharmaceutical Engineering and Sciences, Department of Pharmaceutics, School of Pharmacy, Virginia Commonwealth University, Richmond, Virginia 23298, United States

The Biological Resource Imaging Laboratory, University of New South Wales, Sydney, NSW 2052, Australia

Electron Microscope Unit, University of New South Wales, Sydney, NSW 2052, Australia

Garvan Institute of Medical Research, Sydney, NSW 2010, Australia

Faculty of Medicine, University of New South Wales, Sydney, NSW 2052, Australia

Funding

Australian Research Council

References (70)

Show source-provided references
1. 10.1126/science.122582910.1126/science.1225829 ↗
2. 10.1126/science.125809610.1126/science.1258096 ↗
3. 10.1038/cr.2013.4610.1038/cr.2013.46 ↗
4. 10.1126/science.aat501110.1126/science.aat5011 ↗
5. 10.1038/nm.431310.1038/nm.4313 ↗
6. 10.1038/nrd.2016.28010.1038/nrd.2016.280 ↗
7. 10.1016/j.cell.2015.12.03510.1016/j.cell.2015.12.035 ↗
8. 10.1186/s13073-017-0450-010.1186/s13073-017-0450-0 ↗
9. 10.1038/mt.2016.1010.1038/mt.2016.10 ↗
10. 10.1136/jmedgenet-2018-10542210.1136/jmedgenet-2018-105422 ↗
11. 10.1016/j.stem.2016.04.01310.1016/j.stem.2016.04.013 ↗
12. 10.1073/pnas.180244811510.1073/pnas.1802448115 ↗
13. 10.1021/acs.chemrev.6b0079910.1021/acs.chemrev.6b00799 ↗
14. 10.1002/bies.20130013510.1002/bies.201300135 ↗
15. 10.3389/fpls.2016.0050610.3389/fpls.2016.00506 ↗
16. 10.1038/nbt.173310.1038/nbt.1733 ↗
17. 10.1073/pnas.152024411310.1073/pnas.1520244113 ↗
18. 10.1002/anie.20161020910.1002/anie.201610209 ↗
19. 10.1073/pnas.191222011710.1073/pnas.1912220117 ↗
20. 10.1126/sciadv.aav719910.1126/sciadv.aav7199 ↗
21. 10.1038/cr.2017.1610.1038/cr.2017.16 ↗
22. 10.1016/j.celrep.2018.02.01410.1016/j.celrep.2018.02.014 ↗
23. 10.1038/nbt.347110.1038/nbt.3471 ↗
24. 10.1038/s41551-017-0137-210.1038/s41551-017-0137-2 ↗
25. 10.1038/s41467-018-05118-310.1038/s41467-018-05118-3 ↗
26. 10.18632/oncotarget.1407210.18632/oncotarget.14072 ↗
27. 10.1039/C8TB00994E10.1039/C8TB00994E ↗
28. 10.1021/acs.langmuir.5b0099310.1021/acs.langmuir.5b00993 ↗
29. 10.1016/j.omtn.2017.04.01510.1016/j.omtn.2017.04.015 ↗
30. 10.1016/j.biomaterials.2015.02.00410.1016/j.biomaterials.2015.02.004 ↗
31. 10.1016/j.jconrel.2015.08.00510.1016/j.jconrel.2015.08.005 ↗
32. 10.1007/s12274-017-1719-810.1007/s12274-017-1719-8 ↗
33. 10.1002/advs.20170061110.1002/advs.201700611 ↗
34. 10.1002/adfm.20170303610.1002/adfm.201703036 ↗
35. 10.1039/C8BM01310A10.1039/C8BM01310A ↗
36. 10.1016/j.jconrel.2015.08.00510.1016/j.jconrel.2015.08.005 ↗
37. 10.1002/adma.20150428810.1002/adma.201504288 ↗
38. 10.1038/ncomms454610.1038/ncomms4546 ↗
39. 10.1002/anie.20170868910.1002/anie.201708689 ↗
40. 10.1529/biophysj.106.08238810.1529/biophysj.106.082388 ↗
41. 10.1021/acssynbio.9b0034910.1021/acssynbio.9b00349 ↗
42. 10.1038/s41590-019-0492-010.1038/s41590-019-0492-0 ↗
43. 10.1006/meth.2001.126210.1006/meth.2001.1262 ↗
44. 10.1371/journal.pbio.100116810.1371/journal.pbio.1001168 ↗
45. 10.1242/dev.01571910.1242/dev.015719 ↗
46. 10.1038/s42003-019-0444-010.1038/s42003-019-0444-0 ↗
47. 10.1002/jgm.310710.1002/jgm.3107 ↗
48. 10.1038/nmat244210.1038/nmat2442 ↗
49. 10.1007/s11095-017-2097-310.1007/s11095-017-2097-3 ↗
50. 10.1016/j.bbrc.2012.01.05610.1016/j.bbrc.2012.01.056 ↗
51. 10.1517/1472822090304501810.1517/14728220903045018 ↗
52. 10.1038/ncb360910.1038/ncb3609 ↗
53. 10.2337/db06-014210.2337/db06-0142 ↗
54. 10.1093/jxb/erh23710.1093/jxb/erh237 ↗
55. 10.1016/j.tibtech.2017.11.00610.1016/j.tibtech.2017.11.006 ↗
56. 10.1016/j.jconrel.2017.09.01210.1016/j.jconrel.2017.09.012 ↗
57. 10.1038/nbt.308110.1038/nbt.3081 ↗
58. 10.1016/j.sder.2008.08.00310.1016/j.sder.2008.08.003 ↗
59. 10.1016/j.jphotobiol.2005.01.00810.1016/j.jphotobiol.2005.01.008 ↗
60. 10.1021/mp500129t10.1021/mp500129t ↗
61. 10.1021/nn500777n10.1021/nn500777n ↗
62. 10.1101/gr.171322.11310.1101/gr.171322.113 ↗
63. 10.1016/0304-4165(78)90025-910.1016/0304-4165(78)90025-9 ↗
64. 10.1021/bi00467a00610.1021/bi00467a006 ↗
65. 10.1016/j.ymeth.2003.11.02310.1016/j.ymeth.2003.11.023 ↗
66. 10.1007/s10529-016-2064-910.1007/s10529-016-2064-9 ↗
67. 10.3390/ijms14101977410.3390/ijms141019774 ↗
68. Sakuma T. · Sci. Rep. · 2014 · 4 · 540010.1038/srep05400 ↗
69. 10.1016/j.stem.2013.11.00210.1016/j.stem.2013.11.002 ↗
70. 10.1038/ncomms639310.1038/ncomms6393 ↗