DataVXACS Applied Materials & InterfacesPaper
journal article·2022-06-08

Physically Cross-Linked DNA Hydrogel-Based Sustained Cytokine Delivery for In Situ Diabetic Alveolar Bone Rebuilding

Wei Li, Chengshi Wang, Zhenghao Wang, Liping Gou, Ye Zhou, Ge Peng, Min Zhu, Jiayi Zhang, Ruoqing Li, Hengfan Ni, Lei Wu, Wanli Zhang, Jiaye Liu, Yali Tian, Zhong Chen, Yuan-Ping Han, Nanwei Tong, Xianghui Fu, Xiaofeng Zheng, Per-Olof Berggren

ACS Applied Materials & Interfaces· Volume 14· Issue 22
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Source abstract

Abstract

The development of a biodegradable and shape-adaptable bioscaffold that can enhance local cytokine retention and bioactivity is essential for the application of immunotherapy in periodontal diseases. Here, we report a biodegradable, anti-inflammatory, and osteogenic ILGel that uses a physically cross-linked DNA hydrogel as a soft bioscaffold for the long-term sustained release of cytokine interleukin-10 (IL-10) to accelerate diabetic alveolar bone rebuilding. Porous microstructures of ILGel favored the encapsulation of IL-10 and maintained IL-10 bioactivity for at least 7 days. ILGel can be gradually degraded or hydrolyzed under physiological conditions, avoiding the potential undesired side effects on dental tissues. Long-term sustained release of bioactive IL-10 from ILGel not only promoted M2 macrophage polarization and attenuated periodontal inflammation but also triggered osteogenesis of mesenchymal stem cells (MSCs), leading to accelerated alveolar bone formation and healing of alveolar bone defects under diabetic conditions in vivo. ILGel treatment significantly accelerated the defect healing rate of diabetic alveolar injury up to 93.42 ± 4.6% on day 21 post treatment compared to that of free IL-10 treatment (63.30 ± 7.39%), with improved trabecular architectures. Our findings imply the potential application of the DNA hydrogel as the bioscaffold for cytokine-based immunotherapy in diabetic alveolar bone injury and other periodontal diseases.
Source: openalex_inverted_index · Quality: baseline

Research context

PolymersBiomaterials

OpenAlex topics: Oral microbiology and periodontitis research · Immune Response and Inflammation · Mesenchymal stem cell research

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Department of Endocrinology and Metabolism, Center for Diabetes and Metabolism Research, West China Hospital, Sichuan University, Chengdu 610041, China

The Rolf Luft Research Center for Diabetes and Endocrinology, Karolinska Institutet, SE-17176 Stockholm, Sweden

Laboratory of Stem Cell and Tissue Engineering, Orthopedic Research Institute, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu 610041, China

Department of General Medicine, Chongqing University Central Hospital, Chongqing Emergency Medical Center, Chongqing 400014, China

Department of Pharmacy, West China Hospital, Sichuan University, Chengdu 610041, China

Core Facility of West China Hospital, Sichuan University, Chengdu 610041, China

Department of Thyroid and Parathyroid Surgery, West China Hospital, Sichuan University, Chengdu 610041, China

West China School of Nursing, West China Hospital, Sichuan University, Chengdu 610041, China

Department of Spinal Surgery, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China

The Center for Growth, Metabolism and Aging, The College of Life Sciences, Sichuan University, Chengdu 610065, China

Division of Endocrinology and Metabolism, National Clinical Research Center for Geriatrics, State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University and Collaborative Innovation Center of Biotherapy, Chengdu 610041, China

Funding

National Natural Science Foundation of China · Center of Excellence-International Collaboration Initiative Grant of West China Hospital · the Post-Doctor Research Project, West China Hospital of Sichuan University · Program for Overseas High-Level Talents Introduction of Sichuan Province of China · China Postdoctoral Science Foundation · 1.3.5 project for disciplines of excellence, West China Hospital of Sichuan University · Grant from Technology Innovation R & D Project of Chengdu Science and Technology Bureau

References (32)

Show source-provided references
1. 10.1111/j.1600-0501.2010.01992.x10.1111/j.1600-0501.2010.01992.x ↗
2. 10.1034/j.1600-051x.2000.027008567.x10.1034/j.1600-051x.2000.027008567.x ↗
3. 10.1007/s11914-015-0286-810.1007/s11914-015-0286-8 ↗
4. 10.1038/ijos.2015.210.1038/ijos.2015.2 ↗
5. 10.1016/j.bpj.2019.05.01910.1016/j.bpj.2019.05.019 ↗
6. 10.1016/j.immuni.2019.03.02010.1016/j.immuni.2019.03.020 ↗
7. 10.1126/sciadv.aaz074810.1126/sciadv.aaz0748 ↗
8. 10.1146/annurev.immunol.19.1.68310.1146/annurev.immunol.19.1.683 ↗
9. 10.1155/2014/28483610.1155/2014/284836 ↗
10. 10.1111/j.1600-0722.2006.00283.x10.1111/j.1600-0722.2006.00283.x ↗
11. 10.1186/1471-2121-8-410.1186/1471-2121-8-4 ↗
12. 10.1111/j.1600-0722.2009.00706.x10.1111/j.1600-0722.2009.00706.x ↗
13. 10.1186/s13287-020-1578-110.1186/s13287-020-1578-1 ↗
14. 10.1016/j.jpba.2021.11390010.1016/j.jpba.2021.113900 ↗
15. 10.1016/j.actbio.2019.01.00110.1016/j.actbio.2019.01.001 ↗
16. 10.1016/j.biomaterials.2017.01.01610.1016/j.biomaterials.2017.01.016 ↗
17. 10.1002/adhm.20160125110.1002/adhm.201601251 ↗
18. 10.1177/002203451983193110.1177/0022034519831931 ↗
19. 10.1021/acsbiomaterials.0c0014510.1021/acsbiomaterials.0c00145 ↗
20. 10.1016/j.addr.2020.07.01810.1016/j.addr.2020.07.018 ↗
21. 10.1021/acsami.9b0865210.1021/acsami.9b08652 ↗
22. 10.1021/acs.chemrev.0c0029410.1021/acs.chemrev.0c00294 ↗
23. 10.1021/acs.nanolett.7b0100610.1021/acs.nanolett.7b01006 ↗
24. 10.1002/smll.20130005510.1002/smll.201300055 ↗
25. 10.1902/jop.2013.13015510.1902/jop.2013.130155 ↗
26. 10.1111/jcmm.1500510.1111/jcmm.15005 ↗
27. 10.1016/j.redox.2021.10196310.1016/j.redox.2021.101963 ↗
28. 10.1016/j.cyto.2016.06.01810.1016/j.cyto.2016.06.018 ↗
29. Gong L. · Ann. Clin. Lab. Sci. · 2016 · 46 · 65
30. 10.3390/nu1210299910.3390/nu12102999 ↗
31. 10.1016/j.bone.2020.11555810.1016/j.bone.2020.115558 ↗
32. 10.1021/acsnano.1c0727010.1021/acsnano.1c07270 ↗