DataVXACS Applied Materials & InterfacesPaper
journal article·2020-06-17

In Situ Detection of Endotoxin in Bacteriostatic Process by SERS Chip Integrated Array Microchambers within Bioscaffold Nanostructures and SERS Tags

Songtao Xiang, Chuang Ge, Shunbo Li, Li Chen, Li Wang, Yi Xu

ACS Applied Materials & Interfaces
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Source abstract

Abstract

In order to achieve real-time and in situ detection of endotoxin, which is an important and significant clinical test index, surface-enhanced Raman spectroscopy (SERS) chip integrated array microchambers within bioscaffold nanostructures and a SERS monitoring strategy were proposed in this paper. After sputtering of nanogold on the cicada wing, which was selected as a natural template, and polydimethylsiloxane bonding, array-type chambers within bioscaffold nanostructures were prepared for in situ bacterial culture and monitoring of endotoxin in the bacteriostasis process by SERS. Meanwhile, the SERS tag modified with the DNA aptamer was prepared and added into this complex biochemical reaction to further improve the sensitivity and selectivity. A new method for in situ detection of endotoxin was thus established. The detection time was shortened to 100 s, and the detection limit was as low as 6.25 ng/mL. Pseudomonas aeruginosa was cultured in situ in the chamber of the SERS chip with antimicrobial agents in 0–72 h. The endotoxin released in the antibacterial process was monitored by the designed SERS detection strategy. The results obtained by SERS analysis were consistent with those of the ELISA kit.
Source: openalex_inverted_index · Quality: baseline

Research context

Nanomaterials

OpenAlex topics: Biosensors and Analytical Detection · Advanced biosensing and bioanalysis techniques · Gold and Silver Nanoparticles Synthesis and Applications

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Key Disciplines Lab of Novel Micro−Nano Devices and System Technology, Key Laboratory of Optoelectronic Technology and Systems, Ministry of Education, Chongqing University, Shapingba, Chongqing 400044, China

School of Chemistry and Chemical Engineering, Chongqing University, Shapingba, Chongqing 400044, China

International R & D Center of Micro−nano Systems and New Materials Technology, Chongqing University, Shapingba, Chongqing 400044, China

Chongqing Key Laboratory of Translational Research for Cancer Metastasis and Individualized Treatment, Chongqing University Cancer Hospital, Shapingba, Chongqing 400030, China

School of Optoelectronic Engineering, Chongqing University, Shapingba, Chongqing 400044, China

Funding

Ministry of Education of the People's Republic of China · National Natural Science Foundation of China · Major Project of Technological Innovation and Application Demonstration of Chongqing · Chongqing Artificial Intelligence Technology Innovation Major Special Project

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