journal article·2018-10-17

Ultrasensitive Detection of Serum MicroRNA Using Branched DNA-Based SERS Platform Combining Simultaneous Detection of α-Fetoprotein for Early Diagnosis of Liver Cancer

Linxiu Cheng, Zhikun Zhang, Duo Zuo, Wenfeng Zhu, Jie Zhang, Qingdao Zeng, Dayong Yang, Min Li, Yuliang Zhao

ACS Applied Materials & Interfaces· Volume 10· Issue 41
Publisher page

Abstract

We provided an ultrasensitive sensing strategy for microRNA detection by first employing branched DNA. With the aid of microcontact printing, we realized the multiplex sensing of different kinds of liver cancer biomarkers: microRNA and protein simultaneously. Delicately designed branched DNA included multiple complementary sticky ends as probe to microRNA capture and the double-stranded rigid branched core to increase the active sticky-ends distance and expose more DNA probes for sensitivity. The branched DNA enables 2 orders of magnitude increase in sensitivity for microRNA detection over single-stranded DNA. The limit of detection reaches as low as 10 attomolar (S/N = 3) for miR-223 and 10–12 M for α-fetoprotein. In addition, this system shows high selectivity and appropriate reproducibility (the relative standard deviation is less than 20%) in physiological media. Serum samples are tested and the results of α-fetoprotein are in good agreement with the current gold-standard method, electrochemiluminescence immunoassay analyzer. The results suggest the reliability of this approach in physiological media and show high potential in the sensing of low abundant microRNA in serum, especially for early diagnosis of primary liver cancers.
Source: openalex_inverted_index · Quality: baseline

Research context

OpenAlex topics: Extracellular vesicles in disease · MicroRNA in disease regulation · Advanced biosensing and bioanalysis techniques

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

CAS Key Laboratory for Biomedical Effects of Nanomaterials & Nanosafety, Institute of High Energy Physics, Chinese Academy of Sciences, 19B, Yuquan Road, Shijingshan District, Beijing 100049, China

CAS Key Laboratory of Standardization and Measurement for Nanotechnology, CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology (NCNST), Beijing 100190, China

University of Chinese Academy of Sciences, Beijing 100049, China

School of Chemical Engineering and Technology, Key Laboratory of Systems Bioengineering (Ministry of Education), Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Tianjin University, Tianjin 300072, China

Department of Clinical Laboratory, Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer, Key Laboratory of Cancer Prevention and Therapy, Tianjin’s Clinical Research Center for Cancer, Tianjin 300060, China

Funding

Ministry of Science and Technology of the People's Republic of China · National Natural Science Foundation of China · Tianjin Medical University Cancer Institute and Hospital

References (56)

Show source-provided references
1. 10.1038/nrc344910.1038/nrc3449 ↗
2. 10.1002/ijc.3021110.1002/ijc.30211 ↗
3. 10.3322/caac.2133810.3322/caac.21338 ↗
4. 10.1016/j.canlet.2012.07.02410.1016/j.canlet.2012.07.024 ↗
5. 10.1038/178794a010.1038/178794a0 ↗
6. 10.1200/JCO.2009.23.128210.1200/JCO.2009.23.1282 ↗
7. 10.1111/jgh.1246110.1111/jgh.12461 ↗
8. 10.1038/bjc.2014.53610.1038/bjc.2014.536 ↗
9. 10.1002/cncr.2525710.1002/cncr.25257 ↗
10. 10.1074/mcp.M113.03125210.1074/mcp.M113.031252 ↗
11. 10.1007/s13277-015-4545-110.1007/s13277-015-4545-1 ↗
12. 10.1021/cr300362f10.1021/cr300362f ↗
13. 10.1002/anie.20150972610.1002/anie.201509726 ↗
14. 10.1021/acsami.7b0942010.1021/acsami.7b09420 ↗
15. 10.1021/acssensors.7b0092210.1021/acssensors.7b00922 ↗
16. 10.1021/acsami.7b0657910.1021/acsami.7b06579 ↗
17. 10.1021/acs.chemrev.5b0032110.1021/acs.chemrev.5b00321 ↗
18. 10.1038/cr.2014.4410.1038/cr.2014.44 ↗
19. 10.1038/nature1458110.1038/nature14581 ↗
20. 10.1021/ja311313b10.1021/ja311313b ↗
21. 10.1002/anie.20170736610.1002/anie.201707366 ↗
22. 10.1021/acs.analchem.7b0186110.1021/acs.analchem.7b01861 ↗
23. 10.1021/acs.analchem.7b0324010.1021/acs.analchem.7b03240 ↗
24. 10.1021/ja412152x10.1021/ja412152x ↗
25. 10.1002/ijc.3002010.1002/ijc.30020 ↗
26. 10.1016/j.bios.2013.06.05410.1016/j.bios.2013.06.054 ↗
27. 10.1016/j.bios.2013.10.00910.1016/j.bios.2013.10.009 ↗
28. 10.1039/c0an00704h10.1039/c0an00704h ↗
29. 10.1021/acsnano.7b0153610.1021/acsnano.7b01536 ↗
30. 10.1039/C6AN01076H10.1039/C6AN01076H ↗
31. 10.1038/ncomms1343710.1038/ncomms13437 ↗
32. 10.1038/s41570-017-006010.1038/s41570-017-0060 ↗
33. 10.1021/acs.analchem.5b0118610.1021/acs.analchem.5b01186 ↗
34. 10.1039/c1cc15707h10.1039/c1cc15707h ↗
35. 10.1038/srep0407510.1038/srep04075 ↗
36. 10.1021/ja909088510.1021/ja9090885 ↗
37. 10.1021/nl101946c10.1021/nl101946c ↗
38. 10.1039/c3an01600e10.1039/c3an01600e ↗
39. 10.1021/nn300352b10.1021/nn300352b ↗
40. 10.1021/acsnano.5b0600710.1021/acsnano.5b06007 ↗
41. 10.1021/ja405120x10.1021/ja405120x ↗
42. 10.1002/wnan.11110.1002/wnan.111 ↗
43. 10.1021/ar500108210.1021/ar5001082 ↗
44. 10.1002/anie.20130217510.1002/anie.201302175 ↗
45. 10.1038/nmat104510.1038/nmat1045 ↗
46. 10.1039/c2nr12039a10.1039/c2nr12039a ↗
47. 10.1002/anie.20141072010.1002/anie.201410720 ↗
48. 10.1021/ac034356f10.1021/ac034356f ↗
49. 10.1039/C2AN36128K10.1039/C2AN36128K ↗
50. 10.1021/ac050415910.1021/ac0504159 ↗
51. 10.1039/b708461g10.1039/b708461g ↗
52. 10.1063/1.499210610.1063/1.4992106 ↗
53. Hodkiewicz J. · 2010
54. 10.1021/la047586d10.1021/la047586d ↗
55. 10.1016/j.jhazmat.2016.09.02510.1016/j.jhazmat.2016.09.025 ↗
56. 10.1016/j.bios.2015.06.00310.1016/j.bios.2015.06.003 ↗