Novel ZnO/N-halamine-Mediated Multifunctional Dressings as Quick Antibacterial Agent for Biomedical Applications

Cutaneous hemorrhage often occurs in daily life which may cause infection and even amputation. This research aims to develop a novel chitosan dressing impregnated with ZnO/N-halamine hybrid nanoparticles for quick antibacterial performance, outstanding hemostatic potential, high porosity, and favorable swelling property through combining sonication and lyophilization processing. After 30 days of storage, about 90% bacterial cell viability loss could be observed toward both Gram-positive Staphylococcus aureus and Gram-negative Escherichia coli O157:H7 within 30 min of contact by colony counting method. The hybrids assembled much more platelet and red blood cell as compared with pure chitosan control. Moreover, the lower blooding clotting index value gave evidence that these composites could control hemorrhaging and reduce the probability of wound infection. No potential skin irritation and toxicity were detected using in vitro cytocompatibility and a skin stimulation test. Therefore, this work demonstrated a facile and cost-effective approach for the preparation of N-halamine-based hybrid sponges which show promising application for wound dressings.