journal article·2019-08-28

Engineering DNA–Nanozyme Interfaces for Rapid Detection of Dental Bacteria

Ling Zhang, Zhengnan Qi, Yan Zou, Jiaxing Zhang, Wenjun Xia, Rui Zhang, Zhiyan He, Xiaoxiao Cai, Yunfeng Lin, Sheng-Zhong Duan, Jiang Li, Lihua Wang, Na Lu, Zisheng Tang

ACS Applied Materials & Interfaces· Volume 11· Issue 34
Publisher page

Abstract

Engineering biological interfaces represents a powerful means to improve the performance of biosensors. Here, we developed a DNA-engineered nanozyme interface for rapid and sensitive detection of dental bacteria. We employed DNA aptamer as both molecular recognition keys and adhesive substrates to functionalize the nanozyme. Utilizing different immobilization strategies and DNA designs, a range of DNA nanoscale biointerfaces were constructed to modulate enzymatic and biological properties of the nanozyme systems. These functional biointerfaces improved the accessibility of bacteria to the nanozyme surface, providing large signal change range at optimal DNA probe density. The DNA-functionalized nanozymes demonstrate a rapid, label-free, and highly sensitive direct colorimetric detection of Streptococcus mutans, with a detection limit of 12 CFU mL–1, as well as excellent discrimination from other dental bacteria. We demonstrate the use of this biological nanointerface for identifying dental bacteria in salivary samples, showing its potential in clinical prevention and diagnosis of dental diseases.
Source: openalex_inverted_index · Quality: baseline

Research context

OpenAlex topics: Advanced Nanomaterials in Catalysis · Advanced biosensing and bioanalysis techniques · Nanoplatforms for cancer theranostics

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

National Clinical Research Center of Oral Diseases, Shanghai 200011, China

Shanghai Key Laboratory of Stomatology & Shanghai Research Institute of Stomatology, Shanghai 200011, China

Department of Oral Medicine, Shanghai Stomatological Hospital, Fudan University, Shanghai 200031, China

Oral Biomedical Engineering Laboratory, Shanghai Stomatological Hospital, Fudan University, Shanghai 200031, China

School of Materials Engineering, Shanghai University of Engineering Science, Shanghai 201620, China

State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China

Zhangjiang Laboratory, Shanghai Advanced Research Institute, Chinese Academy of Sciences, Shanghai 201210, China

Funding

Natural Science Foundation of Shanghai · Ministry of Science and Technology of the People's Republic of China · Shanghai University of Engineering and Science · National Natural Science Foundation of China · Chinese Academy of Sciences

References (54)

Show source-provided references
1. 10.1038/nmat96410.1038/nmat964 ↗
2. 10.1021/acsnano.7b0672810.1021/acsnano.7b06728 ↗
3. 10.1002/adfm.20170274810.1002/adfm.201702748 ↗
4. 10.1021/acsami.5b0106910.1021/acsami.5b01069 ↗
5. 10.1007/s12274-019-2313-z10.1007/s12274-019-2313-z ↗
6. 10.1021/ar400250z10.1021/ar400250z ↗
7. 10.1039/C7DT01155E10.1039/C7DT01155E ↗
8. 10.1039/c3cs35486e10.1039/c3cs35486e ↗
9. 10.1021/acs.chemrev.8b0067210.1021/acs.chemrev.8b00672 ↗
10. 10.1039/C5QI00240K10.1039/C5QI00240K ↗
11. 10.1039/C8NR09816F10.1039/C8NR09816F ↗
12. 10.1002/adma.20070191810.1002/adma.200701918 ↗
13. 10.1016/j.snb.2018.11.00510.1016/j.snb.2018.11.005 ↗
14. 10.1002/adma.20140045110.1002/adma.201400451 ↗
15. 10.1021/nl801596a10.1021/nl801596a ↗
16. 10.1007/s12274-017-1919-210.1007/s12274-017-1919-2 ↗
17. 10.1039/C6NR05078F10.1039/C6NR05078F ↗
18. 10.1039/C7DT04491G10.1039/C7DT04491G ↗
19. 10.1039/C7CC07149C10.1039/C7CC07149C ↗
20. 10.1021/acsami.7b0730710.1021/acsami.7b07307 ↗
21. 10.1039/C7NR00819H10.1039/C7NR00819H ↗
22. 10.1002/nbm.290910.1002/nbm.2909 ↗
23. 10.1016/j.biomaterials.2014.04.05210.1016/j.biomaterials.2014.04.052 ↗
24. 10.1016/j.biomaterials.2015.02.01910.1016/j.biomaterials.2015.02.019 ↗
25. 10.1039/C5CS00541H10.1039/C5CS00541H ↗
26. 10.1039/C7NR07035G10.1039/C7NR07035G ↗
27. 10.1016/j.biomaterials.2017.05.01310.1016/j.biomaterials.2017.05.013 ↗
28. 10.1002/cjoc.20169000810.1002/cjoc.201690008 ↗
29. 10.1002/anie.20141089510.1002/anie.201410895 ↗
30. 10.1002/adfm.20170274810.1002/adfm.201702748 ↗
31. 10.1021/acs.analchem.8b0395910.1021/acs.analchem.8b03959 ↗
32. 10.1126/science.295.5559.148710.1126/science.295.5559.1487 ↗
33. 10.1038/nrmicro241510.1038/nrmicro2415 ↗
34. 10.1038/srep1440210.1038/srep14402 ↗
35. 10.1021/jacs.6b0075110.1021/jacs.6b00751 ↗
36. 10.1093/nsr/nwx13410.1093/nsr/nwx134 ↗
37. 10.1007/s12274-017-1426-510.1007/s12274-017-1426-5 ↗
38. 10.1146/annurev-anchem-061417-01000710.1146/annurev-anchem-061417-010007 ↗
39. 10.1021/acs.analchem.7b0333510.1021/acs.analchem.7b03335 ↗
40. 10.1021/cs501580510.1021/cs5015805 ↗
41. 10.1039/C3CS60331H10.1039/C3CS60331H ↗
42. 10.1002/anie.20120235610.1002/anie.201202356 ↗
43. 10.1016/S0140-6736(07)60031-210.1016/S0140-6736(07)60031-2 ↗
44. 10.1177/002203451037960210.1177/0022034510379602 ↗
45. 10.1126/science.284.5413.44910.1126/science.284.5413.449 ↗
46. Loesche W. J. · J. Clin. Microbiol. · 1992 · 30 · 41810.1128/jcm.30.2.418-426.1992 ↗
47. 10.1038/nnano.2007.26010.1038/nnano.2007.260 ↗
48. 10.1002/bit.2511110.1002/bit.25111 ↗
49. 10.1039/C5NR04176G10.1039/C5NR04176G ↗
50. 10.1021/acsami.8b1607510.1021/acsami.8b16075 ↗
51. 10.1039/C7NR05541B10.1039/C7NR05541B ↗
52. 10.1039/b810812a10.1039/b810812a ↗
53. 10.1021/ac000662710.1021/ac0006627 ↗
54. 10.1002/anie.20180553210.1002/anie.201805532 ↗