DataVXACS Applied Materials & InterfacesPaper
journal article·2021-05-26

3D-Bioprinted Difunctional Scaffold for In Situ Cartilage Regeneration Based on Aptamer-Directed Cell Recruitment and Growth Factor-Enhanced Cell Chondrogenesis

Zhen Yang, Tianyuan Zhao, Cangjian Gao, Fuyang Cao, Hao Li, Zhiyao Liao, Liwei Fu, Pinxue Li, Wei Chen, Zhiqiang Sun, Shuangpeng Jiang, Zhuang Tian, Guangzhao Tian, Kangkang Zha, Tingting Pan, Xu Li, Xiang Sui, Zhiguo Yuan, Shuyun Liu, Quanyi Guo

ACS Applied Materials & Interfaces· Volume 13· Issue 20
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Source abstract

Abstract

Articular cartilage (AC) lesions are fairly common but remain an obstacle for clinicians and researchers due to their poor self-healing capacity. Recently, a promising therapy based on the recruitment of autologous mesenchymal stem cells (MSCs) has been developed for the regeneration of full-thickness cartilage defects in the knee joint. In this study, a 3D-bioprinted difunctional scaffold was developed based on aptamer HM69-mediated MSC-specific recruitment and growth factor-enhanced cell chondrogenesis. The aptamer, which can specifically recognize and recruit MSCs, was first chemically conjugated to the decellularized cartilage extracellular matrix and then mixed with gelatin methacrylate to form a photocrosslinkable bioink ready for 3D bioprinting. Together with the growth factor that promoted cell chondrogenic differentiation, the biodegradable polymer poly(ε-caprolactone) was further chosen to impart mechanical strength to the 3D bioprinted constructs. The difunctional scaffold specifically recruited MSCs, provided a favorable microenvironment for cell adhesion and proliferation, promoted chondrogenesis, and thus greatly improved cartilage repair in rabbit full-thickness defects. In conclusion, this study demonstrated that 3D bioprinting of difunctional scaffolds could be a promising strategy for in situ AC regeneration based on aptamer-directed cell recruitment and growth-factor-enhanced cell chondrogenesis.
Source: openalex_inverted_index · Quality: baseline

Research context

Polymers

OpenAlex topics: Osteoarthritis Treatment and Mechanisms · Knee injuries and reconstruction techniques · 3D Printing in Biomedical Research

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Beijing Key Lab of Regenerative Medicine in Orthopedics; Key Laboratory of Musculoskeletal Trauma & War Injuries PLA, Institute of Orthopedics, the First Medical Center, Chinese PLA General Hospital, No. 28 Fuxing Road, Haidian District, Beijing 100853, China

School of Medicine, Nankai University, Tianjin 300071, China

Department of Orthopedics, The First Affiliated Hospital of Zhengzhou University, 1 Jian East Road, Eqi District, Zhengzhou 450052, China

The First Hospital of China Medical University, 155 Nanjing North Street, Heping District, Shenyang, 110001 Liaoning Province, China

Birth Defects Prevention and Control Technology Research Center, Center for Health Research and Innovation, Chinese PLA General Hospital, Beijing 100853, China

Musculoskeletal Research Laboratory, Department of Orthopedics & Traumatology, The Chinese University of Hong Kong, Hong Kong, China; Li Ka Shing Health and Science Institute, The Chinese University of Hong Kong, Hong Kong, China; Joint Laboratory of Chinese Academic of Science and Hong Kong for Biomaterials, The Chinese University of Hong Kong, Hong Kong 999077, China

Department of Bone and Joint Surgery, Renji Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 200001, China

Funding

Ministry of Science and Technology of the People's Republic of China · National Natural Science Foundation of China · Chinese PLA General Hospital Medical Youth Program

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