Self-Assembled Double-Bundle DNA Tetrahedron for Efficient Antisense Delivery
Source abstract
Abstract
DNA nanostructures are promising biomaterials capable of arranging multiple functional components with nanometer precision. Here, a double-bundle DNA tetrahedron is rationally designed to integrate with antisense oligonucleotides silencing proto-oncogene c-raf and nuclear targeting peptides. The functionalized DNA tetrahedron can be internalized by A549 cells and assists the delivery of antisense oligonucleotides toward the nucleus to increase the chance to downregulate target mRNA in nucleus and cytoplasm. Antisense strands released from the tetrahedron in response to the intracellular reducing environment can inhibit cell proliferation at a low concentration without transfection reagent. Finally, efficient knockdown of c-raf gene is observed, which verified our design. This designer DNA-based nanocarrier system will open a new avenue for efficient delivery of nucleic acid drugs.
Source: openalex_inverted_index · Quality: baseline
Research context
OpenAlex topics: Advanced biosensing and bioanalysis techniques · RNA Interference and Gene Delivery · DNA and Nucleic Acid Chemistry
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Bio-X Institutes, Key Laboratory for the Genetics of Developmental and Neuropsychiatric Disorders (Ministry of Education), Shanghai Jiao Tong University, Shanghai 200240, China
CAS Key Laboratory of Nanosystem and Hierarchical Fabrication, CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology, Beijing 100190, China
University of Chinese Academy of Sciences, Beijing 100049, China
Funding
???the National Basic Research Programs of China
References (43)
Show source-provided references
1. 10.1038/nature0140610.1038/nature01406 ↗
2. 10.1038/nnano.2011.18710.1038/nnano.2011.187 ↗
3. 10.1038/ncomms133710.1038/ncomms1337 ↗
4. 10.1038/nnano.2012.7310.1038/nnano.2012.73 ↗
5. 10.1016/j.biomaterials.2013.03.07410.1016/j.biomaterials.2013.03.074 ↗
6. 10.1021/nl301877k10.1021/nl301877k ↗
7. 10.1021/nn202774x10.1021/nn202774x ↗
8. 10.1038/nnano.2015.19510.1038/nnano.2015.195 ↗
9. 10.1038/nmat150810.1038/nmat1508 ↗
10. 10.1002/smll.20110180410.1002/smll.201101804 ↗
11. 10.1021/ja304263n10.1021/ja304263n ↗
12. 10.1021/acsami.8b0083310.1021/acsami.8b00833 ↗
13. 10.1039/C7NR09692E10.1039/C7NR09692E ↗
14. 10.1039/C7CC09397G10.1039/C7CC09397G ↗
15. 10.1016/j.addr.2015.02.00210.1016/j.addr.2015.02.002 ↗
16. 10.1038/372333a010.1038/372333a0 ↗
17. 10.1126/science.835151510.1126/science.8351515 ↗
18. 10.1016/S0140-6736(01)05629-X10.1016/S0140-6736(01)05629-X ↗
19. 10.1093/nar/gkh51610.1093/nar/gkh516 ↗
20. 10.1038/nrd83710.1038/nrd837 ↗
21. 10.1016/j.addr.2015.02.00710.1016/j.addr.2015.02.007 ↗
22. 10.1016/S0169-409X(02)00017-010.1016/S0169-409X(02)00017-0 ↗
23. 10.1093/nar/gkn34210.1093/nar/gkn342 ↗
24. 10.1073/pnas.100391910710.1073/pnas.1003919107 ↗
25. 10.1002/anie.20090435910.1002/anie.200904359 ↗
26. 10.1021/ja512293f10.1021/ja512293f ↗
27. 10.1021/acs.chemmater.6b0254610.1021/acs.chemmater.6b02546 ↗
28. 10.1039/C7CS00822H10.1039/C7CS00822H ↗
29. 10.1016/j.jconrel.2016.10.01510.1016/j.jconrel.2016.10.015 ↗
30. 10.1021/acs.biochem.5b0118110.1021/acs.biochem.5b01181 ↗
31. 10.1021/nn200557410.1021/nn2005574 ↗
32. 10.1016/j.jconrel.2018.04.05110.1016/j.jconrel.2018.04.051 ↗
33. 10.1021/jacs.7b1277210.1021/jacs.7b12772 ↗
34. 10.1002/adma.20160197610.1002/adma.201601976 ↗
35. 10.1038/nature0659710.1038/nature06597 ↗
36. 10.1002/anie.20140323610.1002/anie.201403236 ↗
37. 10.1021/jacs.5b0119610.1021/jacs.5b01196 ↗
38. 10.1039/c0cc02363a10.1039/c0cc02363a ↗
39. 10.1038/nrmicro161510.1038/nrmicro1615 ↗
40. 10.1039/c3cc38693g10.1039/c3cc38693g ↗
41. 10.1016/j.jconrel.2016.03.02210.1016/j.jconrel.2016.03.022 ↗
42. 10.1021/acsami.5b1029510.1021/acsami.5b10295 ↗
43. 10.1016/S0092-8674(00)81383-510.1016/S0092-8674(00)81383-5 ↗