journal article·2020-03-18

Nanoplasmonic On-Chip PCR for Rapid Precision Molecular Diagnostics

Youngseop Lee, Byoung-Hoon Kang, Minhee Kang, Doo Ryeon Chung, Gwan-Su Yi, Luke P. Lee, Ki-Hun Jeong

ACS Applied Materials & Interfaces· Volume 12· Issue 11
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Abstract

Emerging molecular diagnosis requires ultrafast polymerase chain reaction (PCR) on chip for rapid precise detection of infectious diseases in the point-of-care test. Here, we report nanoplasmonic on-chip PCR for rapid precision molecular diagnostics. The nanoplasmonic pillar arrays (NPA) comprise gold nanoislands on the top and sidewall of large-scale glass nanopillar arrays. The nanoplasmonic pillars enhance light absorption of a white light-emitting diode (LED) over the whole visible range due to strong electromagnetic hotspots between the nanoislands. As a result, they effectively induce photothermal heating for ultrafast PCR thermal cycling. The temperature profile of NPA exhibits 30 cycles between 98 and 60 °C for a total of 3 min and 30 s during the cyclic excitation of white LED light. The experimental results also demonstrate the rapid DNA amplification of both 0.1 ng μL–1 of λ-DNA in 20 thermal cycles and 0.1 ng μL–1 of complementary DNA of Middle East respiratory syndrome coronavirus in 30 thermal cycles using a conventional PCR volume of 15 μL. This nanoplasmonic PCR technique provides a new opportunity for rapid precision molecular diagnostics.
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Research context

OpenAlex topics: Biosensors and Analytical Detection · Innovative Microfluidic and Catalytic Techniques Innovation · Microfluidic and Capillary Electrophoresis Applications

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Department of Bio and Brain Engineering, Korea Advanced Institute of Science and Technology (KAIST), 291 Daehak-ro, Yuseong-gu, Daejeon 34141, Republic of Korea

KAIST Institute for Health Science and Technology (KIHST), Korea Advanced Institute of Science and Technology (KAIST), 291 Daehak-ro, Yuseong-gu, Daejeon 34141, Republic of Korea

Biomedical Engineering Research Center, Smart Healthcare Research Institute, Samsung Medical Center, 81 Irwon-ro, Gangnam-gu, Seoul 06351, Republic of Korea

Department of Medical Device Management and Research, SAIHST (Samsung Advanced Institute for Health Sciences & Technology), Sungkyunkwan University, 81 Irwon-ro, Gangnam-gu, Seoul 06351, Republic of Korea

Division of Infectious Disease, Department of Internal Medicine, Samsung Medical Center, Sungkyunkwan University School of Medicine, 81 Irwon-ro, Gangnam-gu, Seoul 06351, Republic of Korea

Center for Infection Prevention and Control, Samsung Medical Center, 81 Irwon-ro, Gangnam-gu, Seoul 06351, Republic of Korea

Asia Pacific Foundation for infectious Diseases (APFID), 81 Irwon-ro, Gangnam-gu, Seoul 06351, Republic of Korea

Department of Bioengineering, University of California, Berkeley, California 94720, United States

Berkeley Sensor and Actuator Center, University of California, Berkeley, California 94720, United States

Department of Electrical Engineering and Computer Science, University of California, Berkeley, California 94720, United States

Biophysics Graduate Program, University of California, Berkeley, California 94720, United States

Funding

Ministry of Science and ICT

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