Quenching Electrochemiluminescence Immunosensor Based on Resonance Energy Transfer between Ruthenium (II) Complex Incorporated in the UiO-67 Metal–Organic Framework and Gold Nanoparticles for Insulin Detection

This work describes a sandwich-type electrochemiluminescence (ECL) strategy for insulin detection by using Ru(bpy)₃²⁺ as the luminophore which was encapsulated in the UiO-67 metal-organic framework (UiO-67/Ru(bpy)₃²⁺). Because UiO-67 possesses the characteristics of large specific surface area and porosity, more Ru(bpy)₃²⁺ could be loaded onto its surface and holes, thus greatly improving the ECL efficiency. Furthermore, the ECL resonance energy transfer (ECL-RET) could occur between UiO-67/Ru(bpy)₃²⁺ (ECL donor) and Au@SiO₂ nanoparticles (ECL acceptor), resulting in a conspicuously decreased ECL response. The ECL spectrum of UiO-67/Ru(bpy)₃²⁺ which exhibited strong ECL intensity has suitable spectral overlap with the absorption spectrum of Au@SiO₂, which further proved the occurrence of the ECL-RET action. The ECL intensity decreased with the increase of the concentration of insulin. In addition, the sandwich-type ECL immunosensor was applied to insulin detection, and the ECL decrease efficiency was found to be logarithmically related to the concentration of the insulin antigen in the range of 0.0025 to 50 ng mL^-1 with the limit of detection of 0.001 ng mL^-1. Meanwhile, this work provides an important reference for the application of metal-organic frameworks in the ECL and ECL-RET study and also exhibits potential capability in the detection of other hormones.