journal article·2020-05-06

3D Bioprinting of Carbohydrazide-Modified Gelatin into Microparticle-Suspended Oxidized Alginate for the Fabrication of Complex-Shaped Tissue Constructs

Dong Nyoung Heo, Mecit Altan Alioglu, Yang Wu, Veli Ozbolat, Bugra Ayan, Madhuri Dey, Youngnam Kang, Ibrahim T. Ozbolat

ACS Applied Materials & Interfaces· Volume 12· Issue 18
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Abstract

Extrusion-based bioprinting of hydrogels in a granular secondary gel enables the fabrication of cell-laden three-dimensional (3D) constructs in an anatomically accurate manner, which is challenging using conventional extrusion-based bioprinting processes. In this study, carbohydrazide-modified gelatin (Gel-CDH) was synthesized and deposited into a new multifunctional support bath consisting of gelatin microparticles suspended in an oxidized alginate (OAlg) solution. During extrusion, Gel-CDH and OAlg were rapidly cross-linked because of the Schiff base formation between aldehyde groups of OAlg and amino groups of Gel-CDH, which has not been demonstrated in the domain of 3D bioprinting before. Rheological results indicated that hydrogels with lower OAlg to Gel-CDH ratios possessed superior mechanical rigidity. Different 3D geometrically intricate constructs were successfully created upon the determination of optimal bioprinting parameters. Human mesenchymal stem cells and human umbilical vein endothelial cells were also bioprinted at physiologically relevant cell densities. The presented study has offered a novel strategy for bioprinting of natural polymer-based hydrogels into 3D complex-shaped biomimetic constructs, which eliminated the need for cytotoxic supplements as external cross-linkers or additional cross-linking processes, therefore expanding the availability of bioinks.
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Research context

OpenAlex topics: 3D Printing in Biomedical Research · Innovative Microfluidic and Catalytic Techniques Innovation · Neuroscience and Neural Engineering

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Department of Engineering Science and Mechanics Department, The Pennsylvania State University, University Park, State College, Pennsylvania 16802, United States

The Huck Institutes of the Life Sciences, The Pennsylvania State University, University Park, State College, Pennsylvania 16802, United States

Department of Dental Materials, School of Dentistry, Kyung Hee University, Seoul 02447, Republic of Korea

School of Mechanical Engineering and Automation, Harbin Institute of Technology, Shenzhen 518055, China

Department of Mechanical Engineering, Ceyhan Engineering Faculty, Cukurova University, Adana 01950, Turkey

Chemistry Department, Penn State University, University Park, State College, Pennsylvania 16802, United States

Biomedical Engineering Department, Pennsylvania State University, University Park, State College, Pennsylvania 16802, United States

Materials Research Institute, Pennsylvania State University, University Park, State College, Pennsylvania 16802, United States

Neurosurgery Department, College of Medicine, Pennsylvania State University, Hershey, Pennsylvania 17033, United States

Funding

National Research Foundation of Korea · Osteology Foundation · Division of Civil, Mechanical and Manufacturing Innovation · T?rkiye Bilimsel ve Teknolojik Arastirma Kurumu

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