DataVXACS Applied Materials & InterfacesPaper
journal article·2021-06-02

Core–Shell Structured Antimicrobial Nanofiber Dressings Containing Herbal Extract and Antibiotics Combination for the Prevention of Biofilms and Promotion of Cutaneous Wound Healing

Raghavendra Ramalingam, Chetna Dhand, Venkatesh Mayandi, Chak Ming Leung, Hariharan Ezhilarasu, Sathish Kumar Karuppannan, Praseetha Prasannan, Seow Theng Ong, Nandhini Sunderasan, Ilango Kaliappan, Mohammed Kamruddin, Veluchamy Amutha Barathi, Navin Kumar Verma, Seeram Ramakrishna, Rajamani Lakshminarayanan, Kantha Deivi Arunachalam

ACS Applied Materials & Interfaces· Volume 13· Issue 21
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Source abstract

Abstract

Burn wounds are susceptible to microbial invasion from both resident and exogenous bacteria, which becomes a critical public health issue and causes substantial economic burden. There is a perceived demand to produce new antimicrobial wound dressings that hinder bacterial colonization while accelerating the healing process and hence would provide an improved standard of care for patients. Since ancient times, herbal extracts from medicinally important plants have extensively been used for treating burn injuries. This work reports the utility of electrospun nanofibers containing plant extracts and antibiotics combination as a multifunctional scaffold for treating second-degree burns. First, we determined the various components of plant extracts from Gymnema sylvestre by two different processing methods and their synergism with minocycline antibiotics. Then, we prepared core–shell nanofibrous dressings with poly-ε-caprolactone/gelatin laden with minocycline hydrochloride as a shell and gelatin infused with G. sylvestre extracts (ultrasound-assisted extracts and cold macerated extracts) as the core using coaxial electrospinning. The electrospun nanofibers displayed a smooth, continuous, and bead-free morphology with adequate wettability. The presence of extract components in the core–shell nanofibers resulted in enhanced mechanical properties when compared to pristine mats. The core–shell structures resulted in sustained release of the bioactive components when compared to nanofiber blends. Core–shell nanofiber mats containing plant extracts and antibiotic combinations displayed potent antimicrobial and antibiofilm properties while promoting the spread and proliferation of skin cells when compared to pristine mats. In a porcine model of cutaneous second-degree burns, we showed that wounds treated with the antimicrobial dressing improved re-epithelialization and collagen organization in comparison to untreated wounds.
Source: openalex_inverted_index · Quality: baseline

Research context

NanomaterialsBiomaterials

OpenAlex topics: Wound Healing and Treatments · Electrospun Nanofibers in Biomedical Applications · Planarian Biology and Electrostimulation

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Center for Environmental Nuclear Research, SRM Institute of Science and Technology, Kattankulathur, Chengalpattu, Tamil Nadu 603203, India

Department of Physics and Nanotechnology, SRM Institute of Science and Technology, Kattankulathur, Chengalpattu, Tamil Nadu 603203, India

Center for Nanofibers and Nanotechnology, Department of Mechanical Engineering, Faculty of Engineering, 2 Engineering Drive 3, National University of Singapore, 117576, Singapore

Ocular Infections & Anti-Infectives Research Group, Singapore Eye Research Institute, The Academia, 20 College Road, Discovery Tower, 169856, Singapore

Industrial Waste Utilization, Nano and Biomaterials, CSIR-Advanced Materials and Processes Research Institute (CSIR-AMPRI), Bhopal, Madhya Pradesh 462026, India

School of Biological Sciences, Nanyang Technological University Singapore, 60 Nanyang Drive, Singapore 637551, Singapore

Department of Biomedical Engineering, National University of Singapore, 117581, Singapore

NUS Graduate School for Integrative Sciences & Engineering, National University of Singapore, 117456, Singapore

Lee Kong Chian School of Medicine, Nanyang Technological University Singapore, Clinical Sciences Building, 11 Mandalay Road, 308232, Singapore

Interdiscipilinary Institute of Indian System of Medicine, SRM Institute of Science and Technology, Kattankulathur, Chengalpattu (Dt), Tamil Nadu 603203, India

Department of Pharmaceutical Chemistry, SRM College of Pharmacy, SRM Institute of Science and Technology, Kattankulathur, Chengalpattu (Dt), Tamil Nadu 603203, India

Materials Physics Division, Material Science Group, Indira Gandhi Centre for Atomic Research, Kalpakkam, Tamilnadu 603102, India

Ophthalmology and Visual Sciences Academic Clinical Program, Duke-NUS Graduate Medical School, 169857, Singapore

Department of Ophthalmology, Yong Loo Lin School of Medicine, National University of Singapore, 1E Kent Ridge Road, NUHS Tower Block, Level 7, 119228, Singapore

Skin Research Institute of Singapore, Clinical Sciences Building, 11 Mandalay Road, 308232, Singapore

Department of Pharmacy, National University of Singapore, 18 Science Drive, 117543, Singapore

Funding

Agency for Science, Technology and Research · National Medical Research Council · UGC-DAE Consortium for Scientific Research · Singapore National Eye Centre · Lee Kong Chian School of Medicine, Nanyang Technological University Singapore

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