DataVXACS Applied Materials & InterfacesPaper
journal article·2017-03-08

Probing Cellular Molecules with PolyA-Based Engineered Aptamer Nanobeacon

Lizhen Chen, Jie Chao, Xiangmeng Qu, Hongbo Zhang, Dan Zhu, Shao Su, Ali Aldalbahi, Lianhui Wang, Hao Pei

ACS Applied Materials & Interfaces· Volume 9· Issue 9
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Source abstract

Abstract

Adenosine triphosphate (ATP) is a central metabolite that is of critical importance in many cellular processes. The development of sensitive and selective methods for the detection of ATP level in vivo is crucial in diagnostic and theranostic applications. In this work, we have developed a polyA-based aptamer nanobeacon (PAaptNB) with improved efficiency and speed of ATP analysis. We found that the dissociation constants and competitive binding kinetics of the PAaptNB could be programmably regulated by adjusting the polyA length. When the polyA length reached to 30 bases, a 10 μM detection limit for ATP assay with PAaptNB can be achieved (∼10-fold improvement compared with the conventional thiol-based aptamer nanobeacon). The feasibility of the PAaptNB for in vivo assay was further demonstrated by imaging intracellular ATP molecules. This study provides a new strategy to construct high-efficiency and high-speed biosensors for cellular molecules analysis, which holds great potential in bioanalysis and theranostic applications.
Source: openalex_inverted_index · Quality: baseline

Research context

Nanomaterials

OpenAlex topics: Advanced biosensing and bioanalysis techniques · Molecular Junctions and Nanostructures · Nanopore and Nanochannel Transport Studies

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Shanghai Key Laboratory of Green Chemistry and Chemical Processes, School of Chemistry and Molecular Engineering, East China Normal University, 500 Dongchuan Road, Shanghai 200241, PR China

Institute of Advanced Materials, Nanjing University of Posts and Telecommunications, Nanjing 210023, PR China

Division of Pharmaceutical Chemistry and Technology, Faculty of Pharmacy, University of Helsinki, FI-00014 Helsinki, Finland

Chemistry Department, King Saud University, Riyadh 11451, Saudi Arabia

Funding

East China Normal University · Ministry of Science and Technology of the People's Republic of China · China Postdoctoral Science Foundation · Science and Technology Commission of Shanghai Municipality · National Natural Science Foundation of China · Deanship of Scientific Research, King Saud University

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