journal article·2023-05-23

Fully Printed Low-Voltage Field-Effect Transistor Biosensor Array for One-Drop Detection of Shewanella onedensis MR-1 Bacteria

Qingqing Sun, Chao Ma, Weipeng Li, Xiaomeng Li, Kenji Sakamoto, Xuying Liu, Akihiro Okamoto, Takeo Minari

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Abstract

Fast and simple bacterial detection using an in situ measurement technique is indispensable for medical diagnosis, public hygiene, and environmental monitoring. However, current bacterial detection techniques still require large measurement apparatus with complicated analysis. In this work, we propose the use of a full printing process for the fabrication of field-effect-transistor (FET) biosensors using π-junction gold nanoparticle (AuNP) ink and semiconducting single-wall carbon nanotube (sc-SWCNT) ink, which show a low voltage (<1 V) and high field-effect mobility (70 cm 2 V –1 s –1 ), allowing for highly efficient and accurate bacterial detection from a minimal size of the target analyte droplet. This method provides a fully printed sensing platform for the determination of Shewanella onedensis MR-1 bacteria with a linear range of 10–1000 CFU in one drop of bacterial suspension (0.1 μL) (i.e., 10 5 to 10 7 CFU/mL), which is promising for the practical application of low-cost and disposable biosensors, especially in the regions with restricted medical conditions.
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Research context

OpenAlex topics: Biosensors and Analytical Detection · Advanced biosensing and bioanalysis techniques · Microfluidic and Capillary Electrophoresis Applications

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

School of Materials Science and Engineering, Zhengzhou Key Laboratory of Flexible Electronic Materials and Thin-Film Technologies, Zhengzhou 450001, China

Printed Electronics Group, Research Center for Functional Materials, National Institute for Materials Science (NIMS), Tsukuba, Ibaraki 305-0044, Japan

International Center for Materials Nanoarchitectonics, National Institute for Materials Science, 1-1 Namiki, Tsukuba, Ibaraki 305-0044, Japan

Department of Medicinal and Applied Chemistry, Kaohsiung Medical University, Kaohsiung 807, Taiwan

School of Mechanics and Safety Engineering, National Center for International Joint Research of Micro-Nano Moulding Technology, Zhengzhou University, Zhengzhou 450001, China

Funding

Japan Society for the Promotion of Science · National Natural Science Foundation of China

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