Hydrophobic pp-HMDSO Coating for Three-Dimensional Cell Culture
Source abstract
Abstract
The interaction of biomaterial surface with cells is a pivotal factor in tissue engineering and three-dimensional (3D) modeling. This paper presents an approach to modifying polystyrene surface by plasma-enhanced chemical vapor deposition of thin plasma-polymerized hexamethyldisiloxane (pp-HMDSO) films to enhance biocompatibility and stimulate the formation of 3D cellular structures. The coatings were characterized by SEM, EDS, XPS, FTIR, AFM, contact angle measurements, and surface free energy (SFE) analysis. A hydrophobic surface initiates 3D structure formation by ensuring uniform cell repulsion and stimulating intercellular interactions. Biological evaluation was performed on U-87 MG (glioblastoma) and HMC3 (microglia) cell lines. For U-87 MG, the pp-HMDSO layer proved critical: cell death and atypical adhesion occurred on untreated plastic, whereas stable spheroids formed on the modified surface. HMC3 cells formed small spheroids even on unmodified surfaces, but on pp-HMDSO coatings, the process was more intense and the structures more uniform due to surface hydrophobicity. These results demonstrate the potential of plasma-polymerized HMDSO films as a scalable platform for creating biomaterials with controlled properties for 3D culturing.
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Research context
OpenAlex topics: Surface Modification and Superhydrophobicity · Polymer Surface Interaction Studies · Bone Tissue Engineering Materials
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Institute of Chemical Biology and Fundamental Medicine Siberian Branch of the Russian Academy of Sciences, 8 Acad. Lavrentiev Ave., Novosibirsk 630090, Russia
Scientific Center of Genetics and Life Sciences, Sirius University of Science and Technology, 1 Olimpiysky Ave., Sirius Federal Territory, Sirius 354340, Russia
Nikolaev Institute of Inorganic Chemistry Siberian Branch of the Russian Academy of Sciences, 3 Acad. Lavrentiev Ave., Novosibirsk 630090, Russia
Funding
STATE PROGRAM OF THE “SIRIUS” FEDERAL TERRITORY, “Scientific and technological development of the “Sirius” Federal Territory”
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