Comparative Effects of Experimental Bioactive Glasses on Dentin Permeability and Tubule Occlusion In Vitro
Source abstract
Abstract
This study aimed to evaluate the effects of experimental bioactive glass solutions on dentinal fluid flow and hydraulic conductance in vitro. Dentin blocks of 50 bovine incisors were divided into 5 groups according to the desensitizing agent used (n = 10): Group 1—control (no treatment); Group 2—Bioglass® 45S5; Group 3—Bioglass® F18; Group 4—Biosilicate gel; Group 5—Desensibilize Nano P (FGM). Treatments were applied for 20 min daily over 15 days. The specimens were subjected to a citric acid challenge to simulate oral demineralizing conditions. Dentinal fluid flow and hydraulic conductance were evaluated before and after the desensitizing procedures and the acidic challenge. Scanning electron microscopy (SEM) provided qualitative dentin surface analysis. Dentinal fluid flow and hydraulic conductance data were analyzed by two-way repeated measures ANOVA and Tukey’s test (α = 0.05). The control group showed the highest dentinal fluid flow and hydraulic conductance values after the acid challenge (p < 0.05). After product application, Bioglass® 45S5 and Bioglass® F18 showed the lowest dentinal fluid flow (0.463 ± 0.124 Lp and 0.549 ± 0.239 Lp, respectively) and hydraulic conductance values (0.025 ± 0.007 Lp and 0.026 ± 0.007 Lp, respectively), differing significantly from Biosilicate Gel and Desensibilize Nano P (p < 0.0001). Biosilicate Gel and Desensibilize Nano P showed higher dentinal fluid flow (0.864 ± 0.180 Lp and 0.840 ± 0.173 Lp, respectively) and hydraulic conductance values (0.047 ± 0.010 Lp and 0.046 ± 0.009 Lp, respectively) after application (p < 0.0001). After the acid challenge, no significant differences were observed among the groups (p > 0.05), although all experimental groups showed numerically lower values than the control group. Bioactive glass-based desensitizing agents reduced dentinal fluid flow and hydraulic conductance, suggesting dentinal tubule occlusion. Bioglass® 45S5 and Bioglass® F18 showed the most stable performance, maintaining lower values even after the acid challenge, suggesting potential for the management of dentin hypersensitivity.
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Research context
OpenAlex topics: Dental Erosion and Treatment · Endodontics and Root Canal Treatments · Dental materials and restorations
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Department of Dental Materials and Prosthodontics, Aracatuba School of Dentistry, São Paulo State University, Aracatuba 16015-050, SP, Brazil
Department of Restorative Dentistry, Focus Educational Group, Teresina 64052-280, PI, Brazil
Faculty of Dentistry, University of Toronto, Toronto, ON M5G 1X3, Canada
Department of Restorative Dentistry and Endodontics, Aracatuba School of Dentistry, São Paulo State University, Aracatuba 16015-050, SP, Brazil
Department of Restorative Dentistry, School of Dentistry, Federal University of Minas Gerais (UFMG), Belo Horizonte 31270-901, MG, Brazil
Vitreous Materials Laboratory (LaMaV), Department of Materials Engineering, Federal University of Sao Carlos (UFSCar), Sao Carlos 13565-905, SP, Brazil
References (55)
Show source-provided references
1. Comparison of in vitro dentinal tubule occluding efficacy of two different methods using a nano-scaled bioactive glass-containing desensitising agent10.1016/j.jdent.2017.03.001 ↗
2. Evaluation of Three Methods for the Treatment of Dentin Hypersensitivity: A Randomised Clinical Trial10.1016/j.identj.2024.03.013 ↗
3. Effects of different desensitizing treatments on root dentin permeability10.1590/1807-3107BOR-2016.vol30.0111 ↗
4. Laser therapy for dentinal hypersensitivity
5. Movement of dentinal and pulpal fluid caused by clinical procedures10.1177/00220345680470050201 ↗
6. A clinical, randomized, controlled study on the use of desensitizing agents during tooth bleaching10.1016/j.jdent.2015.07.002 ↗
7. Dentin-Desensitizing Biomaterials10.1016/j.cclet.2022.108060 ↗
8. Dionysopoulos, D., Gerasimidou, O., and Beltes, C. (2023). Dentin hypersensitivity: Etiology, diagnosis and contemporary therapeutic approaches—A review in literature. Appl. Sci., 13.10.3390/app132111632 ↗
9. The Effectiveness of Remineralizing Agents on Dentinal Permeability10.1155/2018/4072815 ↗
10. An update on dentinal hypersensitivity-aetiology to management: A review10.14260/jemds/2021/667 ↗
11. In vitro efficacy of a novel potassium oxalate hydrogel for dentin hypersensitivity10.1111/eos.12334 ↗
12. Successful management of dentin hypersensitivity: A narrative review10.17219/dmp/143354 ↗
13. Laser therapy versus topical desensitising agents in the management of dentine hypersensitivity: A meta-analysis10.1111/odi.13309 ↗
14. Efficacy of GLUMA for the treatment of dentin hypersensitivity compared to lasers: A systematic review10.5005/jp-journals-10024-3420 ↗
15. Wang, T.D., Yang, L., and Wang, H.L. (2015). Use of poly (amidoamine) dendrimer for dentinal tubule occlusion: A preliminary study. PLoS ONE, 10.10.1371/journal.pone.0124735 ↗
16. West, Efficacy of desensitizing dentifrices to occlude dentinal tubules10.1111/j.1600-0722.2011.00872.x ↗
17. Effect of a 3% potassium oxalate topical application on dentinal hypersensitivity after subgingival scaling and root planning10.1902/jop.2004.75.11.1461 ↗
18. Multi-component bioactive glasses of varying fluoride content for treating dentin hypersensitivity10.1016/j.dental.2011.11.021 ↗
19. Desensitizing efficacy of a universal dentin adhesive containing mesoporous bioactive glass on dentin hypersensitivity: A randomized clinical trial with a split-mouth model10.1038/s41598-024-64404-x ↗
20. A novel mesoporous biomaterial for treating dentin hypersensitivity10.1177/0022034509357148 ↗
21. Al-Haddad, A., Alarami, N., Alshammari, A.F., and Madfa, A.A. (2025). Efficacy of bioactive glass-based desensitizer compared to other desensitizing agents or techniques in dentin hypersensitivity: A systematic review. BMC Oral Health, 25.10.1186/s12903-025-06288-5 ↗
22. Bioactive glass and high-intensity lasers as a promising treatment for dentin hypersensitivity: An in vitro study10.1002/jbm.b.34446 ↗
23. The effects of strontium-doped bioactive glass and fluoride on hydroxyapatite crystallization10.1016/j.jdent.2021.103581 ↗
24. Choi, Y.J., Bae, M.K., Kim, Y.I., Park, J.K., and Son, S.A. (2020). Effects of microsurface structure of bioactive nanoparticles on dentinal tubules as a dentin desensitizer. PLoS ONE, 15.10.1371/journal.pone.0237726 ↗
25. Reducing dentine hypersensitivity with nano-hydroxyapatite toothpaste: A double-blind randomized controlled trial10.1007/s00784-017-2113-3 ↗
26. Multifunctional Bioactive Glass and Glass-Ceramic Biomaterials with Antibacterial Properties for Repair and Regeneration of Bone Tissue10.1016/j.actbio.2017.06.046 ↗
27. Bioactive Glasses beyond Bone and Teeth: Emerging Applications in Contact with Soft Tissues
28. Regeneration of Dental–Pulp Complex-like Tissue Using Phytic Acid Derived Bioactive Glasses10.1039/C7RA01480E ↗
29. Odontogenic Differentiation and Dentin Formation of Dental Pulp Cells under Nanobioactive Glass Induction10.1016/j.actbio.2014.02.013 ↗
30. The Effect of Bioactive Glasses on Enamel Remineralization: A Systematic Review10.1016/j.jdent.2017.09.007 ↗
31. Alamri, A., Salloot, Z., Alshaia, A., and Ibrahim, M.S. (2020). The Effect of Bioactive Glass-Enhanced Orthodontic Bonding Resins on Prevention of Demineralization: A Systematic Review. Molecules, 25.10.3390/molecules25112495 ↗
32. Review of bioactive glass: From Hench to hybrids10.1016/j.actbio.2012.08.023 ↗
33. Kim, H.J., Jang, J.H., Woo, S.U., Choi, K.K., Kim, S.Y., Ferracane, J.L., Lee, J.H., Choi, D., Choi, S., and Kim, S. (2021). Effect of novel bioactive glass-containing dentin adhesive on the permeability of demineralized dentin. Materials, 14.10.3390/ma14185423 ↗
34. Bioactive glass in tissue engineering10.1016/j.actbio.2011.03.016 ↗
35. Fernandes, H.R., Gaddam, A., Rebelo, A., Brazete, D., Stan, G.E., and Ferreira, J.M.F. (2018). Bioactive Glasses and Glass-Ceramics for Healthcare Applications in Bone Regeneration and Tissue Engineering. Materials, 11.10.3390/ma11122530 ↗
36. Remineralization of human dentin using ultrafine bioactiveglass particles10.1016/j.actbio.2007.04.003 ↗
37. Synthesis of nanobioactive glass and formation of apatite rods to occlude exposed dentine tubules and eliminate hypersensitivity10.1016/j.actbio.2010.02.045 ↗
38. Cytotoxicity of 45S5 bioactive glass paste used for dentine hypersensitivity treatment10.1016/j.jdent.2011.06.003 ↗
39. Effectiveness and acid/tooth brushing resistance of in-office desensitizing treatments—A hydraulic conductance study10.1016/j.archoralbio.2018.09.004 ↗
40. Liu, X.X., Tenenbaum, H.C., Wilder, R.S., Quock, R., Hewlett, E.R., and Ren, Y.F. (2020). Pathogenesis, diagnosis and management of dentin hypersensitivity: An evidence-based overview for dental practitioners. BMC Oral Health, 20.10.1186/s12903-020-01199-z ↗
41. Cytotoxicity of dentine-bonding agents on human pulp cells in vitro10.1046/j.1365-2591.2002.00589.x ↗
42. Mineralization of dentin induced by treatment with bioactive glass S53P4 in vitro10.1080/00016350310008012 ↗
43. Evaluation of Antibacterial Effectiveness of Desensitizers against Oral Bacteria10.1055/s-0039-1697353 ↗
44. Strong effect on dentin after the use of high concentrations of citric acid: An assessment with co-site optical microscopy and ESEM10.1016/j.dental.2008.03.027 ↗
45. Impact of Various Desensitizing Agents on Occlusion of Dentinal Tubules: A Scanning Electron Microscopic Study10.5005/jp-journals-10024-3129 ↗
46. Comparative Evaluation of Two Novel Natural Desensitizing Agents on Dentinal Tubule Occlusion: A Scanning Electron Microscopy Study10.5005/jp-journals-10024-3756 ↗
47. Dentin sealing and antibacterial effects of silver-doped bioactive glass/mesoporous silica nanocomposite: An in vitro study10.1007/s00784-018-2432-z ↗
48. Effect of mesoporous silica nanoparticles on dentinal tubule occlusion: An in vitro study using SEM and image analysis10.4012/dmj.2013-215 ↗
49. Bioactive toothpastes in dentin hypersensitivity treatment: A systematic review10.1016/j.sdentj.2021.04.004 ↗
50. Biovidro 45S5: Um avanço biotecnológico nos materiais restauradores da odontologia10.15600/2238-1236/fol.v25n2p47-55 ↗
51. Campanini, L.A. (2015). Avaliação da Atividade Bactericida do Biovidro F18 e F18 com Prata Para Aplicações Médicas. [Master’s Thesis, Universidade Federal de São Carlos].
52. A novel bioactive glass-ceramic for treating dentin hypersensitivity10.1590/S1806-83242010000400002 ↗
53. Treatment of Dentin Hypersensitivity Using Nano-Hydroxyapatite Pastes: A Randomized Three-Month Clinical Trial10.2341/15-145-C ↗
54. In vitro effect of innovative desensitizing agents on dentin tubule occlusion and erosive wear10.2341/17-284-L ↗
55. Analysis of permeability and biological properties of dentin treated with experimental bioactive glasses10.1016/j.jdent.2021.103719 ↗