Osteogenic and Anti-Inflammatory Effects of Strontium-Loaded Polydopamine on Micro-Arc Oxidized Titanium Surfaces
Source abstract
Abstract
Titanium implants are widely used in orthopedic and dental fields but often face challenges such as insufficient osseointegration and peri-implant inflammation. While Strontium (Sr) possesses potent bioactive properties, achieving its controlled delivery at the implant-tissue interface remains technically challenging. To address this, we engineered a multidimensional composite coating by constructing a micro/nano-porous TiO2 substrate via micro-arc oxidation (MAO), followed by polydopamine (PDA)-assisted Sr immobilization. This integrated architecture significantly enhanced surface hydrophilicity and facilitated high-content Sr loading with sustained release kinetics. Biological evaluations demonstrated that the PDA-mediated interface promoted superior initial adhesion and spreading of bone marrow mesenchymal stem cells (BMSCs), synergizing with released Sr2+ to markedly upregulate core osteogenic markers (Runx2, ALP). Crucially, the functionalized surface actively optimized the immune microenvironment by inducing M1-to-M2 macrophage polarization and comprehensively suppressing RANKL-induced osteoclastogenesis via the downregulation of TRAP and DC-STAMP. By integrating these pro-osteogenic, anti-inflammatory, and anti-resorptive capabilities, this tri-functional system effectively rebalances the bone remodeling microenvironment. Consequently, it provides a robust, universally applicable strategy for enhancing the therapeutic efficacy of next-generation orthopedic and dental implants.
Source: crossref · Quality: baseline
Research context
OpenAlex topics: Polymer Surface Interaction Studies · Bone Tissue Engineering Materials · Orthopaedic implants and arthroplasty
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
College of Stomatology, Shanghai Jiao Tong University, Shanghai 200011, China
National Center for Stomatology, National Clinical Research Center for Oral Diseases, Shanghai 200011, China
Shanghai Key Laboratory of Stomatology, Shanghai 200011, China
Shanghai Research Institute of Stomatology, Shanghai 200011, China
Department of Orthodontics, Shanghai Ninth People’s Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200011, China
Department of Prosthodontics, Shanghai Ninth People’s Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200011, China
Department of Implant Alveolar Surgery, The Stomatological Center of Weifang People’s Hospital, Weifang 261000, China
Department of General Dentistry, Shanghai Ninth People’s Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200011, China
Funding
National Key Research and Development Program of China · National Clinical Research Center Project of Shanghai Ninth People’s Hospital · Science and Technology Aid Program of Xinjiang Uygur Autonomous Region
References (47)
Show source-provided references
1. Titanium alloys for biomedical applications10.1016/S0921-5093(97)00806-X ↗
2. Romanos, G.E., Fischer, G.A., and Delgado-Ruiz, R. (2021). Titanium Wear of Dental Implants from Placement, under Loading and Maintenance Protocols. Int. J. Mol. Sci., 22.10.3390/ijms22031067 ↗
3. Di Tinco, R., Bertani, G., Pisciotta, A., Bertoni, L., Bertacchini, J., Colombari, B., Conserva, E., Blasi, E., Consolo, U., and Carnevale, G. (2021). Evaluation of Antimicrobial Effect of Air-Polishing Treatments and Their Influence on Human Dental Pulp Stem Cells Seeded on Titanium Disks. Int. J. Mol. Sci., 22.10.3390/ijms22020865 ↗
4. The Effects of Strontium-Substituted Bioactive Glasses on Osteoblasts and Osteoclasts in Vitro10.1016/j.biomaterials.2010.01.121 ↗
5. Strontium Signaling: Molecular Mechanisms and Therapeutic Implications in Osteoporosis10.1016/j.pharmthera.2012.07.009 ↗
6. Strontium-Doped Bioactive Glass Nanoparticles in Osteogenic Commitment10.1021/acsami.8b06154 ↗
7. Osteoprotegerin deficiency attenuates strontium-mediated inhibition of osteoclastogenesis and bone resorption10.1002/jbmr.325 ↗
8. Strontium substitution in apatitic CaP cements effectively attenuates osteoclastic resorption but does not inhibit osteoclastogenesis10.1016/j.actbio.2016.04.016 ↗
9. Strontium modified calcium phosphate cements—Approaches towards targeted stimulation of bone turnover10.1039/C5TB00654F ↗
10. TiO2 bioactive implant surfaces doped with specific amount of Sr modulate mineralization10.1016/j.msec.2020.111735 ↗
11. High proportion strontium-doped micro-arc oxidation coatings enhance early osseointegration of titanium in osteoporosis by anti-oxidative stress pathway
12. Functionalization of Ti-40Nb implant material with strontium by reactive sputtering
13. Carbonate Apatite Honeycomb Scaffold-Based Drug Delivery System for Repairing Osteoporotic Bone Defects10.1021/acsami.4c08047 ↗
14. Synthesis and Properties of Hydroxyapatite-Containing Porous Titania Coating on Ultrafine-Grained Titanium by Micro-Arc Oxidation10.1016/j.actbio.2009.12.053 ↗
15. Assessment of osteoinduction using a porous hydroxyapatite coating prepared by micro-arc oxidation on a new titanium alloy10.1016/j.ijsu.2015.08.030 ↗
16. Mussel-inspired tissue-adhesive hydrogel based on the polydopamine-chondroitin sulfate complex for growth-factor-free cartilage regeneration10.1021/acsami.8b05314 ↗
17. Mussel-inspired polydopamine coating: A general strategy to enhance osteogenic differentiation and osseointegration for diverse implants10.1021/acsami.8b21558 ↗
18. Polydopamine-coated 3D-printed β-tricalcium phosphate scaffolds to promote the adhesion and osteogenesis of BMSCs for bone-defect repair: mRNA transcriptomic sequencing analysis10.1039/D2TB02280J ↗
19. Polydopamine-assisted immobilization of copper ions onto hemodialysis membranes for antimicrobial applications10.1021/acsabm.8b00106 ↗
20. Mussel-inspired polydopamine composite mesoporous bioactive glass nanoparticles: An exploration of potential metal-ion loading platform and in vitro bioactivity10.1021/acsami.3c03680 ↗
21. Metal Ions in Polydopamine Coatings Enhance Polymer-Metal Adhesion10.1021/acsapm.4c03551 ↗
22. Inhibition of Inflammatory Response and Promotion of Osteogenic Activity of Zinc-Doped Micro-Arc Titanium Oxide Coatings10.1021/acsomega.2c00579 ↗
23. Preparation of Cerium-Containing Nanoporous Titanium Coatings for Osseointegration and Their Promotion of Osteogenic Differentiation and Anti-Inflammatory Properties10.1016/j.mtchem.2023.101821 ↗
24. The Synergistic Effect of Hierarchical Micro/Nano-Topography and Bioactive Ions for Enhanced Osseointegration10.1016/j.biomaterials.2013.01.008 ↗
25. Accelerated and Enhanced Osteointegration of MAO-Treated Implants: Histological and Histomorphometric Evaluation in a Rabbit Model10.1038/s41368-018-0008-z ↗
26. Effect of microarc oxidation-treated Ti6Al4V scaffold following low-intensity pulsed ultrasound stimulation on osteogenic cells in vitro
27. Surface modification of titanium implants with Mg-containing coatings to promote osseointegration10.1016/j.actbio.2023.07.048 ↗
28. Atomic layer deposition coating of TiO2 nano-thin films on magnesium-zinc alloys to enhance cytocompatibility for bioresorbable vascular stents10.2147/IJN.S199093 ↗
29. Intracellular co-delivery of Sr ion and phenamil drug through mesoporous bioglass nanocarriers synergizes BMP signaling and tissue mineralization10.1016/j.actbio.2017.07.021 ↗
30. PEO/Polymer Hybrid Coatings on Magnesium Alloy to Improve Biodegradation and Biocompatibility Properties10.1016/j.surfin.2022.102495 ↗
31. Metal-Polydopamine Coordinated Coatings on Titanium Surface: Enhancing Corrosion Resistance and Biological Property10.1039/D5RA00301F ↗
32. Fabrication of a ZnO/Polydopamine/ε-Polylysine Coating with Good Corrosion Resistance and a Joint Antibacterial Pathway on the Surface of Medical Stainless Steel10.1021/acsbiomaterials.5c00087 ↗
33. Chitosan Nanofiber Scaffold Improves Bone Healing via Stimulating Trabecular Bone Production Due to Upregulation of the Runx2/Osteocalcin/Alkaline Phosphatase Signaling Pathway
34. Komori, T. (2019). Regulation of Proliferation, Differentiation and Functions of Osteoblasts by Runx2. Int. J. Mol. Sci., 20.10.3390/ijms20071694 ↗
35. Effect of BMP-2 and/or BMP-9 on Preosteoblasts Attached to Polycaprolactone Functionalized by Adhesive Peptides Derived from Bone Sialoprotein10.1016/j.biomaterials.2012.10.066 ↗
36. FAK and BMP-9 Synergistically Trigger Osteogenic Differentiation and Bone Formation of Adipose Derived Stem Cells Through Enhancing Wnt-β-Catenin Signaling
37. Bioactive Porous Composite Implant Guides Mesenchymal Stem Cell Differentiation and Migration to Accelerate Bone Reconstruction10.2147/IJN.S479893 ↗
38. Nanorod Diameter Modulated Osteogenic Activity of Hierarchical Micropore/Nanorod-Patterned Coatings via a Wnt/β-Catenin Pathway10.1016/j.nano.2018.04.006 ↗
39. Sr-Doped Nanowire Modification of Ca-Si-Based Coatings for Improved Osteogenic Activities and Reduced Inflammatory Reactions10.1088/1361-6528/aaa2b4 ↗
40. Modulation of Macrophage Polarization by Secondary Cross-Linked Hyaluronan-Dopamine Hydrogels10.1016/j.ijbiomac.2024.132417 ↗
41. Bioactive strontium ions/ginsenoside Rg1–incorporated biodegradable silk fibroin-gelatin scaffold promoted challenging osteoporotic bone regeneration10.1016/j.mtbio.2021.100141 ↗
42. Strontium-Doped Hydroxyapatite Coating Improves Osteo/Angiogenesis for Ameliorative Graft-Bone Integration via the Macrophage-Derived Cytokines-Mediated Integrin Signal Pathway10.1021/acsami.3c14904 ↗
43. Strontium Inhibits Osteoclastogenesis by Enhancing LRP6 and β-Catenin-Mediated OPG Targeted by MiR-181d-5p10.1007/s12079-018-0478-y ↗
44. Strontium Inhibits Titanium Particle-Induced Osteoclast Activation and Chronic Inflammation via Suppression of NF-κB Pathway10.1038/srep36251 ↗
45. Strontium Ranelate Reduces the Risk of Vertebral Fracture in Young Postmenopausal Women with Severe Osteoporosis10.1136/ard.2008.094516 ↗
46. Strontium Ranelate: A Review of Its Use in the Treatment of Postmenopausal Osteoporosis10.2165/10481900-000000000-00000 ↗
47. Molecular Mechanisms of Strontium in Regulating Bone Metabolism and Its Therapeutic Potential in Osteoporosis