LHRH-Conjugated Magnetite Nanoparticles and Nanorods as Magnetic Resonance Imaging Contrast Agents for Targeting Triple-Negative Breast Cancer
Source abstract
Abstract
This paper presents the results of an experimental study on the effects of magnetite nanoparticle (MNP) shape on magnetic resonance imaging (MRI) of triple-negative breast cancer (TNBC) xenograft tissues. T2-weighted MRI scans using spherical shaped MNPs as contrast agents are compared to MRI scans done with nanorod-shaped MNPs as contrast agents. The MNPs were first coated with polyethylene glycol (PEG) and subsequently conjugated to luteinizing hormone-releasing hormone (LHRH) to specifically target LHRH receptors, which are present at high levels on the surfaces of xenograft TNBC cells/tissues. After 3 weeks of tumor growth in nude immunocompromised mice, LHRH-conjugated (functionalized) and unconjugated (non-functionalized) MNPs were injected into the immunocompromised mice. Four types of MNPs were used: non-functionalized nanorod-shaped MNPs (BMNR); LHRH-conjugated nanorod-shaped MNPs (LCMNR); non-functionalized spherical-shaped MNPs (BSSMNP); and LHRH-conjugated spherical-shaped MNPs (LCSSMNP). T2-weighted magnetic resonance imaging (MRI) scans were obtained from the mice before the injection of the MNPs and two hours after the injection of the MNPs. The results show that using nanorod-shaped LHRH-conjugate MNPs as contrast agents yielded higher-resolution T2-weighted MRI scans of TNBC tumors compared to those from spherical-shaped MNPs. The implications of the results are discussed in relation to potential applications of functionalized MNPs in MRI for TNBC diagnosis.
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Research context
OpenAlex topics: Nanoparticle-Based Drug Delivery · Lanthanide and Transition Metal Complexes · Characterization and Applications of Magnetic Nanoparticles
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Department of Materials Science and Engineering, African University of Science and Technology, Km 10 Airport Road, Galadimawa, Abuja 900107, Nigeria
Department of Mechanical and Materials Engineering, Worcester Polytechnic Institute, 100 Institute Road, Worcester, MA 01609, USA
Department of Biomedical Engineering, Worcester Polytechnic Institute, Gateway Park Life Sciences and Bioengineering Centre, Worcester, MA 01605, USA
Department of Engineering, Boston College, 245 Beacon Street, Chestnut Hill, MA 02467, USA
Department of Mechanical Engineering, Academic City University, Property #79-30, Agbogba, Accra GE-189-5224, Ghana
Department of Biotechnology, Ebonyi State University, Enugu-Abakaliki Road, Abakaliki 480214, Nigeria
Department of Biomedical Engineering, College of Engineering, Afe Babalola University, Ado Ekiti, Km 8.5 Afe Babalola Way, Ado Ekiti 360102, Nigeria
Department of Biotechnology, Sheda Science and Technology Complex, Abuja 904105, Nigeria
Department of Mechanical Engineering, State University of New York (SUNY Poly) Polytechnic Institute, 100 Seymour Street, Utica, NY 13502, USA
Funding
World Bank Centers of Excellence · Worcester Polytechnic Institute Global Impact Fund · State of New York Polytechnic Institute
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