Electrochemically embeded enzyme in polypyrrole film and its application in biosensor

Lactate dehydrogenase (LDH) was successfully immobilized into conductive polypyrrole (PPY) film by electrochemical embedding, obtaining PPY-LDH /Au electrode with bioactive and electroactive. The enzyme electrode can be used to test pyruvate concentration as a biosensor. An electroactive PPY-LDH-NADH/Au composite electrode also was constructed by entraping LDH and coenzyme NADH into conducting polypyrrole film during the electropolymerazation. A linear working curve of this electrode was tested making it as a pyruvate biosensor.