The investigation of the hydroxyl activity ability on Ti-O film surface with different structure and its influence to biocompatibility

In this paper,the Titanium oxide films(TiO_2)with three kind of different crystal structure were syn- thesized by unbalanced magnetron sputtering system.The crystal structures of films were characterized by the X-ray diffraction(XRD).The TiO_2 film surface was then treated by alkali solution to obtain hydroxyl functional group.The hydroxyl functional group was detected by fourier transform infrared spectrometer(FTIR)and X- ray photoelectron spectroscopy(XPS)respectively.For evaluating the influence of surface hydroxyl functional group on cell compatibility and blood compatibility of the treated and untreated TiO_2 films surface,the in vitro endothelial cell adhesion behavior and platelet adhesion were investigated respectively.The results showed that, compared to the futile TiO_2 film and the anatase TiO_2 film,the TiO_2 film mixed with anatase and rutile structure possesses the best hydroxyl activity ability.The endothelial cell and platelet adhesion investigation demonstrate that,compared to the untreated TiO_2 films,the hydroxyl surface of treated TiO_2 films has better cell compatibili- ty and blood compatibility due to the more cultured endothelial cells and less adhered platelets onto its surface. This result indicates that the hydroxyl functional group can promote the growth and proliferation of endothelial cells and decrease the platelet adhesion.Furthermore,the work discusses the relationship between the TiO_2 film structure and its hydroxyl ability.