The synthesize and uptake study of quaternized chitosan nano-sized fluorescence probes

In this study,fluorescein isothiocyanate(FITC) was grafted on trimethyl chitosan chloride,and based on contrarily electrostatic interaction we synthesized a new type of nano-sized fluorescence probe TMC-g-FITC/CMC.The size,polydispersity,zeta potential and morphology of the nanoparticles were measured with dynamic laser scattering and transmission electron microscopy.It is found that the nanoparticle was global,the size was between 150-360nm,and the size and zeta potential were adjustable.MTT assay was used to test the cytocixity of the nano-sized fluorescence probes,the uptake of FITC-TMC/CMC nanoparticles by HepG2 cells was observed by confocal laser scanning microscopy.We investigated that the nanoparticles had little toxic to the cells and first aggregated across the cells and then be absorptive by the cell and cell nucleus.