Preparation and blood compatibility of pNSR32/PCL/CS electrospun fibrous vascular scaffolds with small diameter

The composite nano-fiber small diameter vascular scaffold using recombinate spide silk protein with RGD(pNSR32),poly(e-caprolactone)(PCL) and chitosan(CS) was prepared by elecstrospinning.The microstructures of scaffold material were observed using SEM.Additionally,hemolysis ratio,dynamic clotting time,platelet daherence and recalcification time were carried out to evaluate the blood compatibility.These results indicate that scaffolds with 4mm inner diameter and 8cm length possess porosity and 3-diamentions stucture,which benefits for cell attachment,proliferation and migration.The hemolysis rates of the scaffold are less than 5%.The platelet adherence experiments showed there were less blood platelets sticking on the surface of the composite pNSR32/PCL/CS scaffolds,and the platelets were not deformed.The recalcification time of pNSR32/PCL/CS is 293s,which was more 94s than PCL.The pNSR32/PCL/CS composite nano-fiber scaffolds appear to be of fine blood compatibility.The composite nano-fiber scaffolds applly to the small diameter vascular scaffold as a novel biomaterial.