Thero and pH sensitive fluorescent copolymeric sensor for nucleotides

New hydrophobic monomer N-acryl-N′-(quinolin-8-yl)thiourea(AQT) was synthesized and copolymerized with hydrophilic monomer by free radical copolymerization.And N,N-dimethylacrylamide was introduced to adjust the lower critical solution temperature of the resulting copolymers.The fluorescent characteristic of the aqueous solutions of copolymers was investigated in varied pH.The obtained copolymers show thermo-sensitive lower critical solution temperature(LCST) ranging from 32.5 to 37.5℃ with varied ratios of monomers.The interaction of the tercopolymer with adenosine 5′-monophosphate(AMP),adenosine 5′-diphosphate(ADP) and adenosine 5′-trisphosphate(ATP) was described,which demonstrated high selective fluorescent response to ATP.