DataVXSmall MethodsPaper
journal article·2022-07

Well‐Paired‐Seq: A Size‐Exclusion and Locally Quasi‐Static Hydrodynamic Microwell Chip for Single‐Cell RNA‐Seq (Small Methods 7/2022)

Kun Yin, Meijuan Zhao, Li Lin, Yingwen Chen, Shanqing Huang, Chun Zhu, Xuan Liang, Fanghe Lin, Haopai Wei, Huimin Zeng, Zhi Zhu, Jia Song, Chaoyong Yang

Small Methods· Volume 6· Issue 7
Publisher page
Source abstract

Abstract

Inside Back Cover In article number 2200341, Song, Yang, and coworkers developed a high-throughput and cost-effective scRNA-seq method (Well-paired-seq) for revealing the heterogeneity of single cells in complex systems. Based on size exclusion and quasi-static hydrodynamic principles, Well-paired-seq achieves excellent efficiency of cell capture (≈91%), cell and bead pairing (≈82%), cell-free RNA removal, and high throughput (≈105 cells).
Source: openalex_inverted_index · Quality: baseline

Research context

OpenAlex topics: Single-cell and spatial transcriptomics · Innovative Microfluidic and Catalytic Techniques Innovation

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Affiliations

State Key Laboratory of Physical Chemistry of Solid Surfaces The MOE Key Laboratory of Spectrochemical Analysis & Instrumentation Key Laboratory for Chemical Biology of Fujian Province Collaborative Innovation Center of Chemistry for Energy Materials Department of Chemical Biology College of Chemistry and Chemical Engineering Xiamen University Xiamen 361005 P. R. China

Institute of Molecular Medicine State Key Laboratory of Oncogenes and Related Genes Renji Hospital School of Medicine Shanghai Jiao Tong University Shanghai 200120 China

References

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