DataVXSmall MethodsPaper
journal article·2026-06

Programmable Circular Single‐Stranded DNA Acts as Recyclable Anti‐miRNA Nucleotides to Inhibit Colorectal Cancer

Jinghao Wang, Pengfei Zhang, Guang Hu, Danlei Chen, Yonglian Huang, Kexuan Zou, Rui Ye, Xisen Cao, Qianyi Zhang, Yang Li, Songtao Zhou, Boda Guo, Dandan Shao, Rui Liu, Meng Wang, Jianming Wang, Lianxin Liu, Jie Song

Small Methods· Volume 10· Issue 11
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Source abstract

Abstract

ABSTRACT MicroRNA (miRNA) therapy represents an attractive approach for the treatment of colorectal cancer (CRC). However, the efficacy of therapy targeting a single oncomiRNA (oncomiR) is limited, as multiple oncomiRs often act simultaneously in promoting CRC development. In addition, current anti‐miRNA nucleotide (AMiN) design faces challenges, including high synthetic error rate and difficulty in synthesizing long oligonucleotides. Circular single‐stranded DNA (CssDNA), an ancient form of DNA that has gained increasing attention recently, is known for its easy synthesis, high stability, low error rate, and long size potential. Here, we develop a multi‐oncomiR targeting platform with low immunogenicity, high stability and long‐size potential using in vivo M13 phage‐generated CssDNA. This CssDNA acts as a sponge, which attracts and degrades multiple abnormally overexpressed oncomiRs in CRC. The degradation of oncomiRs promotes the expression of tumor suppressor genes (TSGs), inhibiting CRC development. Further, the degradation of oncomiRs from the sponge releases the free CssDNA for second round of action, which results in the efficacy and recyclability of CssDNA. It's visible to spread this novel, sequence customizable, multi‐oncomiR targeting platform using CssDNA as a stable, low‐immunogenic and recyclable AMiN in antagonizing miRNA‐mediated repression of TSG expression for other tumor suppression.
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Research context

OpenAlex topics: Advanced biosensing and bioanalysis techniques · RNA Interference and Gene Delivery · DNA and Nucleic Acid Chemistry

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Department of Chemistry University of Science & Technology of China Hefei Anhui China

Hangzhou Institute of Medicine Chinese Academy of Sciences Hangzhou Zhejiang China

Zhejiang Cancer Hospital Hangzhou Zhejiang China

Institutes of Biomedical Sciences Inner Mongolia University Hohhot China

School of Biomedical Sciences Hunan University Changsha Hunan China

Department of Hepatobiliary Surgery Centre for Leading Medicine and Advanced Technologies of IHM Division of Life Sciences and Medicine The First Affiliated Hospital of USTC University of Science and Technology of China Hefei Anhui China

Department of Respiratory and Critical Care Medicine The Second Affiliated Hospital of Anhui Medical University Hefei Anhui China

Institute of Nano Biomedicine and Engineering Department of Instrument Science and Engineering School of Electronic Information and Electrical Engineering Shanghai Jiao Tong University Shanghai China

School of Life Sciences Tianjin University Tianjin China

Faculty of Health Science University of Macau Avenida Da Universidade Taipa Macau China

Anhui Province Key Laboratory of Hepatopancreatobiliary Surgery Hefei Anhui China

Anhui Provincial Clinical Research Center for Hepatobiliary Diseases Hefei Anhui China

Funding

National Key Research and Development Program of China · Natural Science Foundation of Zhejiang Province

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