DataVXAdvanced Healthcare MaterialsPaper
journal article·2024-03

Ultrahigh‐Efficacy VEGF Neutralization Using Carbonized Nanodonuts: Implications for Intraocular Anti‐Angiogenic Therapy

Hong‐Jyuan Jian, Anisha Anand, Jui‐Yang Lai, Chih‐Ching Huang, David Hui‐Kang Ma, Chi‐Chun Lai, Huan‐Tsung Chang

Advanced Healthcare Materials· Volume 13· Issue 7
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Source abstract

Abstract

Abstract Ocular angiogenesis, associated with diseases such as retinopathy of prematurity and diabetic retinopathy, is a leading cause of irreversible vision loss. Herein, carbon nanodonuts (CNDs) with a donut‐shaped structure are synthesized using sodium alginate (SA) and 1,8‐diaminooctane (DAO) through a one‐step thermal process. The formation of SA/DAO‐CNDs occurs through a crosslinking reaction between SA and DAO, creating amide bonds followed by partial carbonization. In human retinal pigment epithelial cells exposed to H2O2 or lipopolysaccharide, the SA/DAO‐CNDs display a more than fivefold reduction in reactive oxygen species and proinflammatory cytokines, such as IL‐6 and IL‐1β, when compared to carbonized nanomaterials produced exclusively from SA. Furthermore, the CNDs effectively inhibit vascular endothelial growth factor A‐165 (VEGF‐A165)‐induced cell migration and tube formation in human umbilical vein endothelial cells due to their strong affinity for VEGF‐A165, with a dissociation constant of 2.2 × 10−14 M, over 1600 times stronger than the commercial drug bevacizumab (Avastin). Trypsin digestion coupled with LC‐MS/MS analysis reveals that VEGF‐A165 interacts with SA/DAO‐CNDs through its heparin‐binding domain, leading to activity loss. The SA/DAO‐CNDs demonstrate excellent biocompatibility and potent anti‐angiogenic effects in chicken embryos and rabbit eyes. These findings suggest that SA/DAO‐CNDs hold promise as a therapeutic agent for treating various angiogenesis‐related ocular diseases.
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Research context

NanomaterialsBiomaterials

OpenAlex topics: Nanoplatforms for cancer theranostics · Advanced Nanomaterials in Catalysis · Corneal Surgery and Treatments

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Department of Biomedical Engineering Chang Gung University Taoyuan 33302 Taiwan

Department of Ophthalmology Chang Gung Memorial Hospital Linkou Taoyuan 33305 Taiwan

Department of Materials Engineering Ming Chi University of Technology New Taipei City 24301 Taiwan

Research Center for Chinese Herbal Medicine College of Human Ecology Chang Gung University of Science and Technology Taoyuan 33303 Taiwan

Department of Bioscience and Biotechnology National Taiwan Ocean University Keelung 20224 Taiwan

Center of Excellence for the Oceans National Taiwan Ocean University Keelung 20224 Taiwan

School of Pharmacy College of Pharmacy Kaohsiung Medical University Kaohsiung 80708 Taiwan

Department of Chinese Medicine Chang Gung University Taoyuan 33302 Taiwan

College of Medicine Chang Gung University Taoyuan 33302 Taiwan

Department of Ophthalmology Chang Gung Memorial Hospital Keelung 20401 Taiwan

Department of Biomedical Sciences Chang Gung University Taoyuan 33302 Taiwan

Graduate Institute of Biomedical Sciences Chang Gung University Taoyuan 33302 Taiwan

Center for Advanced Biomaterials and Technology Innovation Chang Gung University Taoyuan 33302 Taiwan

Division of Breast Surgery Department of General Surgery Chang Gung Memorial Hospital Linkou Taoyuan 33305 Taiwan

Funding

Chang Gung Memorial Hospital, Linkou · Chang Gung University · National Science and Technology Council

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