Prolonged release of iloprost enhances pulpal blood flow and dentin bridge formation in a rat model of mechanical tooth pulp exposure
Source abstract
Abstract
The aim of this study was to investigate the effect of prolonged release of iloprost, a prostacyclin analog, on angiogenesis and dental pulp healing in a rat model of mechanical pulp exposure. The profile of iloprost release from poly (lactic-co-glycolic) acid (PLGA) microspheres was evaluated, and expression of vascular endothelial growth factor (VEGF) mRNA was determined. The molars of rats were subjected to mechanical pulp exposure and 5 different forms of treatment: Ca(OH)2, PLGA (blank), iloprost, and iloprost/PLGA. Blood flow was determined at 0, 3, and 7 days using laser Doppler flowmetry. After 30 days, the tooth specimens were collected, and subjected to micro-CT and immunohistological analysis. The results showed that iloprost release from the microspheres was prolonged for 4 days, and that the treatment increased tooth blood flow for up to 7 days. At 30 days, an increase of mineralized tissue formation and dentin bridge formation was observed in the iloprost and iloprost/PLGA microsphere groups. VEGF expression was significantly increased in the iloprost/PLGA microsphere group relative to the other groups. In conclusion, this PLGA microsphere iloprost delivery system significantly increased dental pulp blood flow in a prolonged manner and increased tertiary dentin formation in this rat pulp injury model. Prolonged prostacyclin release could be a potentially useful approach for regeneration of dental pulp.
Source: openalex_inverted_index · Quality: baseline
Research context
OpenAlex topics: Endodontics and Root Canal Treatments · Proteoglycans and glycosaminoglycans research · Advanced Drug Delivery Systems
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Department of Physiology, Faculty of Dentistry, Chulalongkorn University
Excellence Center in Regenerative Dentistry, Faculty of Dentistry, Chulalongkorn University
Graduate School, Oral Biology Program, Faculty of Dentistry, Chulalongkorn University
Faculty of Dentistry, Chulalongkorn University
The Petroleum and Petrochemical College, Chulalongkorn University
Department of Industrial Chemistry, Faculty of Applied Science, King Mongkut University of Technology North
Department of Anatomy, Faculty of Dentistry, Chulalongkorn University
References (38)
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