Use of Bacteria as Fluorescent Penetrant for Penetrant Testing (PT)
Source abstract
Abstract
Penetrant testing (PT) is a Nondestructive testing (NDT) method used for identifying and revealing surface cracks and defects in components. Using bacteria-based suspension as a fluorescent penetrant leads a new approach to PT with the advantages of using eco-friendly chemicals, fewer steps for test process and achieving low cost. The aim of the current study is to synthesize a new fluorescein isothiocyanate (FITC) conjugated Escherichia coli (E. coli) bacteria (FITC-E. coli) as fluorescent penetrant for detection of cracks on test materials. For this purpose, we tested performance of proposed bacteria suspension by means of its detection sensitivity using standardize test panels. Crack centers (6.35, 3.97 and 2.38 mm) and defects (0.2 × 0.4, 0.3 × 0.4 and 0.4 × 0.4 mm) were successfully detected via FITC-E. coli. Experimental results showed that the FITC-E. coli is efficient for detecting the cracks and defects. Research into development of bacteria-based fluorescent penetrant may bring new opportunities for NDT applications in the future.
Source: openaire · Quality: candidate unverified · Source record ↗
Research context
OpenAlex topics: Bacterial biofilms and quorum sensing · Antimicrobial Resistance in Staphylococcus · Biosensors and Analytical Detection
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
References (12)
Show source-provided references
1. Lundh, M.: Automatic crack detection in forged metal parts an image analysis approach. Department of Signals and Systems Division of Signal Processing and Biomedical Engineering, Master of Science Thesis, Chalmers Universıty of Technology (2012)
2. D Devries 2015 Nondestructive flaw detection in metallic components Quality. 54 8 30
3. Migoun, N.P., Delenkovsky, N.V.: The ways of penetrant testing applicability for rough surfaces. In: 17th World Conference on Nondestructive Testing, Shanghai, pp. 25–28
4. D Popescu FD Anania CE Cotet CG Amza 2013 Fully-automated liquid penetrant inspection line simulation model for increasing productivity Int. J. Simul. Model. 12 2 82 93 https://doi.org/10.2507/IJSIMM12(2)2.22510.2507/IJSIMM12(2)2.225 ↗
5. K Daneshvar B Dogan 2010 Application of quantum dots as a fluorescent-penetrant for weld crack detection Mater. High Temp. 27 3 179 182 https://doi.org/10.3184/096034010X1281374466098810.3184/096034010X12813744660988 ↗
6. TG Santos RM Miranda CCCR Carvalho 2014 A new NDT technique based on bacterial cells to detect micro surface defects NDT&E Int. 63 43 49 https://doi.org/10.1016/j.ndteint.2014.01.00610.1016/j.ndteint.2014.01.006 ↗
7. Sezgin, H.S.: Investigation of usability radiolabeled CdSe/ZnS quantum dots with 99mTc as a method of nondestructive test in industry, Master of Science Thesis, Institue of Nuclear Sciences, Ege University Izmir (2012)
8. Siores, E., Lamb, A.S., Swallow, L.M.: Bacterial magnetic particles for applications in NDT&E, detection of flaws and cracks in sample materials. In: Proceeding of the 17th World Conference. Nondestructive. Test, pp. 25–28 (2008)
9. American Type Culture Collection (ATCC) Product sheet: Escherichia coli (ATCC® 15597™) https://www.atcc.org. Accessed 2 Dec 2019
10. DR Absolom FV Lamberti Z Policova W Zingg CJ Oss van AW Neumann 1983 Surface thermodynamics of bacterial adhesion Appl. Environ. Microbiol. 46 1 90 9710.1128/AEM.46.1.90-97.1983 ↗
11. SOF Diniz BM Resende EA Nunan CJR Simal VN Cardoso 1999 99mTechnetium labelled Escherichia coli Appl. Radiat. Isot. 51 1 33 36 https://doi.org/10.1016/S0969-8043(98)00185-710.1016/S0969-8043(98)00185-7 ↗
12. N Nakamura GJ Burgess K Yagiuda S Kudo T Sakaguchi T Matsunaga 1993 Detection and removal of Escherichia coli using fluorescein isothiocyanate conjugated monoclonal antibody immobilized on bacterial magnetic particles Anal. Chem. 65 15 2036 2039 https://doi.org/10.1021/ac00063a01810.1021/ac00063a018 ↗