Bioresorbable poly(lactic acid) and organic quantum dot-based nanocomposites: luminescent scaffolds for enhanced osteogenesis and real-time monitoring
Source abstract
Abstract
Globally, millions require bone replacements with the help of scaffolds to enable bone tissue regeneration. However, scaffold manufacturing techniques do not allow precise control over scaffold geometry and provide diagnostics on scaffold-cell status. Towards addressing these challenges, we studied the use of optimized 3D printing of bioresorbable scaffolds with fluorescent carbon quantum dots (CDs). These can overcome the challenges with toxic and invasive dyes used in clinical settings as probes, while promoting cellular adhesion, proliferation, and osteogenic differentiation. We developed a biodegradable polymer nanocomposite based on PLA with CDs to produce smart biomaterials. As a luminescent, biocompatible material with low toxicity, CDs allowed us to fabricate functionalized biochemical structures that were shown to enhance cell proliferation and promote cell-to-cell communication. The composites were tested in vitro in a cellular microenvironment with optimized 3D-printed scaffolds to demonstrate enhancements in cellular proliferation by 32%, bone mineralization by 8% and osteogenic differentiation by 8% with concomitant negligible cytotoxic effect. Graphic abstract
Source: openalex_inverted_index · Quality: baseline
Research context
OpenAlex topics: Carbon and Quantum Dots Applications · Nanocluster Synthesis and Applications · Graphene and Nanomaterials Applications
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
References (28)
Show source-provided references
1. Gremare, A., Guduric, V., Bareille, R., Heroguez, V., Latour, S., L’Heureux, N., Fricain, J.C., Catros, S., Le Nihouannen, D.: Characterization of printed pla scaffolds for bone tissue engineering. J. Biomed. Mater. Res. A. 106, 887–894 (2018)10.1002/jbm.a.36289 ↗
2. Dave, K., Gomes, V.G.: Interactions at scaffold interfaces: effect of surface chemistry, structural attributes and bioaffinity. Mater. Sci. Eng. 105, 110078 (2019)10.1016/j.msec.2019.110078 ↗
3. Hassan, M., Dave, K., Chandrawati, R., Dehghani, F., Gomes, V.G.: 3d printing of biopolymer nanocomposites for tissue engineering: nanomaterials, processing and structure-function relation. Eur. Polym. J. 121, 109340 (2019)10.1016/j.eurpolymj.2019.109340 ↗
4. Catros, S., Guillemot, F., Nandakumar, A., Ziane, S., Moroni, L., Habibovic, P., van Blitterswijk, C., Rousseau, B., Chassande, O., Amedee, J., Fricain, J.C.: Layer-by-layer tissue microfabrication supports cell proliferation in vitro and in vivo. Tissue Eng. Part C Methods 18, 62–70 (2012)10.1089/ten.tec.2011.0382 ↗
5. Barbieri, D., de Bruijn, J.D., Luo, X., Fare, S., Grijpma, D.W., Yuan, H.: Controlling dynamic mechanical properties and degradation of composites for bone regeneration by means of filler content. J. Mech. Behav. Biomed. Mater. 20, 162–172 (2013)10.1016/j.jmbbm.2013.01.012 ↗
6. Oberhauser, J.P., Hossainy, S., Rapoza, R.J.: Design principles and performance of bioresorbable polymeric vascular scaffolds. EuroIntervention 5(Suppl F), F15–F22 (2009)10.4244/EIJV5IFA3 ↗
7. Onuma, Y., Serruys, P.W.: Bioresorbable scaffold: The advent of a new era in percutaneous coronary and peripheral revascularization? Circulation 123, 779–797 (2011)10.1161/CIRCULATIONAHA.110.971606 ↗
8. Ang, H.Y., Bulluck, H., Wong, P., Venkatraman, S.S., Huang, Y., Foin, N.: Bioresorbable stents: current and upcoming bioresorbable technologies. Int. J. Cardiol. 228, 931–939 (2017)10.1016/j.ijcard.2016.11.258 ↗
9. Vedhanayagam, M., Raja, I.S., Molkenova, A., Atabaev, T.S., Sreeram, K.J., Han, D.W.: Carbon dots-mediated fluorescent scaffolds: Recent trends in image-guided tissue engineering applications. Int. J. Mol. Sci. (2021). https://doi.org/10.3390/ijms2210537810.3390/ijms22105378 ↗
10. Kim, K., Fisher, J.P.: Nanoparticle technology in bone tissue engineering. J. Drug Target. 15, 241–252 (2007)10.1080/10611860701289818 ↗
11. Eivazzadeh-Keihan, R., Maleki, A., de la Guardia, M., Bani, M.S., Chenab, K.K., Pashazadeh-Panahi, P., Baradaran, B., Mokhtarzadeh, A., Hamblin, M.R.: Carbon based nanomaterials for tissue engineering of bone—building new bone on small black scaffolds: a review. J. Adv. Res. 18, 185–201 (2019)10.1016/j.jare.2019.03.011 ↗
12. Hoang, V.C., Dave, K., Gomes, V.G.: Carbon quantum dot-based composites for energy storage and electrocatalysis: mechanism, applications and future prospects. Nano Energy 66, 104093 (2019)10.1016/j.nanoen.2019.104093 ↗
13. Yan, C., Ren, Y., Sun, X., Jin, L., Liu, X., Chen, H., Wang, K., Yu, M., Zhao, Y.: Photoluminescent functionalized carbon quantum dots loaded electroactive silk fibroin/pla nanofibrous bioactive scaffolds for cardiac tissue engineering. J. Photochem. Photobiol. B. 202, 111680 (2020)10.1016/j.jphotobiol.2019.111680 ↗
14. Li, P., Liu, S., Yang, X., Du, S., Tang, W., Cao, W., Zhou, J., Gong, X., Xing, X.: Low-drug resistance carbon quantum dots decorated injectable self-healing hydrogel with potent antibiofilm property and cutaneous wound healing. Chem. Eng. J. 403, 126387 (2021)10.1016/j.cej.2020.126387 ↗
15. Jin, L., Ren, K., Xu, Q., Hong, T., Wu, S., Zhang, Y., Wang, Z.: Multifunctional carbon dots for live cell staining and tissue engineering applications. Polym. Compos. 39, 73–80 (2018)10.1002/pc.23903 ↗
16. Yang, M., Meng, Y., Liu, J., Yu, W., Yang, B.: Facile synthesis of Mg2+-doped carbon dots as novel biomaterial inducing cell osteoblastic differentiation. Part. Part. Syst. Charact. 36, 1800315 (2019)10.1002/ppsc.201800315 ↗
17. Shafiei, S., Omidi, M., Nasehi, F., Golzar, H., Mohammadrezaei, D., Rezai Rad, M., Khojasteh, A.: Egg shell-derived calcium phosphate/carbon dot nanofibrous scaffolds for bone tissue engineering: Fabrication and characterization. Mater. Sci. Eng. 100, 564–575 (2019)10.1016/j.msec.2019.03.003 ↗
18. Windhab N, Mirabet MM, Benedikt A, Engel A, Gomes VG, Hassan M, Nasrin A, Dave K, Mahmud Z (2020) Methods and composition thereof of super hydrophile bioresorbable polymer composites for real time monitoring of scaffolds for tissue engineering. European Provisional Patent Application 20205326.0
19. Nasrin, A., Hassan, M., Gomes, V.G.: Two-photon active nucleus-targeting carbon dots: enhanced ros generation and photodynamic therapy for oral cancer. Nanoscale 12, 20598–20603 (2020)10.1039/D0NR05210H ↗
20. Shin, J.Y., Jeong, S.J., Lee, W.K.: Fabrication of porous scaffold by ternary combination of chitosan, gelatin, and calcium phosphate for tissue engineering. J. Ind. Eng. Chem. 80, 862–869 (2019)10.1016/j.jiec.2019.07.042 ↗
21. Revati, R., Abdul Majid, M.S., Ridzuan, M.J.M., Normahira, M., Mohd Nasir, N.F., Cheng, E.M.: Biodegradation of PLA-pennisetum purpureum based biocomposite scaffold. J. Phys. Conf. Ser. 908(1), 012029 (2017)10.1088/1742-6596/908/1/012029 ↗
22. Hassan, M., Gomes, V.G., Dehghani, A., Ardekani, S.M.: Engineering carbon quantum dots for photomediated theranostics. Nano Res. 11, 1–41 (2017)10.1007/s12274-017-1616-1 ↗
23. Ye, R., Xiang, C., Lin, J., Peng, Z., Huang, K., Yan, Z., Cook, N.P., Samuel, E.L., Hwang, C.C., Ruan, G., Ceriotti, G., Raji, A.R., Marti, A.A., Tour, J.M.: Coal as an abundant source of graphene quantum dots. Nat. Commun. 4, 2943 (2013)10.1038/ncomms3943 ↗
24. Zhu, S., Song, Y., Zhao, X., Shao, J., Zhang, J., Yang, B.: The photoluminescence mechanism in carbon dots (graphene quantum dots, carbon nanodots, and polymer dots): current state and future perspective. Nano Res. 8, 355–381 (2015)10.1007/s12274-014-0644-3 ↗
25. Ahmed, K., Kawakami, M., Khosla, A., Furukawa, H.: Soft, conductive nanocomposites based on ionic liquids/carbon nanotubes for 3D printing of flexible electronic devices. Polym. J. 51, 511–521 (2019)10.1038/s41428-018-0166-z ↗
26. Wu, L., Magaz, A., Wang, T., Liu, C., Darbyshire, A., Loizidou, M., Emberton, M., Birchall, M., Song, W.: Stiffness memory of indirectly 3d-printed elastomer nanohybrid regulates chondrogenesis and osteogenesis of human mesenchymal stem cells. Biomaterials 186, 64–79 (2018)10.1016/j.biomaterials.2018.09.013 ↗
27. Garlotta, D., Doane, W., Shogren, R., Lawton, J., Willett, J.L.: Mechanical and thermal properties of starch-filled poly(d, l-lactic acid)/poly(hydroxy ester ether) biodegradable blends. J. Appl. Polym. Sci. 88, 1775–1786 (2003)10.1002/app.11736 ↗
28. Guloglu, G.E., Altan, M.C.: Moisture absorption of carbon/epoxy nanocomposites. J. Compos. Sci. 4, 21 (2020)10.3390/jcs4010021 ↗