Synthesis of Biopolymeric α-Lactalbumin Protein Nanoparticles and Nanospheres as Green Nanofluids Using in Drug Delivery and Food Technology

New nanofluids carrier containing fine homogeneous whey protein α-lactalbumin spherical nanoparticles and nanospheres have been synthesized by three methods in this research. Biochemical enzymatic catalysis by V8- protease, chemical hydrolysis (sol-gel assisted self assembly method) using cationic templates and metal ions ligand forming, and also by direct acidic titration method with applying various ethylene glycol derivatives containg alcohol solvents. Repeatability and stability of synthetic nanoparticles and nanospheres milk protein structures in clear and crystalline green water-based nanofluids was confirmed by dynamic light scattering (DLS) analysis and TEM images (2-5nm) even after a one year period of preparation. Fabrication of non-toxic nanosolutions-nanofluids including nonionic and cationic surfactants, cosurfactants and suitable solvents, besides proper incubation temperatures (37-50°C), adjusted aging times and doped of divalent ions–bridging (Ca2+, Mn2+ and Zn2+ ) between specific amino acids and carboxyl groups, are so vital factors in kinetically controlled self-assembly phenomenon of the natural protein building blocks to form high resistant and homogeneous nanoparticles and nanospheres in transparent and clear green nanofluids. Such nanofluids can have many applications in food and pharmaceutical industries for example in formulate foods with high nutritional value in oral and digestive (ingestion) routes, and drug delivery. Interesting textures of SEM images have also shown these new nanobiostructures of natural milk protein morphologies.