Anti Wnt-1 Monoclonal Antibody’s Conjugated with Gold Nanoparticles, Induced Apoptosis on MCF-7 Breast Cancer Cell Lines
Source abstract
Abstract
Wnt-β Catenin pathway has an important role in many cancers. Wnt-1 protein from the Wnt protein family, which regulates this pathway, has a special effect on the development of breast cancer. Monoclonal antibodies attaching to metal nanoparticles have an important role in the diagnosis and treatment of cancers.In this study, the anti-Wnt-1monoclonal antibody was conjugated to the gold nanoparticles synthesized by theTurkevichmethod. Conjugation was achieved using the EDC-NHS method. The density of the monoclonal antibodies bonded to gold nanoparticles was measured by Roche Cobas Integra 400 Plus device. MCF-7 and MDA-MB-231 breast cancer cell lines were treated with conjugated nanoparticles for 48 h, followed by performing a double-staining method to detect apoptosis cells.The results showed that inhibition of Wnt-1 protein in extracellular matrix causes apoptosis and gold nanoparticles to have a positive effect on Anti-Wnt-1monoclonal antibodies, leading to an increase in the number of conjugating nanoparticles.
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Research context
OpenAlex topics: Wnt/β-catenin signaling in development and cancer · RNA Interference and Gene Delivery · Cancer Cells and Metastasis
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Hacettepe University
Kirikkale University
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