Redox-active nanoceria depolarize mitochondrial membrane of human colon cancer cells
Source abstract
Abstract
Nanotherapeutics is emerging as a promising option to the various limitations and side effects associated with conventional chemotherapy. The present study investigates the cytotoxic effect of redox-active cerium oxide nanoparticles (nanoceria) on human colorectal adenocarcinoma-derived cell line (HCT 15). Exposure of these cells to nanoceria for 24 h with concentration ranging between 10 and 100 μM resulted in a significant reduction of cell viability in a dose-dependent manner. Further, at a concentration of 10 µM, nanoceria exhibited time-dependent cytotoxic effect when exposed to the cells for 24, 48, and 72 h. Upon treatment of the cells with nanoceria, reactive oxygen species (ROS) and lipid peroxidation which are indicators of oxidative stress and cytotoxicity increased significantly, in a dose-dependent manner. Nanoceria was also found to depolarize the mitochondrial membrane, thereby collapsing the membrane potential and leading to initiation of apoptosis. Scanning electron microscopic study of nanoceria-treated HCT 15 cells showed morphological changes and loss of filopodia and lamellipodia, indicating arrest of metastatic spread. Summarizing, when cultured HCT 15 cells are exposed to nanoceria, a dose-dependent cytotoxic effect mediated by ROS generation is observed.
Source: openaire · Quality: candidate unverified · Source record ↗
Research context
OpenAlex topics: Advanced Nanomaterials in Catalysis · Carbon and Quantum Dots Applications · Advanced biosensing and bioanalysis techniques
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
References (32)
Show source-provided references
1. Alili L, Sack M, Karakoti AS, Teuber S, Puschmann K, Hirst SM, Reilly CM, Zanger K, Stahl W, Das S, Seal S, Brenneisen P (2011) Combined cytotoxic and anti-invasive properties of redox-active nanoparticles in tumor-stroma interactions. Biomaterials 32:2918–292910.1016/j.biomaterials.2010.12.056 ↗
2. Alili L, Sack M, Montfort C, Giri S, Das S, Carroll KS, Zanger K, Seal S, Brenneisen P (2013) Downregulation of tumor growth and invasion by redox-active nanoparticles. Antioxid Redox Signal 19:765–77810.1089/ars.2012.4831 ↗
3. Asati A, Santra S, Kaittanis C, Perez JM (2010) Surface-charge-dependent cell localization and cytotoxicity of cerium oxide nanoparticles. ACS Nano 4:5321–533110.1021/nn100816s ↗
4. Barchi JJJ, Rittenhouse-Olson K, Svarovsky S (2007) Carbohydrate antigen-nanoparticle conjugates and uses there of as antimetastatic agents in treating cancer. US20070275007 A1
5. Bednarski MD, Guccione S, Li K (2009) Use of targeted cross-linked nanoparticles for in vivo gene delivery. US7514098 B2
6. Boulikas T (2003) Therapy for human cancers using cisplatin and other drugs or genes encapsulated into liposomes. US6511676 B1
7. Byrne JD, Betancourt T, Brannon-Peppas L (2008) Active targeting schemes for nanoparticle systems in cancer therapeutics. Adv Drug Deliv Rev 60:1615–162610.1016/j.addr.2008.08.005 ↗
8. Chidambaram M, Manavalan R, Kathiresan K (2011) Nanotherapeutics to overcome conventional cancer chemotherapy limitations. J Pharm Pharm Sci 14:67–7710.18433/J30C7D ↗
9. Cho K, Wang X, Nie S, Chen ZG, Shin DM (2008) Therapeutic nanoparticles for drug delivery in cancer. Clin Cancer Res 14:1310–131610.1158/1078-0432.CCR-07-1441 ↗
10. Das M, Patil S, Bhargava N, Kang JF, Riedel LM, Seal S, Hickman JJ (2007) Auto-catalytic ceria nanoparticles offer neuroprotection to adult rat spinal cord neurons. Biomaterials 28:1918–192510.1016/j.biomaterials.2006.11.036 ↗
11. Das S, Dowding JM, Klump KE, McGinnis JF, Self W, Seal S (2013) Cerium oxide nanoparticles: applications and prospects in nanomedicine. Nanomedicine (Lond) 8:1483–150810.2217/nnm.13.133 ↗
12. Dowding JM, Seal S, Self WT (2013) Cerium oxide nanoparticles accelerate the decay of peroxynitrite (ONOO). Drug Deliv Transl Res 3:375–37910.1007/s13346-013-0136-0 ↗
13. Gelperina S, Kreuter J, Sabel BA, Schroeder U (2000) Use of drug-loaded nanoparticles for the treatment of cancers. WO2000074658 A1
14. Giri S, Karakoti A, Graham RP, Maguire JL, Reilly CM, Seal S, Rattan R, Shridhar V (2013) Nanoceria: a rare-earth nanoparticle as a novel anti-angiogenic therapeutic agent in ovarian cancer. PLoS ONE 8:e5457810.1371/journal.pone.0054578 ↗
15. Hanley C, Layne J, Punnoose A, Reddy KM, Coombs I, Coombs A, Feris K, Wingett D (2008) Preferential killing of cancer cells and activated human T cells using ZnO nanoparticles. Nanotechnology 19:29510310.1088/0957-4484/19/29/295103 ↗
16. Hassan EM (2002) Elemental nanoparticles of substantially water insoluble materials. US20020119916 A1
17. Heckert EG, Karakoti AS, Seal S, Self WT (2008a) The role of cerium redox state in the SOD mimetic activity of nanoceria. Biomaterials 29:2705–270910.1016/j.biomaterials.2008.03.014 ↗
18. Heckert EG, Seal S, Self WT (2008b) Fenton-like reaction catalyzed by the rare earth inner transition metal cerium. Environ Sci Technol 42:5014–501910.1021/es8001508 ↗
19. Kukowska-Latallo JF, Candido KA, Cao Z, Nigavekar SS, Majoros IJ, Thomas TP, Balogh LP, Khan MK, Baker JR Jr (2005) Nanoparticle targeting of anticancer drug improves therapeutic response in animal model of human epithelial cancer. Cancer Res 65:5317–532410.1158/0008-5472.CAN-04-3921 ↗
20. Kuroda S, Katsuyuki T, Kondo A., Ueda M, Senoo S, Iwabuki H. (2003) Therapeutic medicine for hepatic disease using protein hollow nanoparticle. JP2003286199
21. Kuroda S, Tanizawa K, Kondo A, Ueda M, Seno M, Okajima T (2004) Therapeutic drug using antibody-presenting hollow protein nanoparticles and hollow protein nanoparticles. EP1491210 A1
22. Lin W, Huang YW, Zhou XD, Ma Y (2006) Toxicity of cerium oxide nanoparticles in human lung cancer cells. Int J Toxicol 25(6):451–45710.1080/10915810600959543 ↗
23. Meng L, Yang L, Zhao X, Zhang L, Zhu H, Liu C, Tan W (2012) Targeted delivery of chemotherapy agents using a liver cancer-specific aptamer. PLoS ONE 7:e3343410.1371/journal.pone.0033434 ↗
24. Michael B, Regis C, Annekathrin H, Helmuth M, Marc S, Gleb S, Sergei Z, Heidi Z (2003) Preparation and use of dna-polyelectrolyte nanoparticles for gene transfer. WO2003087384 A1
25. Petit PX, Lecoeur H, Zorn E, Dauguet C, Mignotte B, Gougeon ML (1995) Alterations in mitochondrial structure and function are early events of dexamethasone-induced thymocyte apoptosis. J Cell Biol 130:157–16710.1083/jcb.130.1.157 ↗
26. Pirmohamed T, Dowding JM, Singh S, Wasserman B, Heckert E, Karakoti AS, King JE, Seal S, Self WT (2010) Nanoceria exhibit redox state-dependent catalase mimetic activity. Chem Commun (Camb) 46:2736–273810.1039/b922024k ↗
27. Praetorius NP, Mandal TK (2007) Engineered nanoparticles in cancer therapy. Recent Pat Drug Deliv Formula 1:37–5110.2174/187221107779814104 ↗
28. Thevenot P, Cho J, Wavhal D, Timmons RB, Tang L (2008) Surface chemistry influences cancer killing effect of TiO2 nanoparticles. Nanomedicine 4:226–23610.1016/j.nano.2008.04.001 ↗
29. Venkatesan P, Das S, Krishnan MMR, Chakraborty C, Chaudhury K, Mandal M (2010) Effect of AEE788 and/or Celecoxib on colon cancer cell morphology using advanced microscopic techniques. Micron 41:247–25610.1016/j.micron.2009.10.008 ↗
30. Wang H, Joseph JA (1999) Quantifying cellular oxidative stress by dichlorofluorescein assay using microplate reader. Free Radic Biol Med 27:612–61610.1016/S0891-5849(99)00107-0 ↗
31. Wason MS, Colon J, Das S, Seal S, Turkson J, Zhao J, Baker CH (2012) Sensitization of pancreatic cancer cells to radiation by cerium oxide nanoparticle-induced ROS production. Nanomedicine 9:558–56910.1016/j.nano.2012.10.010 ↗
32. Xue Y, Luan QF, Yang D, Yao X, Zhou KB (2011) Direct evidence for hydroxyl radical scavenging activity of cerium oxide nanoparticles. J Phys Chem C 115:4433–443810.1021/jp109819u ↗