Fe3O4 nanoparticles engineered for plasmid DNA delivery to Escherichia coli
Source abstract
Abstract
Heat shock treatment is the most popular method for transformation of Escherichia coli. We have used 19-nm Fe3O4 nanoparticles for improving heat shock protocol. PGEM-T (3,000 bp) and pCAMBIA (8,428 bp) were used as test plasmids for transformation of competent E. coli cells (strains DH5α and Jm107) obtained from heat shock- and CaCl2-treated bacteria. A combination of heat shock and Fe3O4 nanoparticles led to a significant increase (6–10 fold) in number of transformed colonies in comparison with heat shock alone. The percent increase in transformation efficiency was higher for larger pCAMBIA plasmids compared to PGEM-T. The transformation efficiency decreased in the absence of CaCl2 and increased by addition of glycerol to the bacterial culture.
Source: openaire · Quality: candidate unverified · Source record ↗
Research context
OpenAlex topics: RNA Interference and Gene Delivery · Bacteriophages and microbial interactions · Bacterial Genetics and Biotechnology
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
References (40)
Show source-provided references
1. Chen J-Y, Liao Y-L, Wang T-H, Lee W-C (2006) Transformation of Escherichia coli mediated by magnetic nanoparticles in pulsed magnetic field. Enzyme Microbial Technol 39:366–37010.1016/j.enzmictec.2005.11.035 ↗
2. Cohen SN, Chang AC, Hsu L (1972) Nonchromosomal antibiotic resistance in bacteria: genetic transformation of Escherichia coli by R-factor DNA. Proc Natl Acad Sci USA 69:2110–211410.1073/pnas.69.8.2110 ↗
3. Cymbalyuk E, Chernomordik L, Broude N, Chizmadzhev YA (1988) Electro-stimulated transformation of E. coli cells pretreated by EDTA solution. FEBS Lett 234:203–20710.1016/0014-5793(88)81334-6 ↗
4. Divya Prakash G, Anish R, Jagadeesh G, Chakravortty D (2011) Bacterial transformation using micro-shock waves. Anal Biochem 419:292–30110.1016/j.ab.2011.08.038 ↗
5. Du G, Yang G, Qu Y, Chen J, Lun S (2005) Effects of glycerol on the production of poly (γ-glutamic acid) by Bacillus licheniformis. Process Biochem 40:2143–214710.1016/j.procbio.2004.08.005 ↗
6. Fan JS, Palade P (1998) Perforated patch recording with β-escin. Pflügers Arch 436:1021–102310.1007/PL00008086 ↗
7. Fregel R, Rodriguez V, Cabrera V (2008) Microwave improved Escherichia coli transformation. Lett Appl Microbiol 46:498–49910.1111/j.1472-765X.2008.02333.x ↗
8. Hanahan D (1983) Studies on transformation of Escherichia coli with plasmids. J Mol Biol 166:557–58010.1016/S0022-2836(83)80284-8 ↗
9. Heidari Majd M, Asgari D, Barar J, Valizadeh H, Kafil V, Abadpour A, Moumivand E, Mojarrad JS, Rashidi MR, Coukos G, Omidi Y (2013a) Tamoxifen loaded folic acid armed PEGylated magnetic nanoparticles for targeted imaging and therapy of cancer. Colloids Surf B Biointerfaces 106:117–12510.1016/j.colsurfb.2013.01.051 ↗
10. Heidari Majd M, Asgari D, Barar J, Valizadeh H, Kafil V, Coukos G, Omidi Y (2013b) Specific targeting of cancer cells by multifunctional mitoxantrone-conjugated magnetic nanoparticles. J Drug Target 21:328–34010.3109/1061186X.2012.750325 ↗
11. Inoue H, Nojima H, Okayama H (1990) High efficiency transformation of Escherichia coli with plasmids. Gene 96:23–2810.1016/0378-1119(90)90336-P ↗
12. Kang SM, Choi IS, Lee K-B, Kim Y (2009) Bioconjugation of poly (poly (ethylene glycol) methacrylate)-coated iron oxide magnetic nanoparticles for magnetic capture of target proteins. Macromol Res 17:259–26410.1007/BF03218689 ↗
13. Kobayashi T, Akinori O, Shibuya I (1986) Membrane phospholipid synthesis in Escherichia coli: alteration by glycerol and physiological consequences in a pss mutant. J Biochem 99:1393–140010.1093/oxfordjournals.jbchem.a135608 ↗
14. Konishi M, Watanabe M (1995) Molecular size-dependent leakage of intracellular molecules from frog skeletal muscle fibers permeabilized with β-escin. Pflügers Archiv 429:598–60010.1007/BF00704168 ↗
15. Kurien BT, Scofield RH (1995) Polyethylene glycol-mediated bacterial colony transformation. Biotechniques 18:1023–1026
16. Kusters R, Breukink E, Gallusser A, Kuhn A, de Kruijff B (1994) A dual role for phosphatidylglycerol in protein translocation across the Escherichia coli inner membrane. J Biol Chem 269:1560–156310.1016/S0021-9258(17)42293-9 ↗
17. Laurent S, Dutz S, Häfeli UO, Mahmoudi M (2011) Magnetic fluid hyperthermia: focus on superparamagnetic iron oxide nanoparticles. Adv Colloid Interface Sci 166:8–2310.1016/j.cis.2011.04.003 ↗
18. Laurent S, Saei AA, Behzadi S, Panahifar A, Mahmoudi M (2014) Superparamagnetic iron oxide nanoparticles for delivery of therapeutic agents: opportunities and challenges. Expert Opin Drug Deliv [Epub ahead of print]10.1517/17425247.2014.924501 ↗
19. Lill R, Dowhan W, Wickner W (1990) The ATPase activity of SecA is regulated by acidic phospholipids, SecY, and the leader and mature domains of precursor proteins. Cell 60:271–28010.1016/0092-8674(90)90742-W ↗
20. Lu CW, Hung Y, Yao M, Chung T, Lin Y (2007) Bifunctional magnetic silica nanoparticles for highly efficient human stem cell labeling. Nano Lett 7:149–15410.1021/nl0624263 ↗
21. Mah C, Fraites TJ Jr, Zolotukhin I, Song S, Flotte TR, Dobson J, Batich C, Byrne BJ (2002) Improved method of recombinant AAV2 delivery for systemic targeted gene therapy. Mol Ther 6:106–11210.1006/mthe.2001.0636 ↗
22. Mandel M, Higa A (1970) Calcium-dependent bacteriophage DNA infection. J Mol Biol 53:159–16210.1016/0022-2836(70)90051-3 ↗
23. McBain S, Yiu H, El Haj A, Dobson J (2007) Polyethyleneimine functionalized iron oxide nanoparticles as agents for DNA delivery and transfection. J Mater Chem 17:2561–256510.1039/b617402g ↗
24. Mirshafiee V, Mahmoudi M, Lou K, Cheng J, Kraft ML (2013) Protein corona significantly reduces active targeting yield. Chem Commun 49:2557–255910.1039/c3cc37307j ↗
25. Opekarova M, Tanner W (2003) Specific lipid requirements of membrane proteins—a putative bottleneck in heterologous expression. Biochim Biophys Acta 1610:11–2210.1016/S0005-2736(02)00708-3 ↗
26. Park HY, Park HY, Schadt MJ, Lingyan I-Im, Lim S, Njoki PN, Kim SK, Jang MY, Luo J, Zhong CJ (2007) Fabrication of magnetic core@shell Fe oxide@Au nanoparticles for interfacial bioactivity and bio-separation. Langmuir 23:9050–905610.1021/la701305f ↗
27. Ravnikar M, Irman A, Radic N, Lunder M, Strukelj B (2009) Chemoporation using saponins or cholates: an alternative method for transformation of bacterial cells. Biotechnol Lett 31:1943–194610.1007/s10529-009-0101-7 ↗
28. Rojas-Chapana J, Troszczynska J, Firkowska I, Morsczeck C, Giersig M (2005) Multi-walled carbon nanotubes for plasmid delivery into Escherichia coli cells. Lab Chip 5:536–53910.1039/b500681c ↗
29. Saei AA, Mahmoudi M (2014). Toxicity of nanoparticles. in Nanoparticles for delivery of biotherapeutics, Future Med. [Epub ahead of print]
30. Saei AA, Najafi-Marandi P, Abhari A, de la Guardia M, Dolatabadi JEN (2013) Electrochemical biosensors for glucose based on metal nanoparticles. Trends Anal Chem 42:216–22710.1016/j.trac.2012.09.011 ↗
31. Sambrook J, Russell DW (2006) The Hanahan method for preparation and transformation of competent E. coli: high-efficiency transformation. Cold Spring Harbor Protocols 2006:pdb. prot3942
32. Shanehbandi D, Saei AA, Zarredar H, Barzegari A (2013) Vibration and glycerol-mediated plasmid DNA transformation for Escherichia coli. FEMS Microbiol Lett 348:74–7810.1111/1574-6968.12247 ↗
33. Sheng Y, Mancino V, Birren B (1995) Transformation of Escherichia coli with large DNA molecules by electroporation. Nucleic Acids Res 23:1990–199610.1093/nar/23.11.1990 ↗
34. Singh M, Yadav A, Ma X, Amoah E (2010) Plasmid DNA transformation in Escherichia Coli: effect of heat shock temperature, duration, and cold incubation of CaCl2 treated cells. Intl J Biotechnol Biochem 6:561–568
35. Song Y et al (2007) Ultrasound-mediated DNA transfer for bacteria. Nucleic Acids Res 35:e12910.1093/nar/gkm710 ↗
36. Stuy JH, Walter RB (1986) Effect of glycerol on plasmid transfer in genetically competent Haemophilus influenzae. Mol Gen Genet 203:296–29910.1007/BF00333969 ↗
37. Tang X et al (1994) The optimization of preparations of competent cells for transformation of E. coli. Nucleic Acids Res 22:2857–285810.1093/nar/22.14.2857 ↗
38. Wang B, Xu C, Xie J, Yang Z, Sun S (2008) pH controlled release of chromone from chromone-Fe3O4 nanoparticles. J Am Chem Soc 130:14436–1443710.1021/ja806519m ↗
39. Widder KJ, Senyei AE, Scarpelli DG (1978) Magnetic microspheres: a model system for site specific drug delivery in vivo. Proc Soc Exp Biol Med 158:141–14610.3181/00379727-158-40158 ↗
40. Xu Z, Shen C, Hou Y, Gao H, Sun S (2009) Oleylamine as both reducing agent and stabilizer in a facile synthesis of magnetite nanoparticles. Chem Mater 21:1778–178010.1021/cm802978z ↗