Chip‐Based Cell Electrofusion

Abstract Cell fusion is a rapid developing cytobiological method utilizing engineering approaches to fuse two or more cells into one hybrid. The hybrid cell can be further cultivated into a new species or a cell‐engineering product. After many years of development, cell fusion is becoming a powerful tool for biological, medical and agricultural research. At present, electrofusion is widely used to achieve cell fusion due to its high efficiency and maneuverability. On‐chip cell electrofusion is receiving more and more attention as a result of its superior performance. This review will discuss the basic theory of chip‐based cell electrofusion, the pairing method, low‐ and high‐throughput cell electrofusion, and the possible ways to overcome the major limitations in existing electrofusion methods.