Novel gamma irradiated agarose-gelatin-hydroxyapatite nanocomposite scaffolds for skin tissue regeneration
Source abstract
Abstract
Abstract Agarose-gelatin-hydroxyapatite composites prepared by freeze-drying technique were gamma irradiated with various doses (25 kGy, 50 kGy and 100 kGy). X-ray Diffraction (XRD) analysis revealed the pure phase of HAp and the intensity of prominent planes of hydroxyapatite (Ca 10 (PO 4 ) 6 (OH) 2 , HAp) were found to decrease on irradiation. Fourier Transform Infrared spectra (FTIR) showed functional groups of HAp and polymer composites, and higher disorder of the polymer matrix on irradiation. In addition, gamma irradiation led to a drastic reduction in the wettability (62%) and the compressive modulus (76%) of the scaffolds. There was significant enhancement (113%) in pore size of the scaffolds at higher fluence (100 kGy). The swelling and the dissolution studies of the gamma irradiated scaffolds showed that it had an appreciable change in the scaffold's mechanical and biological properties viz., compressive modulus, cell proliferation, hemolysis etc. The irradiated biomaterials exhibited enhanced hemocompatibility, antimicrobial activity and cell viability. The above results clearly reveal that the gamma irradiation is a suitable tool to tailor the multifunctional properties of the composites and could be used for various biomedical applications.
Source: openaire · Quality: candidate unverified · Source record ↗
Research context
OpenAlex topics: Bone Tissue Engineering Materials · Electrospun Nanofibers in Biomedical Applications · Additive Manufacturing and 3D Printing Technologies
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
References (44)
Show source-provided references
1. An alternative technique to shape scaffolds with hierarchial porosity at physiological temperature10.1016/j.actbio.2009.10.049 ↗
2. Super macroporous chitosan agarose-gelatin cryogels: in vitro characterization and in vivo assessment for cartilage tissue engineering10.1098/rsif.2010.0455 ↗
3. Structure and properties of nano-hydroxyapatite/polymer composite scaffolds for bone tissue engineering10.1016/j.biomaterials.2003.12.005 ↗
4. Polymeric scaffolds for bone tissue engineering10.1023/B:ABME.0000017544.36001.8e ↗
5. Biodegradable polymer matrix nanocomposites for tissue engineering: a review10.1016/j.polymdegradstab.2010.06.007 ↗
6. Biodegradable polymer scaffolds with well-defined interconnected spherical pore network10.1089/107632701300003269 ↗
7. Hydroxyapatite gelatin films: a structural and mechanical characterization10.1016/S0142-9612(97)00194-4 ↗
8. Development of mammalian cell-enclosing sub sieve size agarose capsules (<100µm) for cell therapy10.1016/j.biomaterials.2004.11.043 ↗
9. Synthesis of an agarose gelatin conjugate for use as a tissue engineering scaffold10.1263/jbb.103.22 ↗
10. Biodisposition characteristics of N-succinyl–chitosan and glycol–chitosan in normal and tumor bearing mice10.1248/bpb.22.179 ↗
11. Growth and differentiation of mouse osteoblasts on chitosan–collagen sponges10.1016/j.ijom.2006.09.023 ↗
12. Formation of monolithic silica gel microhoneycombs (SMHs) using pseudosteady state growth of microstructural ice crystals10.1039/b316597c ↗
13. Making tissue engineering scaffolds work. Review on the application of solid freeform fabrication technology to the production of tissue engineering scaffolds10.22203/eCM.v005a03 ↗
14. Fabrication of porous gelatin scaffolds for tissue engineering10.1016/S0142-9612(99)00036-8 ↗
15. Influence of irradiation sterilization on polymers used as drug carriers — a review10.3109/03639049709148693 ↗
16. The effect of gamma radiation on molecular stability and mechanical properties of biodegradable polyurethanes for medical applications10.1016/S0141-3910(02)00362-2 ↗
17. Review of the effects of gamma radiation on pharmaceutical materials10.1177/088532829501000104 ↗
18. Tetracycline-HCl-loaded poly(DL-lactide-co-glycolide) microspheres prepared by a spray drying technique: influence of γ-irradiation on radical formation and polymer degradation10.1016/S0168-3659(98)00170-9 ↗
19. Gamma irradiation effects on poly(dl-lactictide-co-glycolide) microspheres10.1016/S0168-3659(98)00082-0 ↗
20. Preparation and characterization of polyvinyl alcohol-gelatin hydrogel membranes for biomedical applications10.1208/pt080121 ↗
21. New x-ray diffraction results from agarose: extended single helix structure and implications for gelation mechanism10.1002/bip.360280802 ↗
22. Physicochemical characterization of the superhydrophilic, magnesium and silver ions co-incorporated nanocrystalline hydroxyapatite, synthesized by microwave processing10.1016/j.ceramint.2014.05.088 ↗
23. Study of optical band gap, carbonaceous clusters and structuring in CR-39 and PET polymers irradiated by 100MeV O7+ ions10.1016/j.physb.2008.09.033 ↗
24. Effect of swift heavy ion irradiation on structure, optical, and gas sensing properties of SnO2 thin films10.1016/j.nimb.2008.02.062 ↗
25. Grain growth and crack formation in NiO thin films by swift heavy ion irradiation10.1016/j.nimb.2009.11.004 ↗
26. Characterization and cytocompatibility of hybrid aminosilane-agarose hydrogel scaffolds10.1116/1.3388182 ↗
27. Evaluation of cross-linking methods for electrospun gelatin on cell growth and viability10.1021/bm900036s ↗
28. Blend fibers of chitosan–agarose by electrospinning10.1016/j.matlet.2009.08.051 ↗
29. Chitosan–gelatin scaffolds for tissue engineering: physico-chemical properties and biological response of buffalo embryonic stem cells and transfectant of GFP–buffalo embryonic stem cells10.1016/j.actbio.2009.05.012 ↗
30. Fabrication of porous hydroxyapatite-gelatin composite scaffolds for bone tissue engineering
31. In situ hydroxyapatite synthesis: influence of collagen on its structural and morphological characteristic
32. Effect of 1.25MeV gamma irradiation in α-phased PVDF10.1016/j.nimb.2009.08.026 ↗
33. Effect of solvent; enhancing the wettability and engineering the porous structure of calcium phosphate/agarose composite for drug delivery10.1039/C4RA14584D ↗
34. Designing Superoleophobic Surfaces10.1126/science.1148326 ↗
35. Surface free energy of ultra-high molecular weight polyethylene modified by electron and gamma irradiation10.1016/j.apsusc.2009.04.176 ↗
36. Surface modification of nanocrystalline calcium phosphate bioceramic by low energy nitrogen ion implantation10.1016/j.ceramint.2012.09.081 ↗
37. Tunable hydroxyapatite wettability: effect on adhesion of biological molecules10.1016/j.procbio.2006.06.006 ↗
38. Bacterial adhesion to glass and metal-oxide surfaces10.1016/j.colsurfb.2004.05.006 ↗
39. Room temperature synthesis of agarose/sol–gel glass pieces with tailored interconnected porosity10.1002/jbm.a.30724 ↗
40. Preparation and characterization of novel chitosan/gelatin membranes using chitosan hydrogel10.1016/j.carbpol.2008.10.015 ↗
41. Wear characteristic and bi ocompatibility of some polymer composite acetabular cups10.1016/S0043-1648(03)00535-0 ↗
42. Biologically active polymers. V. Synthesis and antimicrobial activity of modified poly(glycidylmethacrylate-co-2-hydroxyethyl methacrylate) derivatives with quaternary ammonium and phosphonium salts10.1002/pola.10325 ↗
43. Polymeric materials with antimicrobial activity10.1016/j.progpolymsci.2011.08.005 ↗
44. Cell proliferation and migration in silk fibroin 3D scaffolds10.1016/j.biomaterials.2009.02.006 ↗