Tunable plasma coating method modulates surface charge and protein corona of cell-derived nanovesicles for improved systemic drug delivery
Source abstract
Abstract
Summary Cell-derived nanovesicles are emerging as versatile carriers for therapeutic delivery, but their surface charge and protein corona critically influence their biological behavior. Here, plasma coating is introduced as a rapid, tunable, and reagent-free strategy to transiently modify vesicle surface properties. Plasma exposure reverses the membrane charge of cell membrane-derived nanovesicles in a treatment time-dependent and reversible manner. Moreover, plasma treatment modulates the density and composition of the protein corona, yielding a profile associated with reduced immune recognition and enhanced cellular interaction. These changes collectively improve cellular uptake, drug delivery efficiency, and therapeutic activity in vivo. This controllable and biocompatible surface-engineering approach offers a scalable platform for optimizing nanovesicle-based drug delivery and related biomedical applications.
Source: openalex_inverted_index · Quality: candidate unverified · Source record ↗
Research context
OpenAlex topics: Extracellular vesicles in disease · Nanoplatforms for cancer theranostics · RNA Interference and Gene Delivery
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
References (76)
Show source-provided references
1. Smart cancer nanomedicine10.1038/s41565-019-0567-y ↗
2. Delivering the power of nanomedicine to patients today10.1016/j.jconrel.2020.07.007 ↗
3. Lipid-based nanovesicles for nanomedicine10.1039/C6CS00409A ↗
4. Biological functions and current advances in isolation and detection strategies for exosome nanovesicles10.1002/smll.201702153 ↗
5. Impact of nanotechnology on drug delivery10.1021/nn900002m ↗
6. Nanovesicles based drug targeting to control tumor growth and metastasis10.1016/j.adcanc.2022.100083 ↗
7. Overcome the barriers of the skin: exosome therapy10.1186/s40824-021-00224-8 ↗
8. Immunogenic hybrid nanovesicles of liposomes and tumor-derived nanovesicles for cancer immunochemotherapy10.1021/acsnano.0c09681 ↗
9. Neutrophil Nanovesicle Protects against Experimental Autoimmune Encephalomyelitis through Enhancing Myelin Clearance by Microglia10.1021/acsnano.2c07798 ↗
10. Extruded Mesenchymal stem cell Nanovesicles are equally potent to natural extracellular vesicles in cardiac repair10.1021/acsami.1c08044 ↗
11. Porous microneedle patch with sustained delivery of extracellular vesicles mitigates severe spinal cord injury10.1038/s41467-023-39745-2 ↗
12. Exosome-coated oxygen nanobubble-laden hydrogel augments intracellular delivery of exosomes for enhanced wound healing10.1038/s41467-024-47696-5 ↗
13. In vivo differentiation of therapeutic insulin-producing cells from bone marrow cells via extracellular vesicle-mimetic nanovesicles10.1021/acsnano.5b02997 ↗
14. Bioinspired nanovesicles convert the skeletal endothelium-associated secretory phenotype to treat osteoporosis10.1021/acsnano.2c03781 ↗
15. The role of surface charge in cellular uptake and cytotoxicity of medical nanoparticles10.2147/IJN.S36111 ↗
16. Effect of surface properties on nanoparticle–cell interactions10.1002/smll.200901158 ↗
17. Effects of particle size and surface modification on cellular uptake and biodistribution of polymeric nanoparticles for drug delivery10.1007/s11095-012-0958-3 ↗
18. Effect of nanoparticle surface charge at the plasma membrane and beyond10.1021/nl101140t ↗
19. Preferential cancer cell self-recognition and tumor self-targeting by coating nanoparticles with homotypic cancer cell membranes10.1021/acs.nanolett.6b02786 ↗
20. The effect of surface charge on in vivo biodistribution of PEG-oligocholic acid based micellar nanoparticles10.1016/j.biomaterials.2011.01.021 ↗
21. Interaction of positively charged gold nanoparticles with cancer cells monitored by an in situ label-free optical biosensor and transmission electron microscopy10.1021/acsami.8b01546 ↗
22. Polymer-coated nanoparticles interacting with proteins and cells: focusing on the sign of the net charge10.1021/nn3059295 ↗
23. Nanoparticle adhesion to the cell membrane and its effect on nanoparticle uptake efficiency10.1021/ja309812z ↗
24. The crown and the scepter: roles of the protein corona in nanomedicine10.1002/adma.201805740 ↗
25. Nanoparticle protein corona evolution: from biological impact to biomarker discovery10.1039/D1NR06580G ↗
26. Time evolution of the nanoparticle protein corona10.1021/nn901372t ↗
27. The protein corona and its effects on nanoparticle-based drug delivery systems10.1016/j.actbio.2021.05.019 ↗
28. Rapid, deep and precise profiling of the plasma proteome with multi-nanoparticle protein corona10.1038/s41467-020-17033-7 ↗
29. Nanoparticle size and surface properties determine the protein corona with possible implications for biological impacts10.1073/pnas.0805135105 ↗
30. Cold atmospheric plasma delivery for biomedical applications10.1016/j.mattod.2022.03.001 ↗
31. Plasma generation and plasma sources10.1088/0963-0252/9/4/301 ↗
32. Production of antibacterial coatings through atmospheric pressure plasma: a promising alternative for combatting biofilms in the food industry10.1007/s11947-019-02293-z ↗
33. Application of cold plasma technology in the food industry and its combination with other emerging technologies10.1016/j.tifs.2021.06.004 ↗
34. Plasma deposition of optical films and coatings: A review10.1116/1.1314395 ↗
35. Pulsed plasma etching for semiconductor manufacturing10.1088/0022-3727/47/30/303001 ↗
36. Richardson, J.F. (2002). Coulson and Richardson’s Chemical Engineering
37. RNAi delivery by exosome-mimetic nanovesicles–Implications for targeting c-Myc in cancer10.1016/j.biomaterials.2016.06.024 ↗
38. Exosome mimetics: a novel class of drug delivery systems
39. Large-scale generation of cell-derived nanovesicles10.1039/C4NR02391A ↗
40. Bioinspired exosome-mimetic nanovesicles for targeted delivery of chemotherapeutics to malignant tumors10.1021/nn402232g ↗
41. Exosome-mimetic nanoplatforms for targeted cancer drug delivery10.1186/s12951-019-0517-8 ↗
42. A peptide-network weaved nanoplatform with tumor microenvironment responsiveness and deep tissue penetration capability for cancer therapy10.1002/adma.201501502 ↗
43. A cancer cell membrane coated, doxorubicin and microRNA co-encapsulated nanoplatform for colorectal cancer theranostics10.1016/j.omto.2022.12.002 ↗
44. Microfluidic fabrication of cell-derived nanovesicles as endogenous RNA carriers10.1039/C3LC50993A ↗
45. Cell-derived biomimetic nanocarriers for targeted cancer therapy: cell membranes and extracellular vesicles10.1080/10717544.2021.1938757 ↗
46. Doxorubicin-loaded cell-derived nanovesicles: An alternative targeted approach for anti-tumor therapy10.2147/IJN.S131786 ↗
47. Cell membrane-derived nanovesicles as extracellular vesicle-mimetics in wound healing
48. Re-engineering extracellular vesicles as smart nanoscale therapeutics10.1021/acsnano.6b07607 ↗
49. Surface modification of lipid-based nanocarriers: a potential approach to enhance targeted drug delivery10.1021/acsomega.2c05976 ↗
50. Plasma-Engineered PDRN: Surface Charge Neutralization and Nanosizing Enhance Uptake and Regeneration Potential10.3390/pharmaceutics17091136 ↗
51. Tuning hydrated nanoceria surfaces: experimental/theoretical investigations of ion exchange and implications in organic and inorganic interactions10.1021/la904285g ↗
52. Nanoparticle halos: A new colloid stabilization mechanism10.1073/pnas.151063098 ↗
53. Effect of surface charge on the colloidal stability and in vitro uptake of carboxymethyl dextran-coated iron oxide nanoparticles10.1007/s11051-013-1874-0 ↗
54. Extracellular vesicles as tools and targets in therapy for diseases10.1038/s41392-024-01735-1 ↗
55. Study of Interaction Energy between Nanoparticles and Cell Membrane
56. Endocytosis: the nanoparticle and submicron nanocompounds gateway into the cell10.3390/pharmaceutics12040371 ↗
57. Spacer length and serum protein adsorption affect active targeting of trastuzumab-modified nanoparticles
58. Opsonins and dysopsonins of nanoparticles: facts, concepts, and methodological guidelines10.3389/fimmu.2020.567365 ↗
59. Protein corona of nanoparticles: distinct proteins regulate the cellular uptake10.1021/acs.biomac.5b00108 ↗
60. The Differences of Myelosuppression before and after Doxorubicin Chemotherapy in Breast Cancer Patients in Rsup. H. Adam Malik Medan10.52403/ijrr.20210704 ↗
61. Taurine protects doxorubicin-induced hepatotoxicity via its membrane-stabilizing effect in rats10.3390/life13102031 ↗
62. Comprehensive toxicity and immunogenicity studies reveal minimal effects in mice following sustained dosing of extracellular vesicles derived from HEK293T cells10.1080/20013078.2017.1324730 ↗
63. Mesenchymal stem cell-derived extracellular vesicles improve survival and enhance hematopoietic recovery in mice exposed to high-dose irradiation10.1089/scd.2025.0036 ↗
64. Biodistribution of extracellular vesicles following administration into animals: A systematic review10.1002/jev2.12085 ↗
65. A review of NIR dyes in cancer targeting and imaging10.1016/j.biomaterials.2011.06.024 ↗
66. Cyanines during the 1990s: a review10.1021/cr990402t ↗
67. Age-associated interplay between zinc deficiency and Golgi stress hinders microtubule-dependent cellular signaling and epigenetic control10.1016/j.devcel.2024.12.024 ↗
68. Loss of the dermis zinc transporter ZIP13 promotes the mildness of fibrosarcoma by inhibiting autophagy10.1038/s41598-019-51438-9 ↗
69. Extracellular vesicle-mimetic nanovesicles transport LncRNA-H19 as competing endogenous RNA for the treatment of diabetic wounds10.1080/10717544.2018.1425774 ↗
70. Facile and scalable fabrication of exosome-mimicking nanovesicles through PEGylated lipid detergent-aided cell extrusion10.1039/D2NR04272J ↗
71. Requirement of zinc transporter ZIP10 for epidermal development: Implication of the ZIP10–p63 axis in epithelial homeostasis10.1073/pnas.1710726114 ↗
72. Nicotinamide mononucleotide reduces melanin production in aged melanocytes by inhibiting cAMP/Wnt signaling10.1016/j.jdermsci.2022.05.002 ↗
73. A systematic exploration reveals the potential of spermidine for hypopigmentation treatment through the stabilization of melanogenesis-associated proteins10.1038/s41598-022-18629-3 ↗
74. A systematic exploration of ginsenoside Rg5 reveals anti-inflammatory functions in airway mucosa cells10.1016/j.jgr.2022.06.001 ↗
75. Fluorescence-based assays for measuring doxorubicin in biological systems
76. The PRIDE database at 20 years: 2025 update10.1093/nar/gkae1011 ↗