Rapid tissue adhesive coat improves engraftment efficiency of stem cell therapy
Source abstract
Abstract
Stem cell therapy holds promise for treating various diseases, but low engraftment efficacy remains a significant hurdle. Most stem cells are unable to rapidly anchor in the pathological microenvironment, and these dissociated cells undergo anoikis. In this study, we engineer a tissue adhesive coat wearing adipose-derived stem cells (TAC-ADSCs) using a biotin-streptavidin-amplified o-nitrobenzene (NB). TAC-ADSCs can rapidly anchor at the target site. TAC-ADSCs exhibit prolonged retention time in the joint cavity, even on cartilage surfaces that are difficult for cells to adhere to, without affecting their viability and differentiation capacity. In a rabbit cartilage defect model and a mouse osteoarthritis (OA) model, we find that TAC-ADSCs exhibit better therapeutic effects than unmodified ADSCs, demonstrating the potential of this modification method for improving cell engraftment efficiency. This safe, facile, and efficient method for achieving rapid cell adhesion capability on tissue has important implications for enhancing the therapeutic effectiveness of stem cell therapy.
Source: openalex_inverted_index · Quality: baseline
Research context
OpenAlex topics: 3D Printing in Biomedical Research · Cellular Mechanics and Interactions · Electrospun Nanofibers in Biomedical Applications
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
References (38)
Show source-provided references
1. Stem Cell Therapies in Clinical Trials: Progress and Challenges10.1016/j.stem.2015.06.007 ↗
2. Tissue Cells Feel and Respond to the Stiffness of Their Substrate10.1126/science.1116995 ↗
3. Improving Cell Engraftment in Cardiac Stem Cell Therapy10.1155/2016/7168797 ↗
4. The use of type 1 collagen scaffold containing stromal cell-derived factor-1 to create a matrix environment conducive to partial-thickness cartilage defects repair10.1016/j.biomaterials.2012.10.027 ↗
5. Matrix Elasticity Directs Stem Cell Lineage Specification10.1016/j.cell.2006.06.044 ↗
6. Recent advance in surface modification for regulating cell adhesion and behaviors10.1515/ntrev-2020-0076 ↗
7. A Review of Cell Adhesion Studies for Biomedical and Biological Applications10.3390/ijms160818149 ↗
8. Revealing early steps of alpha2beta1 integrin-mediated adhesion to collagen type I by using single-cell force spectroscopy10.1091/mbc.e06-09-0777 ↗
9. Light-driven single-cell rotational adhesion frequency assay10.1186/s43593-022-00020-4 ↗
10. Investigation of Cell-Substrate Adhesion Properties of Living Chondrocyte by Measuring Adhesive Shear Force and Detachment Using AFM and Inverse FEA10.1038/srep38059 ↗
11. Engineering the Surface of Therapeutic “Living” Cells10.1021/acs.chemrev.7b00157 ↗
12. Chemically engineering cells for precision medicine10.1039/D2CS00142J ↗
13. Surface-Anchored Nanogel Coating Endows Stem Cells with Stress Resistance and Reparative Potency via Turning Down the Cytokine-Receptor Binding Pathways
14. Polymerization-Mediated Multifunctionalization of Living Cells for Enhanced Cell-Based Therapy10.1002/adma.202007379 ↗
15. Targeted Repair of Vascular Injury by Adipose-Derived Stem Cells Modified with P-Selectin Binding Peptide10.1002/advs.201903516 ↗
16. Programmed assembly of 3-dimensional microtissues with defined cellular connectivity10.1073/pnas.0900717106 ↗
17. Engineered osteoclasts as living treatment materials for heterotopic ossification therapy10.1038/s41467-021-26593-1 ↗
18. General and Facile Coating of Single Cells via Mild Reduction10.1021/jacs.7b08440 ↗
19. High-efficiency labeling of sialylated glycoproteins on living cells10.1038/nmeth.1305 ↗
20. Cell armor for protection against environmental stress: Advances, challenges and applications in micro- and nanoencapsulation of mammalian cells10.1016/j.actbio.2018.11.040 ↗
21. Enhancement of nucleus pulposus repair by glycoengineered adipose-derived mesenchymal cells10.1016/j.biomaterials.2022.121463 ↗
22. An artificial membrane binding protein-polymer surfactant nanocomplex facilitates stem cell adhesion to the cartilage extracellular matrix10.1016/j.biomaterials.2021.120996 ↗
23. Tropoelastin improves adhesion and migration of intra-articular injected infrapatellar fat pad MSCs and reduces osteoarthritis progression
24. Polyglutamic Acid-Based Elastic and Tough Adhesive Patch Promotes Tissue Regeneration through In Situ Macrophage Modulation10.1002/advs.202106115 ↗
25. A long-term retaining molecular coating for corneal regeneration
26. Tissue-Integratable and Biocompatible Photogelation by the Imine Crosslinking Reaction10.1002/adma.201505336 ↗
27. Probing the chemical structure of monolayer covalent-organic frameworks grown via Schiff-base condensation reactions10.1039/C6CC03895F ↗
28. Structural properties and surface morphology of laser-deposited amorphous carbon and carbon nitride films10.1016/S0257-8972(99)00591-5 ↗
29. Cell adhesion force microscopy10.1073/pnas.96.2.471 ↗
30. Morphology and mechanics of chondroid cells from human adipose-derived Stem cells detected by atomic force microscopy10.1007/s11010-012-1263-5 ↗
31. Adhesion strength and morphologies of rBMSCS during initial adhesion and spreading10.1016/S0894-9166(15)30045-8 ↗
32. Morphological control of cell growth and viability10.1002/bies.950191102 ↗
33. Characteristics of human adipose derived stem cells in scleroderma in comparison to sex and age matched normal controls: implications for regenerative medicine10.1186/s13287-016-0444-7 ↗
34. The meaning, the sense and the significance: translating the science of mesenchymal stem cells into medicine10.1038/nm.3028 ↗
35. Visualizing the Fate of Intra-Articular Injected Mesenchymal Stem Cells In Vivo in the Second Near-Infrared Window for the Effective Treatment of Supraspinatus Tendon Tears10.1002/advs.201901018 ↗
36. Surgical and tissue engineering strategies for articular cartilage and meniscus repair10.1038/s41584-019-0255-1 ↗
37. Adipose-derived mesenchymal stem cell therapy in the treatment of knee osteoarthritis: a randomized controlled trial10.2217/rme-2018-0161 ↗
38. The surgical destabilization of the medial meniscus (DMM) model of osteoarthritis in the 129/SvEv mouse10.1016/j.joca.2007.03.006 ↗