An engineered, non-diazotrophic cyanobacterium and its application in bioelectrochemical nitrogen fixation
Source abstract
Abstract
The reduction of chemically inert nitrogen to ammonia is a critical step in the global nitrogen cycle. Microbial nitrogen fixation is a promising way to realize nitrogen reduction and ammonia production at mild conditions. Here, we report an engineered, non-diazotrophic Synechococcus elongatus PCC 7942 strain with nitrogen fixation activity that is constructed by integrating a modified nitrogenase gene cluster into the genome. The engineered S. elongatus PCC 7942 strain is employed in a bioelectrochemical nitrogen-fixation (e-BNF) system for ammonia production. Because the e-BNF system supplies adequate external electrons for the turnover of nitrogenase, the nitrogen fixation activity of the engineered S. elongatus PCC 7942 strain is significantly improved. After 48 h of reaction, the e-BNF system accumulates 173 μM of NH3, which is 21 times higher than that generated from solely photosynthesis-driven nitrogen fixation, with faradaic efficiency of 6.85%. This work may provide new insight into biological nitrogen-fixation systems and ammonium production.
Source: openalex_inverted_index · Quality: baseline
Research context
OpenAlex topics: Ammonia Synthesis and Nitrogen Reduction · Microbial Fuel Cells and Bioremediation · Advanced Photocatalysis Techniques
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
References (67)
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