journal article·2023-07Open access

Field experimentation of bio-cementation using low-cost cementation media for preservation of slope surface

Sivakumar Gowthaman, Hiromu Koizumi, Kazunori Nakashima, Satoru Kawasaki

Abstract

Microbial induced carbonate precipitation (MICP) is a promising bio-cementation method that involves ureolytic bacteria to improve the geotechnical properties of soil. The laboratory-scale studies carried out in the recent past showed that the MICP can be a potential alternative for slope surface preservation. However, the use of analytical-grade chemicals makes this method too expensive, limiting the applicability of the method especially when implicated at field-scale. The purpose of this research work was to assess the effectiveness of using inexpensive low-grade chemicals for the in-situ stabilization of slope surface. Two test plots were established at the project site (Hokkaido expressway slope, Japan) and subjected to MICP treatment via surficial spraying. One was treated using the cementation media formulated by low-grade chemicals (inclusive of fertilizer urea, snow-melting agent and beer-yeast), while the typical analytical-grade media was used to treat the other plot. After 20 days of treatment, the treated slope surfaces were evaluated by surface strength, CaCO3 content, scanning electron microscopy, energy dispersive spectroscopy (EDS) and X-ray diffraction (XRD) analyses. The outcomes indicated that the surface was significantly improved by low-grade chemicals; a stiff surface layer was desirably formed to the depth of 5 – 10 cm with the surface strength and CaCO3 content in the ranges of 0.14 – 1.02 MPa and 0.56 – 3.7%, respectively. The results are compared and discussed, and the challenges in the use of low-grade chemicals are pointed out for the way forward. Cost analysis disclosed that the material cost of the cementation media was reduced by around thirty-seven-fold (by 97%) compared to the analytical-grade media. While demonstrating the potential use of low-grade chemicals, the field-scale experiment could contribute to narrow down the gap between the present-state and real-scale deployment of MICP technology.
Source: openalex_inverted_index · Quality: baseline

Research context

OpenAlex topics: Microbial Applications in Construction Materials · Grouting, Rheology, and Soil Mechanics · Marine Sponges and Natural Products

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

References (78)

Show source-provided references
1. Effect of simulated acid rain on the stability of calcium carbonate immobilized by microbial carbonate precipitation10.1016/j.jenvman.2020.110419 ↗
2. Study of the effect of temperature on microbially induced carbonate precipitation10.1007/s11440-018-0758-y ↗
3. Enhancement of MICP-Treated Sandy Soils against Environmental Deterioration10.1061/(ASCE)MT.1943-5533.0002959 ↗
4. Upscaling effects of soil improvement by microbially induced calcite precipitation by surface percolation10.1080/01490451.2013.836579 ↗
5. Influence of Confinement and Cementation Level on the Behavior of Microbial-Induced Calcite Precipitated Sands under Monotonic Drained Loading10.1061/(ASCE)GT.1943-5606.0001379 ↗
6. Mechanical behavior of sands treated by microbially induced carbonate precipitation10.1061/(ASCE)GT.1943-5606.0001383 ↗
7. Dynamic response of liquefiable sand improved by microbial-induced calcite precipitation10.1680/geot.SIP13.P.019 ↗
8. Freeze-thaw durability and shear responses of cemented slope soil treated by microbial induced carbonate precipitation10.1016/j.sandf.2020.05.012 ↗
9. Microbially induced cementation to control sand response to undrained shear10.1061/(ASCE)1090-0241(2006)132:11(1381) ↗
10. Influence of cementation level on the strength behaviour of bio-cemented sand10.1007/s11440-017-0574-9 ↗
11. Large-scale spatial characterization and liquefaction resistance of sand by hybrid bacteria induced biocementation10.1016/j.enggeo.2022.106635 ↗
12. Feasibility study of applying electrokinetic technology coupled with enzyme-induced carbonate precipitation treatment to Cu- and Pb-contaminated loess remediation10.1016/j.jclepro.2023.136734 ↗
13. Investigating immobilization efficiency of Pb in solution and loess soil using bio-inspired carbonate precipitation10.1016/j.envpol.2023.121218 ↗
14. Applicability of microbial calcification method for sandy-slope surface erosion control10.1061/(ASCE)MT.1943-5533.0002897 ↗
15. Effect of microbial-induced calcite precipitation on surface erosion and scour of granular soils proof of concept10.3141/2657-02 ↗
16. Application of microbially induced calcite precipitation in erosion mitigation and stabilisation of sandy soil foreshore slopes: a preliminary investigation10.1016/j.enggeo.2015.12.027 ↗
17. Application of microbially induced calcite precipitation to prevent soil loss by rainfall: effect of particle size and organic matter content
18. Stabilisation of crustal sand layer using biocementation technique for wind erosion control10.1016/j.aeolia.2019.06.001 ↗
19. Strength and durability of biocemented sands: Wetting-drying cycles, ageing effects, and liquefaction resistance10.1016/j.geoderma.2021.115359 ↗
20. Effect of wetting and drying cycles on the durability of bio-cemented soil of expressway slope10.1007/s13762-021-03306-1 ↗
21. Durability analysis of bio-cemented slope soil under the exposure of acid rain10.1007/s11368-021-02997-w ↗
22. Lactose mother liquor as an alternative nutrient source for microbial concrete production by Sporosarcina pasteurii10.1007/s10295-008-0514-7 ↗
23. Low-cost cultivation of Sporosarcina pasteurii strain in food-grade yeast extract medium for microbially induced carbonate precipitation (MICP) application10.1016/j.bcab.2018.11.030 ↗
24. Process economics
25. Whiffin · 2004
26. Corn steep liquor as a nutritional source for biocementation and its impact on concrete structural properties10.1007/s10295-018-2050-4 ↗
27. A cost effective cultivation medium for biocalcification of Bacillus pasteurii KCTC 3558 and its effect on cement cubes properties10.1016/j.micres.2016.03.010 ↗
28. Biogeotechnical approach for slope soil stabilization using locally isolated bacteria and inexpensive low-grade chemicals: a feasibility study on Hokkaido expressway soil, Japan10.1016/j.sandf.2018.12.010 ↗
29. Effect of bio-cementation on the strength behaviour of clay soils using egg shell as calcium source10.1007/s12665-022-10475-w ↗
30. Biocementation for sand using an eggshell as calcium source10.1061/(ASCE)GT.1943-5606.0001534 ↗
31. Biocementation of calcareous sand using soluble calcium derived from calcareous sand10.1007/s10064-017-1106-4 ↗
32. Microbial Induced Calcium Carbonate Precipitation (MICP) Using Pig Urine as an Alternative to Industrial Urea10.1007/s12649-018-0324-8 ↗
33. Standard Practice for Classification of Soils for Engineering Purposes (Unified Soil Classification System)
34. Comparing performances of MICP bacterial vegetative cell and microencapsulated bacterial spore methods on concrete crack healing10.1016/j.conbuildmat.2021.124227 ↗
35. Evaluation of microencapsulation techniques for micp bacterial spores applied in self-healing concrete10.1038/s41598-019-49002-6 ↗
36. Investigation of the crack healing performance in mortar using microbially induced calcium carbonate precipitation (MICP) method10.1016/j.conbuildmat.2019.04.035 ↗
37. Stimulation of native microorganisms for biocementation in samples recovered from field scale treatment depths10.1061/(ASCE)GT.1943-5606.0001804 ↗
38. Microbially induced sand cementation method using Pararhodobacter sp. strain SO1, inspired by beachrock formation mechanism10.2320/matertrans.M-M2015842 ↗
39. Spectrophotometric determination of ammonia as indophenol10.1021/ac60172a034 ↗
40. A feasible scale-up production of Sporosarcina pasteurii using custom-built stirred tank reactor for in-situ soil biocementation10.1016/j.bcab.2020.101544 ↗
41. Selective enrichment and production of highly urease active bacteria by non-sterile (open) chemostat culture10.1007/s10295-013-1310-6 ↗
42. Treatment of sand using microbial-induced carbonate precipitation (MICP) for wind erosion application
43. Microbial induced calcite precipitation of dune sand using a surface spray technique
44. Microscale visualization of microbial-induced calcium carbonate precipitation processes10.1061/(ASCE)GT.1943-5606.0002079 ↗
45. Experimental study on near-surface stabilization of slopes in cold region using bio-mediated geotechnical engineering, Doctoral dissertation, Hokkaido University
46. Needle penetration test: evaluation of its performance and possible uses in predicting strength of weak and soft rocks10.1016/j.enggeo.2012.08.007 ↗
47. Japanese Standards and Explanations of Geotechnical and Geoenvironmental Investigation Methods (3431-2012)
48. Cementation of soils due to calcium carbonate
49. Factors affecting the performance of microbial-induced carbonate precipitation (MICP) treated soil: a review10.1007/s12665-020-8840-9 ↗
50. Quantifying biomediated ground improvement by ureolysis: large-scale biogrout experiment10.1061/(ASCE)GT.1943-5606.0000382 ↗
51. Microbial induced carbonate precipitation using a native inland bacterium for beach sand stabilization in nearshore areas10.3390/app9153201 ↗
52. Experimental optimization of microbial-induced carbonate precipitation for soil improvement10.1061/(ASCE)GT.1943-5606.0000787 ↗
53. Significant indicators for biomineralisation in sand of varying grain sizes10.1016/j.conbuildmat.2015.12.023 ↗
54. Study of biocementation treatment to prevent erosion by concentrated water flow in a small-scale sand slope
55. Effect of freeze-thaw cycles on engineering properties of biocemented sand under different treatment conditions10.1016/j.enggeo.2021.106022 ↗
56. Application of waste eggshell as a source of calcium in bacterial bio-cementation to enhance the engineering characteristics of sand10.1007/s11356-022-20484-8 ↗
57. Biocementation of sand by Sporosarcina pasteurii strain and technical-grade cementation reagents through surface percolation treatment method10.1016/j.conbuildmat.2019.116828 ↗
58. Effect of magnesium as substitute material in enzyme-mediated calcite precipitation for soil-improvement technique10.3389/fbioe.2016.00037 ↗
59. Effects of iron species and inert minerals on coagulation and direct filtration for humic acid removal10.1016/j.desal.2008.03.015 ↗
60. Spoilage yeasts in beer and beer products
61. Carbonate precipitation through microbial activities in natural environment, and their potential in biotechnology: a review10.3389/fbioe.2016.00004 ↗
62. Characterization of crude bacterial urease for CaCO3 precipitation and cementation of silty sand10.1061/(ASCE)MT.1943-5533.0003100 ↗
63. Comparison of soil improvement methods using crude soybean enzyme, bacterial enzyme or bacteria induced carbonate precipitation
64. Swell-shrink behaviour of cement with fly ash-stabilised lakebed sediment10.1007/s10064-020-02069-2 ↗
65. Effect of organic matter content on enzymatic biocementation process applied to coarse-grained soils10.1061/(ASCE)MT.1943-5533.0002774 ↗
66. Influence of humic acid on microbial induced carbonate precipitation for organic soil improvement
67. Cement stabilization of indian peat: an experimental investigation10.1061/(ASCE)MT.1943-5533.0003363 ↗
68. Improving mechanical properties and shrinkage cracking characteristics of soft clay in deep soil mixing10.1016/j.conbuildmat.2021.125858 ↗
69. Repurposing of stabilised dredged lakebed sediment in road base construction10.1007/s11368-021-02974-3 ↗
70. Effect of chemical treatment used in MICP on engineering properties of cemented soils10.1680/geot.SIP13.P.022 ↗
71. Effect of bacterial calcium carbonate precipitation on compressibility and shear strength of organic soil10.1016/j.sandf.2015.09.020 ↗
72. Calcium phosphate biocement using bone meal and acid urease: an eco-friendly approach for soil improvement10.1016/j.jclepro.2021.128782 ↗
73. Improvement of the shearing behaviour of loess using recycled straw fiber reinforcement10.1007/s12205-021-2263-3 ↗
74. Bioprecipitation of calcium carbonate mediated by ureolysis: a review
75. Efficiency of microbially-induced calcite precipitation in natural clays for ground improvement10.1016/j.conbuildmat.2021.122722 ↗
76. Use of microbially induced calcite precipitation for soil improvement in compacted clays10.1007/s40891-021-00327-1 ↗
77. Microbial carbonate precipitation as a soil improvement technique10.1080/01490450701436505 ↗
78. Investigation of pore-scale CaCO3 distributions and their effects on stiffness and permeability of sands treated by microbially induced carbonate precipitation (MICP)10.1016/j.sandf.2020.07.003 ↗