Oral delivery of insulin via mesoporous carbon nanoparticles for colonic release allows glycemic control in diabetic rats
Source abstract
Abstract
In this article, a new type of mesoporous carbon nanoparticles (MCN) was fabricated as a potential oral delivery system of insulin to reduce the adverse reactions by hypodermic injection. The mesoporous carbon nanoparticles-carried insulin (MCNI) was studied using scanning electron microscopy (SEM), transmission electron microscopy (TEM), and Fourier transform infrared spectroscopy (FT-IR) compared with the blank MCNs. The Brunauer–Emmett–Teller (BET) method was utilized to calculate the specific surface area. The pore volume and pore size distribution (PSD) curves were calculated by Barrett–Joyner–Halenda (BJH) model. The entrapment efficiency (EE%) and loading content (LC%) of insulin onto the MCNs were determined by RP-HPLC. In vitro insulin release from MCNI was determined in simulated intestinal fluid. To evaluate the pharmacodynamics of MCNIs orally, the variation of glycemia of diabetic rats after oral administration of MCNIs was compared with the rats receiving hypodermic injection of insulin. Besides, the absorption of FITC-labeled MCNs in HCT-116 cells was tested. The results showed that there is significant difference between MCNs and MCNIs through SEM, TEM, and FT-IR. The entrapment efficiency, loading content and in vitro insulin release met the requirements of the pharmacodynamic study. The specific surface area, pore volume and pore size of MCNIs were significantly decreased compared to that of MCNs. The pharmacodynamics study showed that the blood sugar level was significantly decreased after the oral administration of MCNIs. The FITC-labeled MCNs showed significant absorption in HCT-116 cells. The MCNIs were successfully synthesized with commendable entrapment efficiency and loading content which preferably decreased the blood sugar in diabetes rats via oral administration.
Source: openaire · Quality: candidate unverified · Source record ↗
Research context
OpenAlex topics: Advanced Drug Delivery Systems · Diabetes Management and Research · Nanoparticle-Based Drug Delivery
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References (27)
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1. WHO. Global status report on noncommunicable diseases 2014. http://www.wsmi.org/who-global-status-report-on-noncommunicable-diseases-2014/2015 . Accessed 1 Feb 2016
2. Fonte P, Araújo F, Silva C et al (2015) Polymer-based nanoparticles for oral insulin delivery: revisited approaches. Biotechnol Adv 6:33
3. Alibolandi M, Alabdollah F, Sadeghi F et al (2016) Dextran-b-poly (lactide-coglycolide) polymersome for oral delivery of insulin: In vitro and in vivo evaluation. J Control Release 227:58–7010.1016/j.jconrel.2016.02.031 ↗
4. Skyler JS, Jovanovic L, Klioze S et al (2007) Two-year safety and efficacy of inhaled human insulin (Exubera) in adult patients with type 1 diabetes. Diabetes Care 3(30):579–58510.2337/dc06-1863 ↗
5. Ismail R, Csóka I (2017) Novel strategies in the oral delivery of antidiabetic peptide drugs—Insulin, GLP 1 and its analogs. Eur J Pharm Biopharm 115:257–26710.1016/j.ejpb.2017.03.015 ↗
6. Jain D, Panda AK, Majumdar DK (2005) Eudragit S100 entrapped insulin microspheres for oral delivery. AAPS Pharm Sci Tech 1:6
7. Youn YS, Chae SY, Lee S et al (2008) Improved peroral delivery of glucagon-like peptide-1 by site-specific biotin modification: design, preparation, and biological evaluation. Eur J Pharm Biopharm 3:68
8. Kinesh VP, Neelam DP, Punit BP et al (2010) Novel approaches for oral delivery of insulin and current status of oral insulin products. Int J Pharm Sci Nanotechnol 3:3
9. Neha P, Tanuj J (2015) A review on novel approaches for oral delivery of insulin. J Drug Del Ther 4:5
10. Cilek A, Celebi N, Tirnaksiz F et al (2005) A lecithin-based microemulsion of insulin with aprotinin for oral administration: investigation of hypoglycemic effects in non-diabetic and STZ-induced diabetic rats. Int J Pharm 1:298
11. Milletti F (2012) Cell-penetrating peptides: classes, origin, and current landscape [J]. Drug Discov 15–16:17
12. Zhang D, Wang J, Xu D (2016) Cell-penetrating peptides as noninvasive transmembrane vectors for the development of novel multifunctional drug delivery systems [J]. J Control Release 229:130–13910.1016/j.jconrel.2016.03.020 ↗
13. Ramsey JD, Flynn NH (2015) Cell-penetrating peptides transport therapeutics into cells. Pharmacol Ther [J] 154:78–8610.1016/j.pharmthera.2015.07.003 ↗
14. Varshney HM, Kumar R, Mohan S (2015) Novel approaches for insulin delivery: current status. Int J Ther Appl 7:25–31
15. Shinde PV (2013) Novel carrier systems for oral delivery of insulin. Asian J Pharm Tech Innov 2:1
16. Mrsny RJ, Daugherty A (2010) Proteins and Peptides: Pharmacokinetic, Pharmacodynamic, and Metabolic Outcomes. Taylor & Francis Group, Boca Raton
17. Zhang Y, Wei W, Lv P et al (2011) Preparation and evaluation of alginate-chitosan microspheres for oral delivery of insulin. Eur J Pharm Biopharm 1:77
18. D’Souza B, Bhowmik T, Uddin MN et al (2015) Development of b- cyclodextrinbased sustained release microparticles for oral insulin delivery. Drug Dev Ind Pharm 8:41
19. Hasan AS, Sapin A, Damge C et al (2015) Reduction of the in vivo burst release of insulin-loaded microparticles. J Drug Del Sci Technol 30:486–49310.1016/j.jddst.2015.06.020 ↗
20. Suri SS, Fenniri H, Singh B (2007) Nanotechnology-based drug delivery systems. J Occup Med Toxicol 2:1610.1186/1745-6673-2-16 ↗
21. Yue Q, Zhang Y, Wang C et al (2015) Magnetic yolk-shell mesoporous silica microspheres with supported Au nanoparticles as recyclable high-performance nanocatalysts. J Mater 3:4586–4594
22. Lu AH, Sun T, Li WC et al (2011) Synthesis of discrete and dispersible hollow carbon nanospheres with high uniformity by using confined nanospace pyrolysis. Angew Chem Int 50:11765–1176810.1002/anie.201105486 ↗
23. Chung TH, Wu SH, Yao M et al (2007) The effect of surface charge on the uptake and biological function of mesoporous silica nanoparticles 3T3-L1 cells and human mesenchymal stem cells. Biomaterials 19:28
24. Wang T, Zhao P, Zhao Q et al (2016) The mechanism for increasing the oral bioavailability of poorly water-soluble drugs using uniform mesoporous carbon spheres as a carrier. Drug Deliv 2:23
25. Zhang Y, Zhu W, Zhang H et al (2017) Carboxymethyl chitosan/phospholipid bilayer-capped mesoporous carbon nanoparticles with pH-responsive and prolonged release properties for oral delivery of the antitumor drug, Docetaxel. Int J Pharm 1:532
26. Eleftheriadis GK, Filippousi M, Tsachouridou V et al (2016) Evaluation of mesoporous carbon aerogels as carriers of the non-steroidal anti-inflammatory drug ibuprofen. Int J Pharm 1–2:515
27. Zhang Y, Zhao Q, Zhu W et al (2015) Synthesis and evaluation of mesoporous carbon/lipid bilayer nanocomposites for improved oral delivery of the poorly water-soluble drug, nimodipine. Pharm Res 7:32