Change in the UV-VIS Absorbance of Amino Acids as a Result of Femtosecond Laser Irradiation

Problems such as genetic damage should be considered in the medical application of lasers.Photochemical damage to living tissue is caused by high-photon energy light.Changes in the UV-VIS absorbance of amino acids by femtosecond laser irradiation are investigated in the present study.Ultraviolet absorption spectra of amino acids were measured following their irradiation by femtosecond laser.The results indicate that changes in ultraviolet absorption appeared at a peak intensity above 70 GW/cm 2 , suggesting that a change in the chemical structure was caused by multiphoton absorption during the femtosecond laser irradiation.