Influence of Micro-nano Structure of Haydroxyapatite Particles on Protein Adsorption

Hydroxyapatite (HAP) particles were hydrothermally synthesized with the surface morphologies adjusted by cyclohexane-1, 2, 3, 4, 5, 6-hexacarboxylic acid (H 6 E) as the template. HAP particles were characterized by XRD, BET, SEM and FTIR. The protein adsorption-desorption behaviors of positively charged lysozyme (LYS), fibrinogen (FN) and negatively charged bovine serum albumin (BSA) on these HAP particles were examined. The results indicate that using H 6 E as a template to fabricate micro-nano structures on HAP particles through hydrothermal reaction is simple and controllable. HAP particles with micro-nano structures show selective protein adsorption-desorption properties for different proteins. The protein-loaded shell-like HAP particle (HAP50-protein) shows an excellent protein release behavior in vitro.