Isolation, identification, and pathogenic effects of Trichoderma spp. from Auricularia auricula
Source abstract
Abstract
Abstract Auricularia auricula, one of the most important edible mushrooms, is affected heavily by Trichoderma. We collected the diseased samples from the main A.auricula cultivation regions to characterize the pathogen and study the effect of Trichoderma spp. on A.auricula species. We identified one Trichoderma species, T.pleuroticola, based on the internal transcribed spacer and morphology characteristics and two types of A.auricula strains, Heiwei 15 (HW 15) and Hei 29 (H 29) were tested in this work. The growth rate of T.pleuroticola was 3.26–3.52 times higher than that of A.auricula and advantageously competed for living space and nutrients. In confrontation culture, T.pleuroticola completely inhibited the mycelium growth of A.auricula and grew on it, resulting in the diverse impact on HW 15 and H 29. In addition, T.pleuroticola can produce metabolites with antibacterial activity. The inhibition rate of volatile metabolites to H-29 and HW 15 was 13.46% and 10.44%, and the inhibition rate of nonvolatile metabolites to H-29 and HW 15 was 36.04% and 31.49%, respectively. These antifungal activities of inhibiting the growth of A.auricula were abbtributed to the organic compounds from T.pleuroticola, nonanal, tyrosine, beta-sitosterol, and wortmannin, which were identified by gas chromatography-ion mobility spectroscopy (GC-IMS) and liquid chromatography-mass spectrometry (LC-MS/MS). In short, T. pleuroticola was a highly pathogenic fungi in the production of A.auricula.
Source: openaire · Quality: candidate unverified · Source record ↗
Research context
OpenAlex topics: Fungal Biology and Applications · Mycorrhizal Fungi and Plant Interactions · Plant-Microbe Interactions and Immunity
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
References (36)
Show source-provided references
1. Hoyos-Carvajal L, Orduz S, Bissett J (2009) Genetic and metabolic biodiversity of Trichoderma from Colombia and adjacent neotropic regions. Fungal Genet Biol 462(9):615–63110.1016/j.fgb.2009.04.006 ↗
2. Su DQ, Ding LJ, He S (2018) Marine-derived Trichoderma species as a promising source of bioactive secondary metabolites. Mini-Rev Med Chem 18(20):1702–171310.2174/1389557518666180727130826 ↗
3. Reino JL, Guerrero RF, Hernandez-Galan R et al (2008) Secondary metabolites from species of the biocontrol agent Trichoderma. Phytochem Rev 7(1):89–12310.1007/s11101-006-9032-2 ↗
4. Chen RG (2008) Isolation, identification and biocontrol mechanisms of antagonistic Trichoderma spp. Fu Jian Agricultural and Forestry University
5. Park MS, Bae KS, Yu SH (2006) The new species of Trichoderma associated with green mold of oyster mushroom cultivation in Korea. Mycobiology 34(3):111–11310.4489/MYCO.2006.34.3.111 ↗
6. Jayalal RGU, Adikaram NKB (2007) Influence of Trichoderma harzianum metabolites on the development of green mould disease in the oyster mushroom. Ceylon J Sci 36:53–60
7. Wang GZ, Cao XT, Ma XL et al (2016) Diversity and effect of Trichoderma spp. associated with green mold disease on Lentinula edodes in China. MicrobiologyOpen 5(4):709–71810.1002/mbo3.364 ↗
8. Hatvani L, Kredics L, Allaga H et al (2017) First report of Trichoderma aggressivum F. aggressivum green mold on Agaricus bisporus in Europe. Plant Dis 101(6):1052–105310.1094/PDIS-12-16-1783-PDN ↗
9. Largeteau ML, Savoie JM (2010) Microbially induced diseases of Agaricus bisporus: biochemical mechanisms and impact on commercial mushroom production. Appl Microbiol Biotechnol 86(1):63–7310.1007/s00253-010-2445-2 ↗
10. Obrien M, Kavanagh K, Grogan H (2017) Detection of Trichoderma aggressivum in bulk phase III substrate and the effect of T. aggressivum inoculum, supplementation and substrate-mixing on Agaricus bisporus yields. Eur J Plant Pathol 147(1):199–20910.1007/s10658-016-0992-9 ↗
11. Kohl J, Kolnaar R, Ravensberg WJ (2019) Mode of action of microbial biological control agents against plant diseases: relevance beyond efficacy. Front Plant Science 1010.3389/fpls.2019.00845 ↗
12. Yan YH (2011) Rearch on Identification of Trichoderma of mushrooms and control of Trichoderma、Mycogone cercina. Fu Jian Agricultural and Forestry University
13. Kim CS, Park MS, Kim SC et al (2012) Identification of Trichoderma, a competitor of Shiitake mushroom (Lentinula edodes), and competition between Lentinula edodes and Trichoderma species in Korea. Plant Pathol J 28(2):137–14810.5423/PPJ.2012.28.2.137 ↗
14. Cao XT (2015) Preliminaly Study on Trichoderma Spp. Identification and incidence regularity associated with Lentinula edodes cultivation bag rot disease. Wuhan: Huazhong Agricultural University
15. Wu XP (2008) Identification pathogenic mechanism and control of Trichoderma spp. isolated from edible fungi. Fujian: Fujian Agricultural Forestry University
16. Hatvani L, Sabolic P, Kocsube S et al (2012) The first report on mushroom green mould disease in Croatia. Arh Hig Rada Toksikol 63(4):481–48710.2478/10004-1254-63-2012-2220 ↗
17. Muskhazli M, Faridah QZ, Salfarina R et al (2006) The evidence of N-glycan linked mannose in exochitinase 42kda, from Trichoderma harzianum BIO10671 glycosylation. Malays J Microbiol 2(2):37–41
18. Yang HT (2009) Classification and identification of Trichoderma. China Dadi Press, Beijing, p 389
19. Kumar S, Stecher G, Tamura K (2016) MEGA 7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 33(7):1870–187410.1093/molbev/msw054 ↗
20. Owaid MN, Alsaeedi SSS, Abed IA et al (2017) Antifungal activities of some Pleurotus species (higher Basidiomycetes). Walailak J Sci Tech 14(3):215–224
21. Ebadzadsahrai G, Keppler EAH, Soby SD et al (2020) Inhibition of fungal growth and induction of a novel volatilome in response to Chromobacterium vaccinii volatile organic compounds. Front Microbiol 11:1035–103510.3389/fmicb.2020.01035 ↗
22. Stracquadanio C, Quiles JM, Meca G et al (2020) Antifungal activity of bioactive metabolites produced by Trichoderma asperellum and Trichoderma atroviride in liquid medium. J Fungi 6(4):26310.3390/jof6040263 ↗
23. Li GL, Xu Y, Xing PJ et al (2019) Pathogenicity of Trichoderma spp. to Lentinula edodes and the inhibitory effects of biocontrol agents. Mol Plant Breed 17(19):6530–6534
24. Wang Y, Liu ZY, Feng Y et al (2021) Study on Trichoderma infection process in Lentinus edodes strain production. Seed 40(6):131–141
25. Marik T, Urban P, Tyagi C et al (2017) Diversity profile and dynamics of peptaibols produced by green mould Trichoderma species in interactions with their hosts Agaricus bisporus and Pleurotus ostreatus. Chem Biodivers 14(6):1–2110.1002/cbdv.201700033 ↗
26. Innocenti G, Montanari M, Righini H et al (2019) Trichoderma species associated with green mould disease of Pleurotus ostreatus and their sensitivity to prochloraz. Plant Pathol 689(2):392–39810.1111/ppa.12953 ↗
27. Bengtsson G, Hedlund K, Rundgren S (1991) Selective odor perception in the soil Collembolaonychiurus armatus. J Chem Ecol 17(11):2113–212510.1007/BF00987995 ↗
28. Niu QH, Huang XW, Zhang L et al (2010) A Trojan horse mechanism of bacterial pathogenesis against nematodes. Proc Natl Acad Sci 107(38):16631–1663610.1073/pnas.1007276107 ↗
29. Fernando WGD, Ramarathnam R, Krishnamoorthy AS et al (2005) Identification and use of potential bacterial organic antifungal volatiles in biocontrol. Soil Biol Biochem 37(5):955–96410.1016/j.soilbio.2004.10.021 ↗
30. Ando H, Hatanaka K, Ohata I et al (2012) Antifungal activities of volatile substances generated by yeast isolated from Iranian commercial cheese. Food Control 26(2):472–47810.1016/j.foodcont.2012.02.017 ↗
31. Ando H, Kurata A, Kishimoto N (2015) Antimicrobial properties and mechanism of volatile isoamyl acetate, a main flavour component of Japanese sake (Ginjo-Shu). J Appl Microbiol 118(4):873–88010.1111/jam.12764 ↗
32. Lee S, Yap M, Behringer G et al (2016) Volatile organic compounds emitted by Trichoderma species mediate plant growth. Fungal Biol Biotechnol 3:710.1186/s40694-016-0025-7 ↗
33. Zhao P (1997) Effects of amino acids on the growth and development of black fungus Mycelium. J Gansu Agric Univ 2:61–64
34. Brian PW, Curtis PJ, Hemming HG et al (1957) Wortmannin, an antibiotic produced by Penicillium wortmanni. Trans Br Mycol Soc 40(3):365–36810.1016/S0007-1536(57)80033-3 ↗
35. Ahluwalia V, Kumar J, Rana VS et al (2015) Comparative evaluation of two Trichoderma harzianum strains for major secondary metabolite production and antifungal activity natural product research. Nat Prod Res 29(10):914–92010.1080/14786419.2014.958739 ↗
36. Xuan QC, Huang R, Miao CP et al (2014) Secondary metabolites of endophytic fungus Trichoderma sp. YM 311505 of Azadirachta indica. Chem Nat Compd 50(1):139–14110.1007/s10600-014-0891-2 ↗