An in vitro comparative study of layered-double hydroxide nanoconjugate in the delivery of small interference and short-hairpin ribonucleic acid

Alzheimer’s disease is a disease which cannot be cured completely. In this aspect ribonucleic acid interference (RNAi) therapy is a prospective therapeutic mechanism which can be used for identifying a future curative procedure. RNAi therapy comprises small interfering RNA (siRNA), short hairpin (shRNA) and micro-RNA therapeutics. Within these three mechanisms we have identified two of them as an effective method of combating this genetic incurable disease. siRNAs and shRNAs are very much effective in vitro that is already proved in many research work. In our study we have used a very potent, biocompatible nanoparticle-layered double hydroxide for delivering these macromolecules. However, the intercalation and cellular internalization of these macromolecules demonstrated significant differences. As siRNAs have low-molecular weight than shRNAs they demonstrated different characteristics in the case of internalization within layered-double hydroxide and while cellular internalization. At the end of this study it has been found that both of these macromolecules may be used as a therapeutic approach of Alzheimer’s disease after studying it in future in animal and human subjects.