Study of physicochemical properties of nanohydroxyapatite–chitosan composites

Hydroxyapatite nanoparticles were synthesized using modified sol–gel approach. Its biocompatibility was proved by in vitro experiments with diploid fibroblasts. The experiment showed that the presence of hydroxyapatite in the environment of cells does not reduce their viability. The observed phase transition of hydroxyapatite was explained as order/disorder transition, saving the crystal structure symmetry. Organo–inorganic composites of chitosan and hydroxyapatite were obtained by preparing a joint solution and pouring onto a glass substrate. According to XRD and DTA data, addition of hydroxyapatite reduces the softening and decomposition temperature of chitosan. This information is necessary to optimize the manufacturing processes of organo–inorganic scaffolds.