Visualizing Molecular Chaperone Controlled Resilient Cell Traction Force by Micropost Arrays Fabricated by Two-Photon Initiated Polymerization
Source abstract
Abstract
Freestanding compliant micropost arrays were fabricated on a glass surface by two-photon-initiated polymerization of polyacrylamide gels. Subcellular force exerted on each post was evaluated from the independent displacement of the post. The elasticity of the gel (57 kPa) was designed to be as compliant as biological tissues. We tried this array to reproduce the in situ behavior of L6 rat myoblasts. Cells stayed still extending pseudopodia exerting traction force. When the expression of one of the molecular chaperones, αB-crystallin was knocked down, the cells kept wandering showing round-shaped contours confirming the significance of αB-crystallin in maintaining subcellular attachment to extracellular matrix. We consider the micropost arrays suitable for subcellular analyses of mechanical behavior at in situ-relevant conditions.
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Research context
OpenAlex topics: Cellular Mechanics and Interactions · Tendon Structure and Treatment · 3D Printing in Biomedical Research
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Material Health Science Laboratory, Graduate School of Engineering, Tokyo University of Agriculture and Technology
Division of Applied Chemistry, Graduate School of Engineering, Tokyo University of Agriculture and Technology
Department of Applied Physics, Faculty of Engineering, Tokyo University of Agriculture and Technology
References (25)
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