Preparation and Characterisation of Cyclodextrin Glucanotransferase Enzyme Immobilised in Electrospun Nanofibrous Membrane
Source abstract
Abstract
An industrial enzyme, Cyclodextrin glucanotransferase (CGTase), was immobilised in polyvinyl alcohol (PVA) nanofiber (average diameter around 200 nm) membrane via co-electrospinning of the CGTase/PVA mixture followed with glutaraldehyde vapour phase cross-linking. Addition of enzyme with concentration ranging from 1.5 to 7.5 % to the PVA solution (8 wt%) caused significant changes to the liquid jet behaviours which consequently affected the nanofiber structures and sizes. Incorporation of CGTase in the PVA membrane was confirmed by Raman spectroscopic analysis. The Raman spectra also showed no structural changes occured to the enzyme after subjected to the electrostatic spinning and cross-linking reaction. The immobilised enzyme showed excellent catalytic efficiency with up to 3.6 times higher enzyme loading, 25 % higher activity and good reusability in comparison with CGTase/PVA film made up from the same starting solution (control).
Source: openalex_inverted_index · Quality: baseline
Research context
OpenAlex topics: Electrospun Nanofibers in Biomedical Applications · Graphene and Nanomaterials Applications · Enzyme Catalysis and Immobilization
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Graduate School of Bio-Applications and Systems Engineering, Tokyo University of Agriculture and Technology
Biotechnology Research Institute, Universiti Malaysia Sabah
Department of Process and Food Engineering, Faculty of Engineering, Universiti Putra Malaysia
Faculty of Chemical Engineering, Universiti Teknologi MARA
School of Energy and Environment, City University of Hong Kong
Department of Chemical Engineering, Tokyo University of Agriculture and Technology
References (36)
Show source-provided references
1. [1] Agarwal, S., Wendorff, J. H., & Greiner, A. Use of electrospinning technique for biomedical applications. Polymer, 49(26), 5603–5621 (2008).10.1016/j.polymer.2008.09.014 ↗
2. [2] Angammana, C. A Study of the Effects of Solution and Process Parameters on the Electrospinning Process and Nanofiber Morphology. IEEE Transactions on Industry Applications, 47(3), 1109–1117 (2011).10.1109/TIA.2011.2127431 ↗
3. [3] Dai, Y., Niu, J., Yin, L., Xu, J., & Xu, J. Laccasecarrying electrospun fibrous membrane for the removal of polycyclic aromatic hydrocarbons from contaminated water. Separation and Purification Technology, 104, 1–8 (2013).10.1016/j.seppur.2012.11.013 ↗
4. [4] Del Valle, E. M. M. Cyclodextrins and their uses: a review. Process Biochemistry, 39(9), 1033–1046 (2004).
5. [5] Fang, Y., Huang, X.-J., Chen, P.-C., & Xu, Z.-K. Polymer materials for enzyme immobilization and their application in bioreactors. BMB Reports, 44(2), 87–95 (2011).10.5483/BMBRep.2011.44.2.87 ↗
6. [6] Fazel, R., Torabi, S. F., Naseri-Nosar, P., Ghasempur, S., Ranaei-Siadat, S. O., & Khajeh, K. Electrospun polyvinyl alcohol/bovine serum albumin biocomposite membranes for horseradish peroxidase immobilization. Enzyme and Microbial Technology, 93–94, 1–10 (2016).10.1016/j.enzmictec.2016.07.002 ↗
7. [7] Herricks, T. E., Kim, S.-H., Kim, J., Li, D., Kwak, J. H., Grate, J. W., …Xia, Y. Direct fabrication of enzyme-carrying polymer nanofibers by electrospinning. Journal of Materials Chemistry, 15 (31), 3241–3245 (2005).10.1039/b503660g ↗
8. [8] Higuti, I. H., Anunciação, P., Papp, J., & De, V. M. Colorimetric Determination of α and β cyclodextrins and Studies on Optimization of CGTase Production from B . firmus Using Factorial Designs. Brazilian Archives of Biology and Technology, 47(6), 837–841 (2004).10.1590/S1516-89132004000600001 ↗
9. [9] Homaei, A. A., Sariri, R., Vianello, F., & Stevanato, R.Enzyme immobilization: An update. Journal of Chemical Biology, 6(4), 185–205 (2013).
10. [10] Huang, Z.-M., Zhang, Y.-Z., Kotaki, M., & Ramakrishna, S. A review on polymer nanofibers by electrospinning and their applications in nanocomposites. Composites Science and Technology,63(15), 2223–2253 (2003).10.1016/S0266-3538(03)00178-7 ↗
11. [11] Hwang, E. T., & Gu, M. B. Enzyme stabilization by nano/microsized hybrid materials. Engineering in Life Sciences, 13(1), 49–61 (2013).10.1002/elsc.201100225 ↗
12. [12] Ivanova, V. Immobilization of cyclodextrin glucanotransferase from paenibacillus macerans ATCC 8244 on magnetic carriers and production of cyclodextrins. Biotechnology and Biotechnology Equipment, 24(SE), 516–528 (2010).10.1080/13102818.2010.10817893 ↗
13. [13] Jochems, P., Satyawali, Y., Diels, L., & Dejonghe, W.Enzyme immobilization on/in polymeric membranes: status, challenges and perspectives in biocatalytic membrane reactors (BMRs). Green Chemistry, 13(7), 1609–1623 (2011).
14. [14] Kim, K.-O., & Kim, B.-S. Immobilization of Glucose Oxidase on a PVA/PAA nanofiber matrix reduces the effect of the hematocrit levels on a glucose biosensor. Journal of Fiber Science and Technology, 73(1), 27–33 (2017).10.2115/fiberst.fiberst.2017-0004 ↗
15. [15] Li, D., & Xia, Y. Electrospinning of Nanofibers:Reinventing the Wheel? Advanced Materials, 16 (14), 1151–1170 (2004).
16. [16] Li, Z., Wang, M., Wang, F., Gu, Z., Du, G., Wu, J., & Chen, J. γ-Cyclodextrin: A review on enzymatic production and applications. Applied Microbiology and Biotechnology, 77(2), 245–255 (2007).
17. [17] Martin, M. T., Alcalde, M., Plou, F. J., & Ballesteros, A. Covalent immobilization of cyclodextrin glycosyltransferase (CGTase) in activated silica and Sepharose. Indian Journal of Biochemistry & Biophysics, 39, 229–234 (2002).
18. [18] Ming, M., Kuroiwa, T., Ichikawa, S., Sato, S., & Mukataka, S. Production of Chitosan Oligosaccharides at High Concentration by Immobilized Chitosanase. Food Sci. Technol. Res.,12(2), 85–90 (2006).10.3136/fstr.12.85 ↗
19. [19] Moradzadegan, A., Ranaei-Siadat, S.-O., EbrahimHabibi, A., Barshan-Tashnizi, M., Jalili, R., Torabi, S. -F. , & Khajeh, K. Immobilization of acetylcholinesterase in nanofibrous PVA/BSA membranes by electrospinning. Engineering in Life Sciences, 10(1), 57–64 (2010).10.1002/elsc.200900001 ↗
20. [20] Moreno-Cortez, I. E., Romero-Garcia, J., GonzalezGonzalez, V., Garcia-Gutierrez, D. I., Garza Navarro, M. A., & Cruz-Silva, R. Encapsulation and immobilization of papain in electrospun nanofibrous membranes of PVA cross-linked with glutaraldehyde vapor. Materials Science & Engineering. C, Materials for Biological Applications, 52, 306–314 (2015).10.1016/j.msec.2015.03.049 ↗
21. [21] Morozov, V. N. Electrospray Deposition of Biomolecules. In Advanced Biochemical Engineering /Biotechnology (pp. 115–162). Berlin, Heidelberg:Springer Berlin Heidelberg (2009).10.1007/10_2008_44 ↗
22. [22] Oktay, B., Demir, S., & Kayaman-Apohan, N. Immobilization of α-amylase onto poly(glycidyl methacrylate) grafted electrospun fibers by ATRP. Materials Science and Engineering C, 50,386–393 (2015).10.1016/j.msec.2015.02.033 ↗
23. [23] Prosanov, I. Y., & Matvienko, A. A. Study of PVA thermal destruction by means of IR and Raman spectroscopy. Physics of the Solid State, 52(10),2203–2206 (2010).10.1134/S1063783410100318 ↗
24. [24] Reneker, D. H., & Yarin, A. L. Electrospinning jets and polymer nanofibers. Polymer, 49(10), 2387– 2425 (2008).10.1016/j.polymer.2008.02.002 ↗
25. [25] Saallah, S., Naim, M. N., Lenggoro, I. W., Mokhtar, M.N., Abu Bakar, N. F., & Gen, M. Immobilisation of cyclodextrin glucanotransferase into polyvinyl alcohol (PVA) nanofibers via electrospinning. Biotechnology Reports, 10, 44–48 (2016).10.1016/j.btre.2016.03.003 ↗
26. [26] Sakai, S., Antoku, K., Yamaguchi, T., & Kawakami, K.Transesterification by lipase entrapped in electrospun poly(vinyl alcohol) fibers and its application to a flow-through reactor. Journal of Bioscience and Bioengineering, 105(6), 687–689 (2008).10.1263/jbb.105.687 ↗
27. [27] Sardar, M., & Ahmad, R. Enzyme Immobilization:An Overview on Nanoparticles as Immobilization Matrix. Biochemistry & Analytical Biochemistry, 4,178 (2015).
28. [28] Sawada, K., Sakai, S., & Taya, M. Enhanced productivity of electrospun polyvinyl alcohol nanofibrous mats using aqueous N, Ndimethylformamide solution and their application to lipase-immobilizing membrane-shaped catalysts. Journal of Bioscience and Bioengineering,114(2), 204–208 (2012).10.1016/j.jbiosc.2012.03.017 ↗
29. [29] Sill, T. J., & von Recum, H. A. Electrospinning:Applications in drug delivery and tissue engineering. Biomaterials, 29(13), 1989–2006 (2008).
30. [30] Supaphol, P., Suwantong, O., & Sangsanoh, P. Electrospinning of Biocompatible Polymers and Their Potentials in Biomedical Applications. Advanced Polymer Science, 246(September 2011),213–239 (2012).10.1007/12_2011_143 ↗
31. [31] Tran, D. N., & Balkus, K. J. Enzyme Immobilization via Electrospinning. Topics in Catalysis, 55(16–18), 1057–1069 (2012).10.1007/s11244-012-9901-4 ↗
32. [32] Wang, Y., & Hsieh, Y.-L. Immobilization of lipase enzyme in polyvinyl alcohol (PVA) nanofibrous membranes. Journal of Membrane Science, 309(1–2),73–81 (2008).10.1016/j.memsci.2007.10.008 ↗
33. [33] Wen, Z. Raman Spectroscopy of Protein Pharmaceuticals. Journal of Pharmaceutical Sciences, 96(11), 2861–2878 (2007).10.1002/jps.20895 ↗
34. [34] Sciences96Wu, L., Yuan, X., & Sheng, J. Immobilization of cellulase in nanofibrous PVA membranes by electrospinning. Journal of Membrane Science, 250 (1–2), 167–173 (2005).10.1016/j.memsci.2004.10.024 ↗
35. [35] Zander, N. Hierarchically Structured Electrospun Fibers. Polymers, 5(1), 19–44 (2013).10.3390/polym5010019 ↗
36. [36] Zong, X., Kim, K., Fang, D., Ran, S., Hsiao, B. S., & Chu, B. Structure and process relationship of electrospun bioabsorbable nanofiber membranes. Polymer, 43, 4403–4412 (2002).10.1016/S0032-3861(02)00275-6 ↗